We performed two experiments to examine how temperature and nutrients interact to control dinitrogen (N 2 ) fixation, chlorophyll a (Chl a) biomass, and community composition of periphyton in subalpine oligotrophic streams in the Sawtooth Mountains of Idaho. We grew periphyton on nutrient-diffusing substrata (NDS) in a cold lake inlet (7uC) and a warm lake outlet (18uC). We then switched substrata between the two stream sites to test the effect of incubation temperature on N 2 -fixation rates. Periphyton on substrata grown at both sites exhibited greater N 2 -fixation rates when incubated in the warm outlet, which indicates physiologic temperature control. Periphyton on P-enriched NDS grown in the warm outlet had the greatest N 2 -fixation rates, largest Chl a biomass, and largest percentage of N 2 -fixing taxa of any treatment, which indicates that temperature and P interact to influence the community. In the second experiment, colonized rocks and uncolonized NDS were placed in cold (13uC) and warm (18uC) mesocosms. Within 2 days, warm temperature stimulated N 2 fixation by the rock periphyton community two times above cold temperatures, which indicates physiologic temperature control. After 45 days, warm temperatures and P enrichment led to Anabaena sp. in the periphyton community and the greatest rates of N 2 fixation observed in the experiment, which also indicates temperature and nutrient control at the community level. This study indicates that N 2 fixation and periphyton community composition in oligotrophic streams are controlled by both temperature and P supply, with temperature modulating the response to P.