Structural characterization and anti-oxidation activity evaluation of pectin from Lonicera japonica Thunb.

Abstract

Pectins are nutrient components of plants and are widely used in the food industry. In this study, one major pectin fraction (WLJP-A0.2b) with Mw of 40.6 kDa was purified from Lonicera japonica Thunb. The structural feature and antioxidant activity of it was investigated. Monosaccharide composition, Fourier transform infrared (FT-IR) spectra, enzymatic hydrolysis, and nuclear magnetic resonance (NMR) spectra analysis indicated that WLJP-A0.2b consisted of rhamnogalacturonan I (RG-I), rhamnogalacturonan II (RG-II), and homogalacturonan (HG) domains, with mass ratio of 0.4:1.0:2.1. The RG-I domain contained highly branched α-L-1,5-arabinan, β-D-1,4-galactan and type II arabinogalactan (AG-II) side chains. The HG domain was released in the form of un-esterified and partly methyl-esterified and/or acetyl-esterified oligogalacturonides with degree of polymerization 1–8 after degradation by endo-polygalacturonase. Radical scavenging assays indicated that WLJP-A0.2b exhibited antioxidant activity through the synergistic effects of different pectin domains. Oligogalacturonides, especially de-esterified oligogalacturonides, showed better antioxidant activities than RG-II and RG-I domains. Moreover, de-esterified oligogalacturonides remarkably reduced H2O2-induced reactive oxygen species production in HEK-293T cells. These results provide useful information for screening of natural antioxidants from Lonicera japonica Thunb. and application of pectin in functional food field.

Characterization and anti-aging activities of polysaccharide from Rana dybowskii Guenther

“…Polysaccharide content was determined by the phenol-sulfuric acid method using glucose as a standard ( Yang Y. et al, 2023 ). Protein and uronic acid were determined by the Bradford method and the m-hydroxybiphenyl method, respectively, bovine serum albumin and D-galacturonic acid were used as standards, respectively ( Qi et al, 2022 ; Zhang et al, 2023 ). The sulfate content was determined by the barium chloride turbidimetric method using potassium sulfate as the standard ( Liu et al, 2016 ).…”

Recent advances in the production of oligogalacturonides and their biological properties

“…Several approaches have been described in the literature for pectin depolymerization, including physical treatments, such as electron beam irradiation, 29 free-radical hydrolysis, 38 physico-chemical processes, 25,37 enzymatic hydrolysis either in submerged systems 8,11,12,15,21–23,35 or with immobilized enzymes 24,31 and even microbial fermentation using wild 10 or genetically modified microorganisms. 32,34 In addition, combined technologies such as enzymatic hydrolysis and high hydrostatic pressure 7 or enzymatic hydrolysis and chemical de-esterification 14 were also employed for the production of di- and tri-galacturonides and mixtures of OGalA with DP1–8, respectively.…”

“…During the last few years, several raw materials (mainly fruits, agroindustrial byproducts and pectins derived from them) have been used for production of OGalA, including haw, 12,19–22,31 apple pectin, 7,9,10 artichoke, 13 mango peel wastes, 24 lemon peels, 27 mandarin peels, 11,32 finger citron pomace, 15 pomelo peels, 29 sugar beet pulp, 33,34 citrus pectin, 8,23,25 sunflower plate pectin 35,36 and Lonicera japonica Thunb. 14 With the same purpose, commercial polygalacturonic acid (PGalA) was also tested. 25,37 Table 1 summarizes the relevant information provided in all the reviewed studies about OGalA production, including raw materials, treatments and refining strategies as well as product characteristics.…”

“…As stated before, to advance in the knowledge of the structure–function interrelationships, well-isolated and characterized products need to be used in laboratory assays. In an effort to delve deeper into this issue, the isolation of OGalA from streams containing mixtures of oligomers has been assessed by several techniques, among which membrane filtration 15,22,25,31 and/or chromatographic separation 12,14,20,21,31,33,44 are the most used. However, other types of refining operations such as ion exchange 7 and ethanol precipitation 25 were also tested.…”

“…In this context, during the last decade, some in vitro and in vivo assays have been performed using well isolated and characterized oligogalacturonides, demonstrating a variety of interesting biological properties, including anti-inflammatory, 6,7 anticancer, 8–11 antioxidant, 10,12–15 antilipidemic, 12,16–19 antidiabetic, 20 antiobesity, 16,17,20,21 and antibacterial, 22–24 together with prebiotic effects. 25–29 Moreover, although anti-inflammatory and anticancer effects have also been reported for pectin, 30 its poor solubility together with its difficultly in penetrating the body barrier to reach cells affect its biological functions.…”