Structural Characterization and Biological Activities of a Novel Polysaccharide from Cultured<i>Cordyceps militaris</i>and Its Sulfated Derivative

Jing, Yongshuai; Zhu, Jianhua; Liu, Ting; Bi, Sixue; Hu, Xianjing; Chen, Zhiyan; Song, Liyan; Lv, Wenjie; Yu, Rongmin

doi:10.1021/jf505915t

Cited by 97 publications

(46 citation statements)

References 37 publications

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“…As shown in Fig. 3b and c, the COSY, TOCSY and HSQC spectra of UFP-2 revealed three distinct correlation signals in the anomeric region at 5.09 ppm/99.46 ppm, 5.02 ppm/99.46 ppm and 4.89 ppm/100.37 ppm, which can be assigned to H-1 of GalA, Rha and Gal respectively based on the previous studies 43, 44 . All chemical-shifts assignments were shown in Table 3.…”

Section: Resultssupporting

confidence: 56%

Fast preparation of RG-I enriched ultra-low molecular weight pectin by an ultrasound accelerated Fenton process

Zhi

Chen

et al. 2017

Sci Rep

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Pectin, a natural polysaccharide found in the cell wall of most higher plant such as citrus, has drawn much attention due to its potential beneficial role in facilitating the treatment of many diseases like cancer, hyper cholesterol and diabetes. However, the broad application of pectin faces great limitations as the large molecular size of pectin severely prevents its bioavailability in vivo. In this study, we report an effective and highly convenient approach to degrade natural pectin into lower molecular pectin. By combining ultrasound with Fenton system (US-Fenton), we show that ultrasound synergistically enhances the efficiency of Fenton reaction to degrade pectin into 5.5 kDa within only 35 minutes. Importantly, RG-I domain, the most effective portion of natural pectin, was well preserved and highly enriched. In addition, the antioxidant activities of US-Fenton-treated pectin was significantly elevated. The mechanism of this novel observation was further investigated through the multiple structural analyses including HPLC, IR and NMR. Taken together, we present a novel and convenient approach to generate ultra-low molecular weight pectin with high efficiency and higher bioactivity. We expect our approach will have broader applications in improving the bioavailability and bioactivity of other polysaccharide-based natural compounds.

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Section: Resultssupporting

confidence: 56%

Fast preparation of RG-I enriched ultra-low molecular weight pectin by an ultrasound accelerated Fenton process

Zhi

Chen

et al. 2017

Sci Rep

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“…The ferric reducing power of CPP was evaluated with the reported method [ 18 ] with minor modifications. The CPP samples were dissolved in distilled water to obtain different concentrations (1, 2, 3, 4, 5 and 6 mg/mL).…”

Section: Methodsmentioning

confidence: 99%

Extraction optimization and influences of drying methods on antioxidant activities of polysaccharide from cup plant (Silphium perfoliatum L.)

Shang¹,

Zhou²,

Ran³

et al. 2017

PLoS ONE

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Response surface methodology (RSM) was used to investigate the extraction condition of polysaccharide from cup plant (Silphium perfoliatum L.) (named CPP). Water to raw material ratio (10–30 mL/g), extraction time (40–80 min) and extraction temperature (60–100°C) were set as the 3 independent variables, and their effects on the extraction yield of CPP were measured. In addition, the effects of drying methods including hot air drying (HD), vacuum drying (VD) and freeze drying (FD) on the antioxidant activities of CPP were evaluated. The results showed that the optimal condition to extract CPP was: water to raw material ratio (15 mL/g), extraction time (61 min), and extraction temperature (97°C), a maximum CPP yield of 6.49% was obtained under this condition. CPP drying with FD method showed stronger reducing power (0.943 at 6 mg/mL) and radical scavenging capacities against DPPH radical (75.71% at 1.2 mg/mL) and ABTS radical (98.06 at 1.6 mg/mL) than CPP drying with HD and VD methods. Therefore, freeze drying served as a good method for keeping the antioxidant activities of polysaccharide from cup plant. The polysaccharide from cup plant has potential to use as a natural antioxidant.

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“…After the media were removed, 150 μL of DMSO was added to each well to dissolve the cellular crystalline deposits and the absorbance was determined at 570 nm. The inhibition rate (I %) was calculated according to the formula below [ 18 ].…”

Section: Methodsmentioning

confidence: 99%

Extraction Optimization, Characterization and Bioactivities of a Major Polysaccharide from Sargassum thunbergii

et al. 2015

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Sargassum thunbergii is a kind of natural edible algae. STP (S. thunbergii polysaccharides) was considered as the main bioactive compounds in S. thunbergii. To obtain the optimal processing conditions for maximum total sugar yield, single factor investigation and response surface methodology (RSM) were employed. The optimal processing conditions were as follows: liquid to solid ratio 120 mL/g, extraction time 210 min, extraction temperature 97°C. The experimental yield 7.53% under optimized conditions was closely agreed with the predicted yield 7.85% of the model. The major polysaccharide fraction from S. thunbergii (named STP-II) was purified by DEAE-Sepharose CL-6B column chromatography. High-performance size-exclusion chromatography (HPSEC), gas chromatography (GC) and high-performance liquid chromatography (HPLC) were used to identify its characterizations, and in vitro antioxidant assays and cytotoxicity assays were used to research its bioactivities. The purified fraction STP-II (63.75%) was a single peak in HPSEC with Sugar KS-804 column, had a molecular weight of 550KD, and comprised mainly of fucose, xylose, galactose, glucose and glucuronic acid. STP-II had higher scavenging activities on hydroxyl radical (76.72% at 0.7 mg/mL) and superoxide radical (95.17% at 2 mg/mL) than Vitamin C (Vc). STP-II also exhibited the capability of anti-proliferation in Caco-2 cells. STP-II possessed good antioxidant and inhibitory activity against human colon cancer Caco-2 cells in vitro and could be explored as novel natural functional food.

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Structural Characterization and Biological Activities of a Novel Polysaccharide from CulturedCordyceps militarisand Its Sulfated Derivative

Cited by 97 publications

References 37 publications

Fast preparation of RG-I enriched ultra-low molecular weight pectin by an ultrasound accelerated Fenton process

Fast preparation of RG-I enriched ultra-low molecular weight pectin by an ultrasound accelerated Fenton process

Extraction optimization and influences of drying methods on antioxidant activities of polysaccharide from cup plant (Silphium perfoliatum L.)

Extraction Optimization, Characterization and Bioactivities of a Major Polysaccharide from Sargassum thunbergii

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