Structural characterisation and immunomodulatory activity of exopolysaccharides from liquid fermentation of Monascus purpureus (Hong Qu)

Wang, Nifei; Jia, Gege; Wang, Changlu; Chen, Mianhua; Xie, Fengjiao; Nepovinnykh, N.V.; Goff, H. Douglas; Guo, Qingbin

doi:10.1016/j.foodhyd.2019.105636

Cited by 41 publications

(23 citation statements)

References 36 publications

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“…1D). 1 The peak at 3394.11 cm −1 was attributed to the O-H stretching vibration in the sugar molecule, while that at 2978.55 cm −1 was caused by the C-H stretching vibration from the CH 2 and CH 3 groups. The band at 1695.18 cm −1 was related to the deformation vibration of the hydroxyl groups, while the absorption peak at 1471.69 cm −1 represented the CvC stretching vibrations.…”

Section: Uv-visible and Ft-ir Spectral Analysesmentioning

confidence: 98%

“…Additionally, an EPS from M. purpureus (Hong Qu) significantly promoted the secretion of cytokines such as TNF-α and IL-10, and moreover, increased the expression levels of relevant mRNAs based on the macrophage model. 1 Numerous studies have demonstrated that the functional effects of polysaccharides are closely linked to their physicochemical properties and structures, such as molecular weight (M w ), monosaccharide composition, monosaccharide composition ratio, and chain spatial structure. 4 As a result, an increasing number of researchers are using various physical, chemical, and biological techniques to modify the structure of polysaccharides to further enhance their biological activity.…”

Section: Reported That Mildly Alkaline Extraction Conditions [Extraction Time Of 23 H Materialsmentioning

confidence: 99%

“…Monascus purpureus is a common medicinal and edible fungus, and contains several beneficial secondary metabolites, including monacolin K, γ-aminobutyric acid, and Monascus pigments, which possess medicinal properties, such as hypoglycemic, antioxidant, anti-inflammatory, and anti-tumor properties. 1 Among the secondary metabolites, M. purpureus polysaccharides have recently attracted considerable research attention. Jia et al 2 systematically compared the structures and antioxidant effects of different exopolysaccharides (EPS) from M. purpureus.…”

Section: Introductionmentioning

confidence: 99%

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Role of genistein on the yield, structure and immunomodulatory activity of Monascus exopolysaccharides

et al. 2022

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Section: Uv-visible and Ft-ir Spectral Analysesmentioning

confidence: 98%

Section: Reported That Mildly Alkaline Extraction Conditions [Extraction Time Of 23 H Materialsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Role of genistein on the yield, structure and immunomodulatory activity of Monascus exopolysaccharides

et al. 2022

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“…Indeed metagenomic sequencing has been widely applied for the identification of functional microorganisms in fermented foods, including wine (Liu et al, 2019 ), vinegar (Wu L. et al, 2017 ), cheese (Escobar-Zepeda et al, 2016 ), pickles (Jung et al, 2011 ), cocoa beans (Agyirifo et al, 2019 ), and fermented sausages (Ferrocino et al, 2017 ). Furthermore, metabolomics analysis based on gas chromatography (GC), mass spectrometry (MS), high-performance liquid chromatography (HPLC), and nuclear magnetic resonance revealed the metabolite profiles of jiuqu (Tang et al, 2019 ; Wang N. et al, 2019 ) and Baijiu (Huo et al, 2020 ; Jia et al, 2020 ; Liu and Miao, 2020 ). However, former studies on Xiaoqu have primarily focused on microorganisms and volatile flavors (Liu and Sun, 2018 ; Tang et al, 2019 ; Chen C. et al, 2020 ), whereas the correlation between microorganisms and non-volatile metabolites requires further investigation.…”

Section: Introductionmentioning

confidence: 99%

Integrative Metagenomics–Metabolomics for Analyzing the Relationship Between Microorganisms and Non-volatile Profiles of Traditional Xiaoqu

Zhao

et al. 2021

Front. Microbiol.

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Xiaoqu, one of three traditional jiuqu in China, is a saccharifying and fermenting agent used in Xiaoqu jiu brewing, with different ingredient compositions and preparation techniques used in various regions. The yield and quality of Xiaoqu jiu are significantly affected by the metabolites and microbiota of Xiaoqu; however, the associated relationship remains poorly understood. This study aimed to analyze this relationship in three typical traditional Xiaoqu from the Guizhou province in China. The non-volatile metabolites of Xiaoqu were detected using gas chromatography time-of-flight mass spectrometry, whereas the classification and metabolic potential of the microbiota were investigated using metagenomic sequencing. Results show that Firmicutes, Proteobacteria, and Actinobacteria represent the dominant bacterial phyla, with Lactobacillus, Bacillus, Acinetobacter, Leuconostoc, and Weissella found to be the dominant bacterial genera. Meanwhile, Ascomycota, Mucoromycota, and Basidiomycota are the dominant fungal phyla with Aspergillus, Saccharomyces, Pichia, Rhizopus, and Phycomyces being the predominant fungal genera. Functional annotation of the microbiota revealed a major association with metabolism of carbohydrates, cofactors, and vitamins, as well as amino acids. A total of 39 significantly different metabolites (SDMs) were identified that are involved in 47 metabolic pathways, primarily that of starch and sucrose; glycine, serine, and threonine; glyoxylate and dicarboxylate; pyruvate; as well as biosynthesis of pantothenate and CoA. Further, based on Spearman's correlation analysis, Aspergillus, Saccharomyces, Lactobacillus, Acetobacter, Weissella, Pantoea, Desmospora, and Bacillus are closely correlated with production of physicochemical indexes and SDMs. Moreover, the metabolic network generated for the breakdown of substrates and formation of SDMs in Xiaoqu was found to primarily center on the metabolism of carbohydrates and the tricarboxylic acid cycle. These results provide insights into the functional microorganisms and metabolic patterns present in traditional Guizhou Xiaoqu and might guide researchers in the production of stable and efficient Xiaoqu in the future.

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“…The purified LSEP (30 mg) was dissolved in D 2 O (99%, 1.0 mL) [68]. A nuclear magnetic resonance (NMR) spectrometer (Bruker Advance 400 MHz) was used to record 1 H and 13 C NMR spectra at 25 • C. X-ray Diffraction Analysis X-ray diffraction (XRD) analysis of LSEP was performed on an X-ray powder diffractometer (Smart Lab, Rigaku, The Woodlands, TX, USA).…”

Section: Nuclear Magnetic Resonance Analysismentioning

confidence: 99%

Physicochemical Characterization of an Exopolysaccharide Produced by Lipomyces sp. and Investigation of Rheological and Interfacial Behavior

Guo

Chen

et al. 2021

Gels

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The present study aimed to evaluate the rheological and interfacial behaviors of a novel microbial exopolysaccharide fermented by L. starkeyi (LSEP). The structure of LSEP was measured by LC-MS, 1H and 13C NMR spectra, and FT-IR. Results showed that the monosaccharide composition of LSEP was D-mannose (8.53%), D-glucose (79.25%), D-galactose (7.15%), and L-arabinose (5.07%); there existed the anomeric proton of α-configuration and the anomeric carbon of α- and β-configuration; there appeared the characteristic absorption peak of the phosphate ester bond. The molecular weight of LSEP was 401.8 kDa. The water holding capacity (WHC, 2.10 g/g) and oil holding capacity (OHC, 12.89 g/g) were also evaluated. The results of rheological properties showed that the aqueous solution of LSEP was a non-Newtonian fluid, exhibiting the shear-thinning characteristics. The adsorption of LSEP can reduce the interfacial tension (11.64 mN/m) well and form an elastic interface layer at the MCT–water interface. Such functional properties make LSEP a good candidate for use as thickener, gelling agent, and emulsifier to form long-term emulsions for food, pharmaceutical, and cosmetic products.

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Structural characterisation and immunomodulatory activity of exopolysaccharides from liquid fermentation of Monascus purpureus (Hong Qu)

Cited by 41 publications

References 36 publications

Role of genistein on the yield, structure and immunomodulatory activity of Monascus exopolysaccharides

Role of genistein on the yield, structure and immunomodulatory activity of Monascus exopolysaccharides

Integrative Metagenomics–Metabolomics for Analyzing the Relationship Between Microorganisms and Non-volatile Profiles of Traditional Xiaoqu

Physicochemical Characterization of an Exopolysaccharide Produced by Lipomyces sp. and Investigation of Rheological and Interfacial Behavior

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