Structural changes of proteins in fish red blood cells after copper and mercury treatment

Gwoździński, Krzysztof

doi:10.1007/bf00203804

Cited by 12 publications

(6 citation statements)

References 21 publications

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“…Other studies confirm a rapid and high accumulation of dietary methylmercury in the blood (Skak & Baatrup 1993;Oliveira et al 1999) with most of the methylmercury associated with red blood cells (Riisgard & Hansen 1990;Bjerregaard et al 1999). Methylmercury is well known to affect permeability of erythrocyte membranes with haemolysis as a consequence (Gwozdzinski 1992). Similarly, in the present study, the decrease in blood Hct and increased total plasma protein in fish fed 5 mg kg )1 methylmercury and higher concentrations, indicate haemolysis.…”

Section: Organ Contamination and Sublethal Effectssupporting

confidence: 81%

“…1999). Methylmercury is well known to affect permeability of erythrocyte membranes with haemolysis as a consequence (Gwozdzinski 1992). Similarly, in the present study, the decrease in blood Hct and increased total plasma protein in fish fed 5 mg kg −1 methylmercury and higher concentrations, indicate haemolysis.…”

Section: Organ Contamination and Sublethal Effectsmentioning

confidence: 99%

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Maximum limits of organic and inorganic mercury in fish feed

Berntssen¹,

Hylland

Julshamn³

et al. 2004

Aquac Nutrition

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The relatively high levels of mercury found in fish feeds might form a fish health and food safety risk. The present study aims to establish sublethal toxic threshold levels in fish and assess feed‐fillet transfer of dietary mercury. Atlantic salmon (Salmo salar L.) parr were fed for 4 months on fish meal‐based diets supplemented with mercuric chloride (0, 0.1, 1, 10 or 100 mg Hg kg−1 dry weight (DW)) or methylmercuric chloride (0, 0.1, 0.5, 5 or 10 mg MeHg kg−1 DW). At the end of the experiment, dietary inorganic mercury mainly accumulated in intestine (80% of body burden) and assimilation was low (6%). In contrast, methylmercury readily accumulated in internal organs and muscle (80% of body burden) and had a relatively high assimilation (23%). Highest accumulation of dietary inorganic mercury was observed in the gut and kidney. Fish fed 10 mg Hg kg−1 had an early (after 2 months) significant increase in renal metallothionein (MT) level and intestinal cell proliferation, followed by intestinal pathological conditions after 4 months of exposure. At 100 mg Hg kg−1, intestinal and renal function were reduced as seen from the significantly reduced protein and glycogen digestibility and increased plasma creatinine levels. For dietary methylmercury (MeHg), highest accumulation was found in blood and muscle. Intestinal cell proliferation and liver MT significantly increased at 5 mg MeHg kg−1 after 2 months of exposure. At the end of the experiment, blood haematology was significantly affected in fish fed 5 mg MeHg kg−1 and these fish exceeded the current food safety limit for mercury. Tissue MT induction and intestinal cell proliferation appeared to be useful and quantifiable early indicators of toxic mercury exposures. Based on the absence of induction of these early biological markers such as MT and cell proliferation, nonobserved effect levels (NOELs) could be set to 0.5 mg Hg kg−1 for dietary methylmercury and 1 mg Hg kg−1 for inorganic mercury. Lowest observed effect levels (LOELs) levels could be set to 5 mg kg−1 for methylmercury and 10 mg Hg kg−1 for inorganic mercury.

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Section: Organ Contamination and Sublethal Effectssupporting

confidence: 81%

Section: Organ Contamination and Sublethal Effectsmentioning

confidence: 99%

Maximum limits of organic and inorganic mercury in fish feed

Berntssen¹,

Hylland

Julshamn³

et al. 2004

Aquac Nutrition

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show abstract

“…The quenching in tryptophan fluorescence in the presence of mercuric chloride may be due to the interactions of mercury with the proteins by forming metal‐protein complexes, leading to disruption of the protein‐membrane interactions. Further, formation ‐S‐Hg‐S‐ bridge between mercury and peptides of the membrane proteins, causing exposure of its hydrophobic side toward the cytoplasmic side affecting the corresponding protein conformation . Similarly, in the case of sodium arsenite, which interacted with the sulfhydryl group of cysteine and residues of proteins, led to protein conformational changes .…”

Section: Resultsmentioning

confidence: 99%

Detection of mitochondrial dysfunction in vitro by laser‐induced autofluorescence

Raghushaker

Chandra

Chakrabarty

et al. 2019

Journal of Biophotonics

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Mitochondrion plays a significant role in a variety of biological functions. Because of their diverse character and location in the cellular systems, mitochondria commonly get exposed to various extrinsic and intrinsic cellular stresses. The present study reports a novel approach to detection of mitochondrial dysfunction based on tryptophan autofluorescence of its proteins in mouse liver, using laser-induced fluorescence (LIF) as a tool. Mitochondria, isolated from the mouse liver, were initially tested for purity and integrity using lactate dehydrogenase and succinate dehydrogenase (SDH) assays.Mitochondrial stress was induced by treating the isolated mitochondria with heavy metals at 10 and 0.01 mM for sodium arsenite and mercuric chloride, respectively. Upon treatment with the heavy metal, tryptophan autofluorescence quenching was recorded at 281 nm excitation. The functional integrity of the mitochondria treated with heavy metals was evaluated by measuring SDH and cytochrome c oxidase activities at various concentrations of mitochondria, which showed impaired activity as compared to control upto a concentration of 6.25 μg. A significant shift was also observed in the autofluorescence of proteins upto the level below 1 μg, suggesting their conformational change and hence altered structural integrity of mitochondria. Circular dichroism spectroscopy data of the mitochondrial proteins treated with heavy metals further validates their conformational change as compared to untreated control. The present study clearly shows that the LIF can be a novel detection tool to detect altered structural integrity of cellular mitochondria upon stress, and it also possesses the potentiality to combine with other interdisciplinary modalities. K E Y W O R D SANS fluorescence, heavy metals, LDH and SDH assay, tryptophan autofluorescence

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“…Fish have been reported to have erythrocyte morphological disorders when the environment is contaminated by a variety of factors, particularly organic substances such as phenol compounds (Sharma and Chadha 2021), detergents (Zeni et al 2002), tributyltin chloride (TBTC) (Tiano et al 2003), thiamethoxam (Ghaffar et al 2020), tetrabromodiphenyl ether (Bolognesi et al 2006), as well as heavy metals like Cr (Suchana et al 2021), Hg (Gwoździński 1992), Pb (Ahmed et al 2022;Hofer et al 1992;Monteiro et al 2011;Witeska 2004), Cd (Hofer et al 1992;Witeska 2004;Witeska et al 2006), Cu (Bagdonas and Vosylienė 2006;Gwoździński 1992;Witeska 2004), Zn (Bagdonas and Vosylienė 2006). Fish also exhibit a wide range of erythrocyte morphological disorders, which vary between species.…”

Section: Discussionmentioning

confidence: 99%

Morphological Alterations of Fish Erythrocytes as Their Response to Environmental Conditions

Quyet

Dung

2023

HAYATI J Biosci

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Fish bodies respond to changes in environmental conditions in various ways and degrees. Seven fish species were collected from three study locations, including two nearshore sites (Ha Tinh province and Nha Trang city, Khanh Hoa province) and one offshore site (Toc Tan island, Khanh Hoa province) to evaluate alterations in erythrocyte morphology under certain environmental conditions. Heavy metal concentrations at all study sites were analyzed and met the acceptable technical criteria for each site. Fish blood samples were taken from the tail veins, anticoagulated with heparin, and stained with Giemsa stain. The blood cell morphological study was performed using an optical microscope. The results revealed two types of morphological abnormalities of fish erythrocytes, including nuclear deformation and nuclear-matter distribution.

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Structural changes of proteins in fish red blood cells after copper and mercury treatment

Cited by 12 publications

References 21 publications

Maximum limits of organic and inorganic mercury in fish feed

Maximum limits of organic and inorganic mercury in fish feed

Detection of mitochondrial dysfunction in vitro by laser‐induced autofluorescence

Morphological Alterations of Fish Erythrocytes as Their Response to Environmental Conditions

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