Structural Changes Manifested by Sertoli Cells During Experimental Allergic Orchitis in Guinea-Pigs

Kierszenbaum, Abraham L.; Mancini, R. E.

doi:10.1530/jrf.0.0330119

Cited by 18 publications

(4 citation statements)

References 7 publications

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“…Similar changes at this structure have been described in the testes of X-irradiated mice [16]. In the classic experimental orchitis induced by germinal cell antigens, the basement membrane of seminiferous tubules remain unchanged [9]. Therefore, the lesions described differ from the classic experimental allergic orchitis by its pathogenesis and histologic picture.…”

Section: Experimental Orchitis Induced In Rats 341supporting

confidence: 64%

Experimental Orchitis Induced in Rats by Passive Transfer of an Antiserum to Seminiferous Tubule Basement Membrane

Lustig

Denduchis

Gonzàlez

et al. 1978

Archives of Andrology

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A multifocal damage of the testis was obtained when rats were injected intravenously or under the tunica albuginea of the testis with a rabbit antiseminiferous tubule basement membrane serum. The damage was characterized by foci of perivascular and peritubular infiltrates of mononuclear round cells, infolding, thickening, and rupture of the seminiferous tubular wall and different degrees of injury of the germinal epithelium such as, cell disorganization, cell sloughing, and atrophy. Delamination and thickening of seminiferous tubule basement membrane and vacuolization of the Sertoli cell cytoplasm was often observed by electron microscopy. A linear deposit of rabbit gamma-globulin was detected by immunohistochemical techniques along the basement membranes of the seminiferous tubules and vessels. Testicular damage was not detected in rats injected with normal rabbit serum, used as control. In the kidneys of rats injected intravenously with the immune serum, a deposit of rabbit gamma-globulin was detected along glomerular basement membrane. Focal areas of mononuclear cell infiltrates, hypercellularity of glomeruli and thickening of glomerular capillary walls and Bowman's capsule were also observed.

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Section: Experimental Orchitis Induced In Rats 341supporting

confidence: 64%

Experimental Orchitis Induced in Rats by Passive Transfer of an Antiserum to Seminiferous Tubule Basement Membrane

Lustig

Denduchis

Gonzàlez

et al. 1978

Archives of Andrology

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“…Many tubules were virtually depleted of advanced germinal cells, leaving only Sertoli cells and basal compartment cells and a few, apically positioned germ cells comprising the epithelium. Sertoli cell vacuolation is also a commonly reported feature in conditions adverse to the testis (Kierszenbaum and Mancini, 1973;Jkgou et al, 1983;Young et al, 1988;Anderson et al, 1989;Kerr and Sharpe, 1989;Ghosh et al, 1990). Although never proven, we assume, as did Kierszenbaum and Mancini (19731, that intracellular vacuoles are produced when fluid buildup occurs in particular saccules of endoplasmic reticulum.…”

Section: Discussionmentioning

confidence: 63%

Testis structure in the sys (symplastic spermatids) mouse

Russell¹,

Hikim²,

Overbeek³

et al. 1991

Am. J. Anat.

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Testes of mice with the recessive insertional mutation termed symplastic spermatids (sys) were assessed for structural and developmental abnormalities. Homozygous (sys/sys) males are infertile due to an abnormality in spermatogenesis leading to azoospermia. The major interruption to spermatogenesis occurs when the intercellular bridges that connect round spermatids open prematurely resulting in the formation of symplasts. Symplasts contain as many as 285 nuclei. Development of spermatids within symplasts is arrested just before, or just after, elongation of the spermatid nuclei begins. Symplasts degenerate and appear to be phagocytized by Sertoli cells and by intratubular macrophages. In addition, degeneration of young round spermatids and also spermatocytes occasionally is observed. Spermatocyte degeneration is substantial in some tubules and leaves them depleted of cells other than basal compartment cells. Sertoli cell abnormalities are prominent and include intracellular vacuolation, absence of apical processes surrounding round spermatids, degeneration, and occasional sloughing. Although reduplication and infolding of the basal lamina is also seen, this does not appear as a common phenomenon. The sys phenotype is first manifest in animals between 19 days and 22 days of age. Considerable variability is seen in testis histology of prepubertal animals; some display degenerating pachytene spermatocytes and virtually no Sertoli cell vacuoles, while others display vacuoles without apparent elevated numbers of degenerating spermatocytes. Although this study has not revealed the primary cell type(s) affected by the insertional inactivation event, it is possible that the abnormalities in the Sertoli cells are responsible for germ cell degeneration as it is generally recognized that deficits in the Sertoli cell can result in major germ cell abnormalities but not vice versa.

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“…During an afternoon's literature search, one or two similar vesicles were noted in the section of the testis of a 14-year-old boy shown in Pugh's Pathology of the Testis (Brown, 1976). They were also noted in the illustrations of papers on mutant mice (Russell et al, 1991;Sawada et al, 1991), efferent duct ligation in the rat (Wang et al, 1985), seasonal changes in the American black bear (Tsubota et al, 1997), as well as experimental allergic orchitis in guinea pigs (Kierszenbaum and Mancini, 1973). Kierszenbaum and Mancini (1973) and Russell et al (1991) found them to arise from Sertoli cells, the former workers indicating that they arose by dilatation of smooth endoplasmic reticulum.…”

Section: Discussionmentioning

confidence: 87%

Effect of long-term vasectomy on seminiferous tubules in the guinea pig

et al. 1999

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The little previous work on the influence of vasectomy on the guinea pig testis has given controversial results. One group reports that the guinea pig suffers autoimmune orchitis while others claim damage may be mechanical. To clarify the issue, this study compares the morphology of seminiferous tubules 3 years after left unilateral vasectomy (8 guinea pigs) and control sham operation (6 animals). Grossly, left and right testes following left‐sided vasectomy were similar to controls and not significantly different in weight. On histology, left and right experimental testes and the control material showed various degrees of seminiferous tubular degeneration, including intraepithelial vesicle formation, loss of germ cells and intraluminal macrophages. Although vesicle formation was striking in most testes, quantitative analysis indicated that it was more frequent in the ipsilateral testis following unilateral vasectomy. It seems that vasectomy had exacerbated an age‐related phenomenon. Lymphocytic infiltration was seen in five of the left testes following vasectomy, in two of the corresponding right testes, but in none of the controls. Two vasectomized left testes, however, showed atrophic changes but no lymphocytic invasion. The results suggest that autoimmune orchitis follows vasectomy but that it may not be the primary cause of degeneration. Attempts to gain positive evidence for mechanical damage, however, were inconclusive. Clin. Anat. 12:250–263, 1999. © 1999 Wiley‐Liss, Inc.

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Structural Changes Manifested by Sertoli Cells During Experimental Allergic Orchitis in Guinea-Pigs

Cited by 18 publications

References 7 publications

Experimental Orchitis Induced in Rats by Passive Transfer of an Antiserum to Seminiferous Tubule Basement Membrane

Experimental Orchitis Induced in Rats by Passive Transfer of an Antiserum to Seminiferous Tubule Basement Membrane

Testis structure in the sys (symplastic spermatids) mouse

Effect of long-term vasectomy on seminiferous tubules in the guinea pig

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