Structural Changes at the Site of Tritrichomonas foetus-Erythrocyte Interaction.

Silva, Newton Scares da; Filho, Benedito Prado Dias; Souza, Wanderley de

doi:10.1247/csf.21.245

Cited by 4 publications

(7 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Da Silva et al [5] showed strong agglutination between T. foetus and human A group erythrocytes, suggesting the occurrence of an adhesin associated with the parasite surface. Indeed, the authors used the same K strain and human erythrocytes that we used in the present study and observed an increasing intensity of adhesion for up to 30 min of incubation.…”

Section: Discussionmentioning

confidence: 99%

“…In addition, CP inhibitors greatly reduce erythrocyte lysis, suggesting that CP may be a lytic factor involved in hemolysis [6]. Furthermore, studies focusing on the trichomonad-cell interaction have pointed out that the parasites can exert their cytopathic effects through: (a) physical contact established between the two cell surfaces, (b) toxins released from parasites into the interaction media, or (c) the association of both mechanisms [5].…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Tritrichomonas foetus: a scanning electron microscopy study of erythrocyte adhesion associated with hemolytic activity

2004

View full text Add to dashboard Cite

-The in vitro hemolytic activity of Tritrichomonas foetus was investigated. The parasite was tested against human erythrocytes of groups A, B, AB, and O, and against erythrocytes of nine adult animals of different species (the rabbit, rat, chicken, cat, dog, swine, horse, bovine, and sheep). The results showed that T. foetus strains (ATCC KV1, K, PAL, 5022, RJ, 90) did not present any hemolytic activity against any human erythrocyte group nor against rabbit, rat, chicken, cat, dog and swine erythrocytes. T. foetus strains, however, lysed horse, bovine, and sheep erythrocytes. No hemolysin released by the parasites could be identified. Hemolysis did not occur with trichomonad culture supernatants, with sonicated extracts of T. foetus, nor with killed organisms. Scanning electron microscopy (SEM) showed that human erythrocytes did not adhere to the trophozoites, in contrast horse erythrocytes adhered to the surface of the parasites and were phagocytosed for up to 90 min. The parasites are able to exert their cytopathic effects through: (a) physical contact established between the two cell surfaces, (b) toxins released from parasites into the interaction media, or (c) the association of both mechanisms. Further studies are necessary to clarify the importance of the hemolytic activity in the biology of T. foetus.Tritrichomonas foetus / hemolytic activity / scanning electron microscopy / erythrophagocytosis / cytopathic effects

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Tritrichomonas foetus: a scanning electron microscopy study of erythrocyte adhesion associated with hemolytic activity

2004

View full text Add to dashboard Cite

show abstract

“…Many molecules are thought to be involved in the adhesion processes of trichomonads. These include a glycoprotein present in T. foetus that recognizes sialic acid on the surface of target cells (Bonilha et al 1995;, adhesin-like proteins which promote hemaglutination in T. foetus (Da Silva et al 1996Silva et al , 1999, manose residues and other sugars and glycoconjugates located on the surface of T. foetus and/or Trichomonas vaginalis (Cappuccinelli et al 1975;Mirhaghani and Warton 1998;Benchimol and Bernardino 2002).…”

Section: Trophozoite Vs Pseudocyst Adhesion and Tf190 Expressionmentioning

confidence: 99%

Tritrichomonas foetus pseudocysts adhere to vaginal epithelial cells in a contact-dependent manner

Mariante

Lopes

Benchimol

2004

Parasitology Research

View full text Add to dashboard Cite

Tritrichomonas foetus is a parasitic protist of the urogenital tract of cattle. It presents the trophozoite stage, a motile elongated form that constitutes most of the cells in a normal population, and a pseudocyst stage, an immotile rounded form that appears under unfavourable environmental conditions. In the present report pseudocysts were studied in natural conditions and after induction by chemicals or cycles of cooling and warming of cultures. The capacity of T. foetus to adhere to vaginal epithelial cells (VECs) was compared for both trophozoite and pseudocyst forms. By the use of video-enhanced-contrast microscopy, scanning and transmission electron microscopy, and immunofluorescence microscopy techniques, we present evidence that: (1) T. foetus easily internalizes the flagella and forms pseudocysts under several unfavourable conditions; (2) T. foetus in both pseudocyst and trophozoite forms is able to adhere to VECs; (3) the adhesion rate is higher for pseudocysts than for trophozoites; (4) the adhesin Tf190 is expressed in both forms during interaction; (5) the adhesion process of pseudocysts seems to occur in a contact-dependent manner. Thus, we propose that the pseudocyst stage is not a degenerative form, but a functional life form that is able to interact with and firmly adhere to VECs.

show abstract

“…In the case of Tritrichomonas foetus, which is a protozoan found in the urogenital tract of cattle, there is very little information on basic aspects of the process of its interaction with cells from the host, although there are reports on the presence of receptors for extracellular-matrix proteins (Bonilha and Silva Filho 1990;). It has previously been shown that T. foetus, but not T. vaginalis, displays a high level of hemagglutinating activity and that during this process a focal adhesion, with changes in the structural organization of the plasma membrane, takes place (Silva et al 1996).This observation suggests that on its surface, T. foetus exposes molecules with an adhesin-like activity. In the present study we further analyzed this question and obtained evidence indicating the presence of a parasite surface protein with adhesin activity.…”

Section: Introductionmentioning

confidence: 95%

“…Tritrichomonas foetus (K strain) was maintained in TYM medium at 37°C (Diamond 1957) as previously described (Silva et al 1996).…”

Section: Microorganismmentioning

confidence: 99%

Identification and localization of an adhesin on the surface of Tritrichomonas foetus

Silva

Filho

Souza

1999

Parasitology Research

View full text Add to dashboard Cite

Tritrichomonas foetus is a mucosal parasite of the urogenital-vaginal tract of cattle that strongly adheres to erythrocytes, which suggests that it presents an adhesin that recognizes red blood cells from different animal species and blood groups. In the present report we describe a cell-fractionation method for obtainment of a membrane fraction of T. foetus, which adhered to red blood cells. The T. foetus adhesin was obtained after parasite lysis and fractionation followed by ultracentrifugation, whereby a 100,000-g pellet fraction showed a strong hemagglutinating activity. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis of this fraction, of erythrocyte ghosts, and of ghosts allowed to interact with the parasite membrane fraction revealed the presence of a 100-kDa protein as the putative adhesin. Polyclonal antibodies obtained in rabbits immunized with this protein recognized proteins of 100 and 90 kDa as determined by immunoblotting. Confocal laser scanning microscopy and transmission electron microscopy of cells incubated first in the presence of the antibody and subsequently in the presence of fluorescein- or gold-labeled goat anti-rabbit IgG showed labeling of the protozoan surface as well as of some cytoplasmic vesicles.

show abstract

Structural Changes at the Site of Tritrichomonas foetus-Erythrocyte Interaction.

Cited by 4 publications

References 37 publications

Tritrichomonas foetus: a scanning electron microscopy study of erythrocyte adhesion associated with hemolytic activity

Tritrichomonas foetus: a scanning electron microscopy study of erythrocyte adhesion associated with hemolytic activity

Tritrichomonas foetus pseudocysts adhere to vaginal epithelial cells in a contact-dependent manner

Identification and localization of an adhesin on the surface of Tritrichomonas foetus

Contact Info

Product

Resources

About