Structural characterization and antioxidant activity of polysaccharides extracted from Chinese yam by a cellulase-assisted method

Liu, Xuexia; Gu, Lingbiao; Zhang, Guang-Jie; Liu, Hua‐Min; Zhang, Yating; Zhang, Kunpeng

doi:10.1016/j.procbio.2022.06.023

Cited by 24 publications

(6 citation statements)

References 44 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Indeed, several studies reported that the antioxidant activities of polysaccharides extracted from numerous medicinal plants were significant and that those carbohydrates may be used as potential antioxidants [ 15 , 35 ]. Similar data were recently reported [ 45 , 47 ]. Antioxidant capacity showed a significant and positive correlation with polyphenols contents, as most of these constituents are proven scavengers of free radicals [ 48 ].…”

Section: Discussionsupporting

confidence: 92%

Polysaccharides from South Tunisian Moringa alterniflora Leaves: Characterization, Cytotoxicity, Antioxidant Activity, and Laser Burn Wound Healing in Rats

Aydi

Khadher

et al. 2023

Plants

View full text Add to dashboard Cite

Phytochemical properties have recently increased the popularity of plant polysaccharides as wound dressing materials. This work aims at studying the structural characteristics of polysaccharides extracted from Moringa leaves (Moringa Leaves Water Soluble Polysaccharide: MLWSP), and its antioxidant activities, cytotoxic effects, and laser burn wound healing effects in rats. This MLWSP was structurally characterized. Results showed 175.21 KDa and 18.6%, respectively, for the molecular weight and the yield of the novel extracted polysaccharide. It is a hetero-polysaccharide containing arabinose, rhamnose, and galactose. XRD suggested a semi-crystalline structure of the studied polymer and FT-IR results revealed a typical polysaccharide structure. It is composed of 50 to 500 µm rocky-shaped units with rough surfaces and it was found to inhibit the proliferation of the human colon (HCT-116) (IC50 = 36 ± 2.5 µg/mL), breast (MCF-7) (IC50 = 48 ± 3.2), and ovary cancers (IC50 = 24 ± 8.1). The MLWSP showed significant antioxidant effects compared to Trolox (CI50 = 0.001 mg/g). Moreover, promising wound healing results were displayed. The effect of MLWSP hydrogel application on laser burn injuries stimulated wound contraction, re-epithelization, and remodeling phases 8 days after treatment. The wound healing potential of MLWSP may be due to its significant antioxidant activity and/or the huge amount of monosaccharide molecules.

show abstract

Section: Discussionsupporting

confidence: 92%

Polysaccharides from South Tunisian Moringa alterniflora Leaves: Characterization, Cytotoxicity, Antioxidant Activity, and Laser Burn Wound Healing in Rats

Aydi

Khadher

et al. 2023

Plants

View full text Add to dashboard Cite

show abstract

“…Figure 5 shows the NMR spectra of the HSQC of RGP−1−A and RGP−2−A. The signals at 4.99/107.09 ppm (RGP−1−A) and 4.99/107.09 ppm (RGP−2−A) were designated as α-L-Ara based on relevant studies and literature [ 63 ]. The signal at 4.55/104.08 ppm (RGP−1−A) was designated as β-D-Gal [ 64 ].…”

Section: Resultsmentioning

confidence: 99%

Structural Characteristics of Rehmannia glutinosa Polysaccharides Treated Using Different Decolorization Processes and Their Antioxidant Effects in Intestinal Epithelial Cells

Ren

Gao

et al. 2022

Foods

View full text Add to dashboard Cite

Polysaccharide decolorization is a key determinant of polysaccharide structure. In this study, two purified Rehmannia glutinosa polysaccharides, RGP−1−A and RGP−2−A, were obtained after decolorization using the AB-8 macroporous resin and H2O2, respectively. RGP−1−A (molecular weight (Mw) = 18,964 Da) and RGP−2−A (Mw = 3305 Da) were acidic and neutral heteropolysaccharides, respectively, and were both polycrystalline in structure. FTIR analysis revealed that RGP−1−A was a sulfate polysaccharide, while RGP−2−A had no sulfate group. Experiments on IPEC-1 cells showed that RGPs alleviated oxidative stress by regulating the Nrf2/Keap1 pathway. These findings were confirmed by the upregulation of Nrf2, NQO1, and HO-1; the subsequent increase in the levels of antioxidant indicators (SOD, LDH, CAT, and MDA); and the restoration of mitochondrial membrane potential. Overall, the antioxidant capacity of RGP−1−A was significantly higher than that of RGP−2−A. These results suggest that RGPs may be a potential natural antioxidant and could be developed into functional foods.

show abstract

“…In addition, the positive effect of the galactose content in polysaccharides on the AOA was reported [ 4 ], which is consistent with the data on the monosaccharide composition: the highest galactose content is observed in sample 100-4. According to [ 48 , 49 ], the polysaccharides with a relatively low molecular weight show a stronger ability to absorb DPPH radicals. It is important to note, however, that, although sample 70-4 has the highest molecular weight (33,142 g/mol), its AOA is similar to that of sample 100-4.…”

Section: Resultsmentioning

confidence: 99%

Composition and Structure of Aspen (Pópulus trémula) Hemicelluloses Obtained by Oxidative Delignification

et al. 2022

View full text Add to dashboard Cite

In this study, hemicelluloses of aspen wood (Pópulus trémula) were obtained by oxidative delignification in an acetic acid‒water‒hydrogen peroxide medium at temperatures of 70‒100 °C and a process time of 1‒4 h. The maximum polysaccharide yield of up to 9.68 wt% was reported. The composition and structure of the hemicelluloses were studied using a complex of physicochemical methods: gas and gel permeation chromatography, Fourier-transform infrared spectroscopy, 2D nuclear magnetic resonance spectroscopy, and thermogravimetric analysis. The xylose, mannose, galactose, and glucose monomer units were identified in the hemicelluloses by gas chromatography. The weight average molecular weight Mw of the products determined by gel permeation chromatography was found to range within 8932‒33,142 g/mol. The reported Fourier-transform spectra of the hemicelluloses contain all the bands characteristic of heteropolysaccharides; a weak lignin absorption signal in the spectra at 1500‒1510 cm−1 is attributed to a minor content of phenolic fragments in the structure of the obtained hemicelluloses. The use of thermogravimetric analysis established that the hemicelluloses isolated from aspen wood are resistant against heating to temperatures of up to 90–100 °C and, upon further heating up to 400 °C, start destructing at an increasing rate. The antioxidant activity of the hemicelluloses was examined using the compounds that mimic free radicals (1,1-diphenyl-2-picrylhydrazyl) and hydroxyl radicals (salicylic acid). It was found that the activity of all polysaccharides in neutralizing DPPH and hydroxyl radicals is lower than the absorption capacity of vitamin C at all the tested concentrations (0.5, 2, and 5 mg/mL) and attains 81.7 and 82.9%, respectively.

show abstract

Structural characterization and antioxidant activity of polysaccharides extracted from Chinese yam by a cellulase-assisted method

Cited by 24 publications

References 44 publications

Polysaccharides from South Tunisian Moringa alterniflora Leaves: Characterization, Cytotoxicity, Antioxidant Activity, and Laser Burn Wound Healing in Rats

Polysaccharides from South Tunisian Moringa alterniflora Leaves: Characterization, Cytotoxicity, Antioxidant Activity, and Laser Burn Wound Healing in Rats

Structural Characteristics of Rehmannia glutinosa Polysaccharides Treated Using Different Decolorization Processes and Their Antioxidant Effects in Intestinal Epithelial Cells

Composition and Structure of Aspen (Pópulus trémula) Hemicelluloses Obtained by Oxidative Delignification

Contact Info

Product

Resources

About