Structural changes of gut microbiota in a rat non-alcoholic fatty liver disease model treated with a Chinese herbal formula

Yin, Xiaochen; Peng, Jinghua; Zhao, Liping; Yu, Yunpeng; Zhang, Xu; Liu, Ping; Feng, Qin; Hu, Yiyang; Pang, Xiaoyan

doi:10.1016/j.syapm.2012.12.009

Cited by 72 publications

(47 citation statements)

References 48 publications

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“…One of these species is Mitsuokella jalaludinii, significantly depleted after the HFS treatment. Mitsuokella jalaludinii, has been recently isolated from the rumen of cattle (Lan et al, 2002) and is a member of the Veillonellaceae family that has been previously demonstrated to be reduced in HFD-fed rats (Yin et al, 2013). In our experiment, Veillonellaceae did not statistically differ between groups but, as observed in the PCA, it was represented in a greater extent in HFS diet-fed rats.…”

Section: Discussionsupporting

confidence: 53%

Shifts in microbiota species and fermentation products in a dietary model enriched in fat and sucrose

Etxeberría

Arias

Boqué

et al. 2015

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The gastrointestinal tract harbours a "superorganism" called the gut microbiota, which is known to play a crucial role in the onset and development of diverse diseases. This internal ecosystem, far from being a static environment, could be willingly manipulated by diet and dietary components. Feeding animals with high-fat sucrose diets entails diet-induced obesity, a model which is usually used in research to mimic the obese phenotype of Western societies.The aim of the present study was to identify gut microbiota dysbiosis and associated metabolic changes produced in 5 male Wistar rats fed a high-fat sucrose (HFS) diet for six weeks and to compare it with the basal microbial composition. For this purpose, DNA extracted from faeces at baseline and after the treatment was analysed by amplification of the V4-V6 region of the 16S ribosomal DNA (rDNA) gene using 454 pyrosequencing. Short-chain fatty acids (SCFA), acetate, propionate and butyrate, were also evaluated by gas chromatography-mass spectrometry (GC-MS).At the end of the treatment, gut microbiota composition significantly differed at phylum level (Firmicutes, Bacteroidetes and Proteobacteria) and class level (Erisypelotrichi, Deltaproteobacteria, Bacteroidia and Bacilli). Interestingly, Clostridia class showed a significant decrease after the HFS-diet treatment, which correlated with visceral adipose tissue, and is likely mediated by dietary carbohydrates. Of particular interest, Clostridium cluster XIVa species were significantly reduced and changes were identified in the relative abundance of other specific bacterial species (Mitsuokella jalaludinii, Eubacterium ventriosum, Clostridium sp. FCB90-3, Prevotella nanceiensis, Clostridium fusiformis, Clostridium sp. BNL1100 and Eubacterium cylindroides) that, in some cases, showed opposite trends to their relative families.These results highlight the relevance of characterizing gut microbial population differences at species level and contribute to understand the plausible link between the 1 diet and specific gut bacterial species that are able to influence the inflammatory status, intestinal barrier function and obesity development.

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Section: Discussionsupporting

confidence: 53%

Shifts in microbiota species and fermentation products in a dietary model enriched in fat and sucrose

Etxeberría

Arias

Boqué

et al. 2015

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“…We previously observed that QHD treatment decreased the opportunistic pathogen Escherichia/Shigella and increased the SCFA producer Collinsella [15]. It seems that QHD treatment may improve the gut barrier function through other mechanisms in addition to the Treg mediated pathway identified in this study.…”

Section: Discussionmentioning

confidence: 52%

Multi-targeting therapeutic mechanisms of the Chinese herbal medicine QHD in the treatment of non-alcoholic fatty liver disease

et al. 2017

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Beneficial effects of the Chinese herbal medicine Qushi Huayu Decoction (QHD) were observed with non-alcoholic fatty liver disease (NAFLD) patients and animal models. The impact of QHD or its active components (geniposide and chlorogenic acid, GC) on NAFLD liver transcriptome and gut microbiota was examined with NAFLD rats. Increased expression for genes required for glutathione production and decreased expression for genes required for lipid synthesis was observed in NAFLD livers treated with QHD and GC. GC treatment decreased serum LPS, which could be explained by reduced mucosal damage in the colon of GC-treated rats. Further, our data suggest an increased abundance of Treg-inducing bacteria that stimulated the Treg activity in GC treated colon, which in turn down-regulated inflammatory signals, improved gut barrier function and consequently reduced hepatic exposure to microbial products. Our study suggests that QHD simultaneously enhanced the hepatic anti-oxidative mechanism, decreased hepatic lipid synthesis, and promoted the regulatory T cell inducing microbiota in the gut.

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“…Hepatic lipid contents in patients with choline deficiency have also been shown to affect gut microbial diversity [97]. Treatment with a combination of five Chinese herbs (Compositae : Polygonacease : Zingiberaceae : Clusiaceae : Rubiaceae = 13 : 7 : 7 : 7 : 7) was found to promote growth of short chain fatty acid producer Collinsella while improving steatosis in rats [98]. This altered gut microbiota associated with steatosis, particularly diminished Bacteroidetes abundance, may indicate gut dysbiosis and propagation of further hepatic injury.…”

Section: The Influence Of Gi Disease On Liver Injury and Regenerationmentioning

confidence: 99%

Implications of microbiota and bile acid in liver injury and regeneration

Liu

Keane

Sheng

et al. 2015

Journal of Hepatology

116

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Summary Studies examining the mechanisms by which the liver injures and regenerates usually focus on factors and pathways within the liver, neglecting the signaling derived from the gut-liver axis. The intestinal content is rich in microorganisms as well as metabolites generated from both the host and colonizing bacteria. Via the gut-liver axis, this complex “soup” exerts an immense impact on liver integrity and function. This review article summarizes data published in the past 30 years that have demonstrated the signaling derived from the gut-liver axis in relation to liver injury and regeneration. Despite many correlative findings, the intricate networks of pathways involved along with a scarcity of mechanistic data urgently require nutrigenomic, metabolomics, and microbiota profiling approaches to provide a deep understanding of the interplay between nutrition, bacteria, and host response. Such knowledge would better elucidate the molecular mechanisms that link microbiota alteration to host physiological response and vice-versa.

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Structural changes of gut microbiota in a rat non-alcoholic fatty liver disease model treated with a Chinese herbal formula

Cited by 72 publications

References 48 publications

Shifts in microbiota species and fermentation products in a dietary model enriched in fat and sucrose

Shifts in microbiota species and fermentation products in a dietary model enriched in fat and sucrose

Multi-targeting therapeutic mechanisms of the Chinese herbal medicine QHD in the treatment of non-alcoholic fatty liver disease

Implications of microbiota and bile acid in liver injury and regeneration

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