Structural Characteristics and Functional Analysis of Gut Microbiome in Patients with Osteoarthritis

Abstract

ObjectiveRecent studies illustrated that changes of gut microbiome in human body may be related to the pathogenesis of osteoarthritis (OA). We aimed to determine the difference of gut microbiome between patients with osteoarthritis and normal people.MethodsThe samples were collected from 56 patients with osteoarthritis (34 females) and 52 healthy subjects (28 females). High-throughput sequencing of 16S rRNA gene was used to analyze the classification and composition of gut microbiome in OA patients, and then we used Linear discriminant analysis Effect Size (LEfSe) to find out the main differential bacteria and biomarkers in OA patients. At last, PICRUSt was applied to predict the genomic function of gut microbiome in OA patients, and we explored its mechanism in the development of the OA.ResultsThe association between gut microbiome and osteoarthritis has not been demonstrated in detail. Herein, we investigated the gut microbiome of OA patients and its relationship with the disease to evaluate the potential of the gut microbiome in the diagnosis and treatment of osteoarthritis. And we identified that there are significant differences in the β diversity of the gut microbial composition between the control group and OA patients (p＜0.05, respectively), and Bifidobacterium, Faecalibacterium and Alistipes have the potential to become biomarkers of osteoarthritis. Besides, the genomes of gut microbiome in OA patients are enriched in metabolic pathways and lipopolysaccharide biosynthesis (p=0.049, p=0.005), but in signal pathways related to apoptosis, the degree of enrichment was significantly higher than that of healthy people (p=0.006).ConclusionOur research identified that the community composition of gut microbiome in OA patients was different from that in normal groups and explored the role of gut microbiome in the development of osteoarthritis, which may provide a new theoretical basis for the study of osteoarthritis.

Microbiome and osteoarthritis: New insights from animal and human studies

“…Individuals with OA had a higher abundance of Bacteroidia at the class level; Bacteroidales at the order level (Table 3). When the gut microbiome of OA patients was compared with that of healthy controls, greater abundance of Bifidobactericeae , Bifidobacterium , and Alistipes was seen 81 . These findings are similar to the gut microbiome profile of OA patients described by Lee et al 80 …”

Comparative Analysis of the Bioactive Compounds in Chicken Cartilage: Protective Effects of Chondroitin Sulfate and Type II Collagen Peptides Against Osteoarthritis Involve Gut Microbiota

“…This was consistent with the result of LEfSe that Lactobacillus was the biomarker of OA. Xie et al found that the genus Lactobacillus abundance and family Lactobacillaceae abundance of OA people were significantly higher than those of healthy people ( 50 ). Liu et al indicated that the fecal microbiota of patients with rheumatoid arthritis showed significantly more Lactobacillus (10.62 ± 1.72 copies/g) than that of the control group (8.93 ± 1.60 copies/g) ( 51 ).…”