Structural characterization and immunostimulating activity of a levan-type fructan from Curcuma kwangsiensis

Dong, Caixia; Zhang, Lijiao; Xu, Rong; Zhang, Gong; Zhou, Yingbiao; Han, Xiaoqiang; Zhang, Ying; Sun, Yaqin

doi:10.1016/j.ijbiomac.2015.03.009

Cited by 56 publications

(26 citation statements)

References 12 publications

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“…The phagocytic activity of fresh garlic might be associated with fructan, and it is indicated that during the fermentation treatment, fructan constituents of garlic has degraded and basically no phagocytic effect can be found in black garlic. Moreover, it is revealed that fructan from Curcuma kwangsiensis shows significant immunostimulatory activity based on its ability to stimulate macrophage proliferation and enhance phagocytosis (Dong and others ). Furthermore, it is reported in previous study that fructan displays immunomodulatory activity on macrophage via toll‐like receptor 4 signaling (Xu and others ).…”

Section: Resultsmentioning

confidence: 99%

Comparison of Immunomodulatory Effects of Fresh Garlic and Black Garlic Polysaccharides on RAW 264.7 Macrophages

Yan

et al. 2017

Journal of Food Science

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Garlic has a long history to be used for medicine and food purposes. Black garlic, the fermented product of fresh garlic, is considered with better biological activities, such as antioxidant activity, and is developed as an increasingly popular functional food. Polysaccharides are the major components of fresh and black garlic, and immunomodulatory activity is one major pharmacological effect of polysaccharides. Therefore, chemical characteristics and immunomodulatory effects of polysaccharides from fresh and black garlic are investigated and compared in vitro for the 1st time, in order to reveal their molecular and pharmacological differences. It is demonstrated that the molecular weights of polysaccharides from the 2 sources and molar ratios of monosaccharides after acid hydrolysis are greatly variant. The effects of polysaccharides from 2 sources on RAW 264.7 macrophages functions, including promotion of phagocytosis, release of NO, and expressions of several immune-related cytokines (including interleukin [IL]-6, IL-10, tumor necrosis factor alpha, and interferon gamma), were different from each other. The results indicated that fresh garlic polysaccharide exhibited stronger immunomodulatory activities than that of black garlic. Moreover, it is revealed that fructan might be the bioactive component in garlic and it is indicated that during the fermentation treatment, fructan constituents of garlic has degraded, and basically no immunomodulatory effect can be found in black garlic polysaccharides.

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Section: Resultsmentioning

confidence: 99%

Comparison of Immunomodulatory Effects of Fresh Garlic and Black Garlic Polysaccharides on RAW 264.7 Macrophages

Yan

et al. 2017

Journal of Food Science

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“…Fructans are often found in large concentrations in underground storage organs of geophytes such as Galanthus nivalis (Amaryllidaceae) and Allium species (Alliaceae), where they can constitute more than 40% of the dry mass (Orthen & Wehrmeyer, ; Ranwala & Miller, ). In Curcuma kwangsiensis (Zingiberaceae), Dong et al () identified levan‐type fructans (average DP 30–31) with single fructose side chains (β‐2,1 linkage) and significant immunostimulating activity based on phagocytosis and macrophage proliferation assays. Moreover, inulin‐type fructans from Pfaffia glomerata (Amaranthaceae) roots were shown to have prebiotic effects (Caleffi et al, ).…”

Section: Fructans In Plantsmentioning

confidence: 99%

The fructan syndrome: Evolutionary aspects and common themes among plants and microbes

Versluys

Kırtel

Öner

et al. 2017

Plant Cell & Environment

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Fructans are multifunctional fructose-based water soluble carbohydrates found in all biological kingdoms but not in animals. Most research has focused on plant and microbial fructans and has received a growing interest because of their practical applications. Nevertheless, the origin of fructan production, the so-called "fructan syndrome," is still unknown. Why fructans only occur in a limited number of plant and microbial species remains unclear. In this review, we provide an overview of plant and microbial fructan research with a focus on fructans as an adaptation to the environment and their role in (a)biotic stress tolerance. The taxonomical and biogeographical distribution of fructans in both kingdoms is discussed and linked (where possible) to environmental factors. Overall, the fructan syndrome may be related to water scarcity and differences in physicochemical properties, for instance, water retaining characteristics, at least partially explain why different fructan types with different branching levels are found in different species.Although a close correlation between environmental stresses and fructan production is quite clear in plants, this link seems to be missing in microbes. We hypothesize that this can be at least partially explained by differential evolutionary timeframes for plants and microbes, combined with potential redundancy effects.

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“…However, a few studies about the medicinal plants or ingredients against grouper iridoviral infection are carried out. As a traditional Chinese medicine, C. kwangsiensis has excellent biological activities, including immunological activities (Dong et al, ), anticancer (Chen et al, ; Zeng et al, ), antioxidant and anti‐inflammatory activities (Tohda et al, ). Based on the valuable properties and abundance of C. kwangsiensis , the present study focuses on the antiviral effects of C. kwangsiensis against grouper Iridovirus (SGIV‐Gx) in vitro and in vivo, for the possible application of C. kwangsiensis ingredients as antiviral agents against grouper Iridovirus in aquaculture in the future.…”

Section: Discussionmentioning

confidence: 99%

“…The dried rhizome of C. kwangsiensis is an important herbal drug in traditional Chinese medicine and used to treat obstruction of blood circulation (Chen et al, ; Sasaki et al, ). Up to now, the variety of biological activities of C. kwangsiensis ingredients has been found, such as antitumor (Zeng et al, ), anti‐inflammatory (Tohda, Nakayama, Hatanaka, & Komatsu, ) and immunological effects (Dong et al, ), which make C. kwangsiensis possess promising therapeutic perspectives.…”

Section: Introductionmentioning

confidence: 99%

Antiviral abilities of Curcuma kwangsiensis ingredients against grouper iridoviral infection in vitro and in vivo

et al. 2019

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As one of the most serious pathogens in mariculture, the outbreaks of grouper Iridovirus (SGIV-Gx) caused high mortality rates in cultured groupers in Guangxi, China. Hence, effective medicines for fighting against grouper Iridovirus are urgently needed. The possible application of Curcuma kwangsiensis ingredients against SGIV-Gx infection was evaluated in vitro and in vivo in this study. The safe working concentration of each C. kwangsiensis ingredient was identified (C. kwangsiensis ethanol ingredient, CKEE ≤5 mg/ml; curcumin ≤20 μg/ml; curdione ≤500 μg/ml; curcumenol ≤500 μg/ml; curcumol displayed no cytotoxic effects even at 2 mg/ml) in vitro and in vivo. The inhibitory activities of each C. kwangsiensis ingredient against SGIV-Gx infection were analysed using aptamer (Q2)-based fluorescent molecular probe (Q2-AFMP) and RT-qPCR. The results showed that C. kwangsiensis ingredient (CKEE, curcumin, curcumol, curdione and curcumenol) displayed antiviral activities against SGIV-Gx infection in a dose-dependent manner. Furthermore, according to the inhibitory percentage analysed using RT-qPCR results, CKEE and curdione had the best antiviral activity against SGIV-Gx above 93%. Overall, the results suggest that some C. kwangsiensis ingredients have excellent antiviral effects, making it an interesting candidate for developing effective medicines for preventing and controlling SGIV-Gx outbreaks in mariculture.

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Structural characterization and immunostimulating activity of a levan-type fructan from Curcuma kwangsiensis

Cited by 56 publications

References 12 publications

Comparison of Immunomodulatory Effects of Fresh Garlic and Black Garlic Polysaccharides on RAW 264.7 Macrophages

Comparison of Immunomodulatory Effects of Fresh Garlic and Black Garlic Polysaccharides on RAW 264.7 Macrophages

The fructan syndrome: Evolutionary aspects and common themes among plants and microbes

Antiviral abilities of Curcuma kwangsiensis ingredients against grouper iridoviral infection in vitro and in vivo

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