Structural characterization and discrimination of Ophiopogon japonicas (Liliaceae) from different geographical origins based on metabolite profiling analysis

Lyu, Chao-Geng; Kang, Chuan-Zhi; Kang, Liping; Yang, Jian; Wang, Sheng; He, Yan; Deng, Aiping; Wang, Hongyang; Huang, Luqi; Guo, Lan-Ping

doi:10.1016/j.jpba.2020.113212

Cited by 20 publications

(14 citation statements)

References 22 publications

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“…The present study focused on wheat lipids instead of other higher-concentrated components such as wheat proteins or starches because this minor fraction has been less investigated to date than the major ones [14,15]. Moreover, from the perspective of omics, some most relevant differences in the upper reaches of omics towards exogenous factors (like growing location, weather and soil composition) might be detected and amplified investigating differences at small-molecule metabolome level [16,17], at which most studies focused on geographical discrimination of crops were investigated [18][19][20]. Previous researches have revealed that wheat lipids could be influenced by geological factors, thus wheat samples harvested from different origins might have specific lipid constitutes, which may affect the processing characteristics [21,22].…”

Section: Introductionmentioning

confidence: 99%

Lipid Compositions and Geographical Discrimination of 94 Geographically Authentic Wheat Samples Based on UPLC-MS with Non-Targeted Lipidomic Approach

Jin

Zheng

Zhang

et al. 2020

Foods

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Wheat is the staple food for the world’s major populations. However, chemical characters of geographically authentic wheat samples, especially for the lipids, have not been deeply studied. The present research aimed to investigate lipid compositions of Chinese wheat samples and clarify the major markers that contribute to the geographical differences. A total of 94 wheat samples from eight main wheat-producing provinces in China were evaluated to differentiate their lipid compositions. Based on the data collected from ultra-high-performance-liquid-chromatography tandem time-of-flight mass spectrometry (UPLC-Q/TOF MS), an optimized non-targeted lipidomic method was utilized for analyses. As the results, 62 lipid compounds, including fatty acids, phospholipids, galactolipids, triglycerides, diglycerides, alkylresorcinol, and ceramide were tentatively identified. Partial least squares discriminant analysis (PLS-DA) demonstrated a more satisfying performance in distinguishing wheat samples from different origins compared with principal component analysis (PCA). Further, the abundances of triglycerides and glycerophospholipids with more unsaturated fatty acids were found greater in wheat samples from northern origins of China, while more glycolipids and unsaturated fatty acids arose in southern original wheat samples. These findings describe the lipid profiles of wheat samples in China and could contribute to the quality and safety control for the wheat flour products.

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Section: Introductionmentioning

confidence: 99%

Lipid Compositions and Geographical Discrimination of 94 Geographically Authentic Wheat Samples Based on UPLC-MS with Non-Targeted Lipidomic Approach

Jin

Zheng

Zhang

et al. 2020

Foods

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“…The band of Rf 0.46 was found to be the characteristic component in younger samples while the bands of Rf 0.13-0.14 and There were a series of reports regarding the comparison of Ophiopogonis Radix from different regions. Generally, it was considered that CMD contained higher levels of steroidal saponins and ZMD had higher contents of homoisoflavonoids [31][32][33], but there were few reports on the samples from different growth years [28]. Our study showed that the growth year was also an important factor on the constituents of Ophiopogonis Radix.…”

Section: Comparison Of Ophiopogonis Radix From Different Regions And/...mentioning

confidence: 57%

“…Our study showed that the growth year was also an important factor on the constituents of Ophiopogonis Radix. For example, the content of ophiopogonin D, a main saponin of Ophiopogonis Radix, was reported to be much higher in CMD than that in ZMD [32,33], but actually it was high in one-and two-year-old ZMD, but low in three-year-old ZMD. Therefore, the differences between CMD and ZMD were not only from their different producing areas but also from their different growth years.…”

Section: Comparison Of Ophiopogonis Radix From Different Regions And/...mentioning

confidence: 99%

Rapid Screening of Lipase Inhibitors from Ophiopogonis Radix Using High-Performance Thin Layer Chromatography by Two Step Gradient Elution Combined with Bioautographic Method

et al. 2022

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In this study, a high-performance thin layer chromatography (HPTLC) method by two step gradient elution with two mobile phases was developed for the simultaneous analysis of seven constituents in Ophiopogonis Radix. The chromatography was performed on silica gel 60 F254 plate with dichloromethane-methanol-ethyl acetate-water (70:25:12:3, v/v/v/v) and dichloromethane-methanol (300:1, v/v) as the mobile phase for two step gradient elution. Then, the HPTLC profiles were observed after derivatization with 10% sulfuric acid in ethanol solution. The obtained HPTLC images were further analyzed by chemometric approaches and the samples could be clustered based on regions and/or growth years, which were two important factors affecting the constituents in Ophiopogonis Radix. Furthermore, five compounds including ophiopogonin D, ophiopojaponin C, ophiopogonin D’, ophiopogonin C’ and methylophiopogonanone B were screened as potential lipase inhibitors from Ophiopogonis Radix by the HPTLC-bioautographic method. The binding modes and interactions between the five compounds and lipase were further explored by molecular docking analysis. The developed HPTLC method could be used for quality control of Ophiopogonis Radix and screening of the potential lipase inhibitors.

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“…Then, differential compounds were filtered with ANOVE p-value (p ≤ 0.05), minimum coefficient of variation (the value ≥ 2), and max fold change (the value ≥ 2). Finally, the principal component analysis (PCA) and orthogonal partial least squared discriminant analysis (OPLS-DA) were performed by EZinfo software 3.0 (Version 3.0; Waters Co., Milford, MA) and SIMCA-P software (Version 14.1; Umetrics, Umea, Sweden) (Lyu et al, 2020).…”

Section: Multivariate Statistical Analysismentioning

confidence: 99%

A Practical Protocol for a Comprehensive Evaluation of Sulfur Fumigation of Trichosanthis Radix Based on Both Non-Targeted and Widely Targeted Metabolomics

Kang

Yang

et al. 2020

Front. Plant Sci.

Self Cite

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Trichosanthis Radix (TR) is one of the most severely sulfur-fumigated herbs in the market, whose transformation mechanism of chemical compositions and sulfur-fumigation markers of TR have not been clarified. To excavate characteristic sulfur-fumigation markers of TR samples, this study brings up a practical protocol using both ultraperformance liquid chromatography/quadrupole time-of-flight-mass spectrum (UPLC-ESI-QTOF-MS/MS)-based non-targeted metabolomics and ultra-performance liquid chromatography/electrospray ionization/quadrupole multiple-stage linear ion-trap mass spectrum (UPLC-ESI-QTRAP-MS/MS)-based widely targeted metabolomics. The results of study demonstrated that five characteristic markers are sulfur-containing components, which were identified as p-Hydroxybenzyl hydrogen sulfite, cucurbitacin D sulfite I, cucurbitacin D sulfite II, cucurbitacin B sulfite I, and cucurbitacin B sulfite II, respectively. Additionally, cucurbitacin B and D were also filtered and identified as the characteristic sulfur-fumigation markers. Meanwhile, the different sulfur-fumigation extent of TR samples was tested by chemical transformations analysis and sulfur dioxide residues test. Further, 58.16% (139 of 239) of the differential metabolites content significantly reduced in sulfur-fumigated TR samples. Besides, 20 kinds of non-sulfur marker metabolites were tested to evaluate the quality of TR samples before and after sulfur fumigation, predominantly including phenolic acids, amino acids, lipids and nucleotides. Taking TR as an example, this work provides a comprehensive practical protocol for the quality supervision of sulfur-fumigation herbs.

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Structural characterization and discrimination of Ophiopogon japonicas (Liliaceae) from different geographical origins based on metabolite profiling analysis

Cited by 20 publications

References 22 publications

Lipid Compositions and Geographical Discrimination of 94 Geographically Authentic Wheat Samples Based on UPLC-MS with Non-Targeted Lipidomic Approach

Lipid Compositions and Geographical Discrimination of 94 Geographically Authentic Wheat Samples Based on UPLC-MS with Non-Targeted Lipidomic Approach

Rapid Screening of Lipase Inhibitors from Ophiopogonis Radix Using High-Performance Thin Layer Chromatography by Two Step Gradient Elution Combined with Bioautographic Method

A Practical Protocol for a Comprehensive Evaluation of Sulfur Fumigation of Trichosanthis Radix Based on Both Non-Targeted and Widely Targeted Metabolomics

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