Structural changes of water caused by non-electrolytes: Volumetric and compressibility approach for urea-like analogues

Insight into Interactions of l-Arginine/l-Histidine with Drug Betaine Hydrochloride in Aqueous Medium at Different Temperatures by using Physicochemical Methods

“…According to the intuition, bonds loosen, and intermolecular distances increase, as the temperature increases, leading to higher compressibility of solvent. Similar trends are observed in literature [24,25].…”

“…In the original tetrahedral structure of water, one position is taken by ions and only three water molecules can be hydrogen bonded which are further counted as hydration number in its primary shell. Therefore, water coordination number for these bonded ions will be less than those for the pure water [24,41].…”

Monitoring of lysozyme thermal denaturation by volumetric measurements and nanoDSF technique in the presence of N-butylurea

“…It is known that dissolved low molecular weight substances can change the structure of water in many different ways [ 10 ]. It has been postulated that the properties of the protein will be different in such an altered environment [ 11 ].…”

“…It has been suggested that the big increase in the thermal volume Δ v T could be the missing factor not taken into consideration [ 18 ]. It is also possible that the hydration of the non-polar residues gives a positive contribution to the change of the volume during protein unfolding [ 10 ]. It was also questioned if the transfer studies of the non-polar compounds provide a good reflection of the protein unfolding.…”

“…N -butylurea (BU) was chosen for the research due to the unusual properties of the hydration sphere formed around this compound [ 10 ]. Despite its non-polar character, BU does not break the structure of the water but makes it more reinforced.…”