2014
DOI: 10.1016/j.cell.2014.10.001
|View full text |Cite
|
Sign up to set email alerts
|

Structural Changes Enable Start Codon Recognition by the Eukaryotic Translation Initiation Complex

Abstract: SummaryDuring eukaryotic translation initiation, initiator tRNA does not insert fully into the P decoding site on the 40S ribosomal subunit. This conformation (POUT) is compatible with scanning mRNA for the AUG start codon. Base pairing with AUG is thought to promote isomerization to a more stable conformation (PIN) that arrests scanning and promotes dissociation of eIF1 from the 40S subunit. Here, we present a cryoEM reconstruction of a yeast preinitiation complex at 4.0 Å resolution with initiator tRNA in th… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
5

Citation Types

35
383
1

Year Published

2017
2017
2022
2022

Publication Types

Select...
5
1

Relationship

0
6

Authors

Journals

citations
Cited by 182 publications
(425 citation statements)
references
References 57 publications
35
383
1
Order By: Relevance
“…It releases phosphate as a consequence of the stable binding of the tRNAi anticodon to the start-codon. As observed for the scaffolding factors and 40S, eIF2 also makes minimal contact with the 40S, maintaining approximately 34 Å separation from the body of the subunit and passing above eIF1 and eIF1A [9,14,15,20]. The only significant contact is made with the head within the E-site adjacent to the P-site occupied by the tRNA itself [20].…”
Section: Start-codon Selection: To Stay or To Gomentioning
confidence: 91%
See 4 more Smart Citations
“…It releases phosphate as a consequence of the stable binding of the tRNAi anticodon to the start-codon. As observed for the scaffolding factors and 40S, eIF2 also makes minimal contact with the 40S, maintaining approximately 34 Å separation from the body of the subunit and passing above eIF1 and eIF1A [9,14,15,20]. The only significant contact is made with the head within the E-site adjacent to the P-site occupied by the tRNA itself [20].…”
Section: Start-codon Selection: To Stay or To Gomentioning
confidence: 91%
“…As observed for the scaffolding factors and 40S, eIF2 also makes minimal contact with the 40S, maintaining approximately 34 Å separation from the body of the subunit and passing above eIF1 and eIF1A [9,14,15,20]. The only significant contact is made with the head within the E-site adjacent to the P-site occupied by the tRNA itself [20]. This ensures that the ternary complex moves with the flexible 40S head in relation to the body of the subunit, while the contacts made with eIF1 and eIF1A will vary with head movement [9].…”
Section: Start-codon Selection: To Stay or To Gomentioning
confidence: 99%
See 3 more Smart Citations