Structural Changes and Non-Photochemical Quenching of Chlorophyll a Fluorescence in Oxygenic Photosynthetic Organisms

Garab, Győző

doi:10.1007/978-94-017-9032-1_16

Cited by 5 publications

(5 citation statements)

References 141 publications

(212 reference statements)

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“…In excess light, sustained acidification of the lumen is sensed by PsbS protein in plants and triggers the qE through protonation of PSII proteins, it also activates the xanthophyll cycle [ 11 , 71 , 72 ]. In general, low pH and the light-induced transmembrane ΔpH have been shown to induce structural changes at different levels of structural complexity, at the microscopic levels affecting the distribution of protein complexes (for reviews see [ 22 , 73 ], the lipid phases [ 74 , 75 ], and assemblies at higher levels of the membrane organization [ 17 , 18 , 20 , 21 , 49 , 76 – 78 ]. While structural changes appear to be ubiquitous in oxygenic photosynthetic organisms, NPQ is not.…”

Section: Resultsmentioning

confidence: 99%

“…The functioning of the xanthophyll cycle (i.e. the de-epoxidation of violaxanthin to antheraxanthin and zeaxanthin) also involves significant reorganizations via the activity of the water-soluble, lipocalin-like enzyme violaxanthin de-epoxidase; the functioning of this enzyme requires the formation of a non-bilayer lipid phase [9,12,[16][17][18][19][20]23,27,[32][33][34]. In the sustained quenching component, qH, another lumenal lipocalin protein, LCNP, plays a central role [35].…”

Section: Introductionmentioning

confidence: 99%

“…Numerous studies have shown that fine adjustments of the photosynthetic functions to different environmental conditions depend largely on the structural flexibility of thylakoid membranes [1,[17][18][19][20][21][22][23][24]. Albeit details of the molecular mechanisms are still under debate, NPQ mechanisms have been thoroughly documented to be associated with structural changes in the thylakoid membranes.…”

Section: Introductionmentioning

confidence: 99%

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Thylakoid membrane reorganizations revealed by small-angle neutron scattering of Monstera deliciosa leaves associated with non-photochemical quenching

et al. 2020

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Non-photochemical quenching (NPQ) is an important photoprotective mechanism in plants and algae. Although the process is extensively studied, little is known about its relationship with ultrastructural changes of the thylakoid membranes. In order to better understand this relationship, we studied the effects of illumination on the organization of thylakoid membranes in Monstera deliciosa leaves. This evergreen species is known to exhibit very large NPQ and to possess giant grana with dozens of stacked thylakoids. It is thus ideally suited for small-angle neutron scattering measurements (SANS)—a non-invasive technique, which is capable of providing spatially and statistically averaged information on the periodicity of the thylakoid membranes and their rapid reorganizations in vivo . We show that NPQ-inducing illumination causes a strong decrease in the periodic order of granum thylakoid membranes. Development of NPQ and light-induced ultrastructural changes, as well as the relaxation processes, follow similar kinetic patterns. Surprisingly, whereas NPQ is suppressed by diuron, it impedes only the relaxation of the structural changes and not its formation, suggesting that structural changes do not cause but enable NPQ. We also demonstrate that the diminishment of SANS peak does not originate from light-induced redistribution and reorientation of chloroplasts inside the cells.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Thylakoid membrane reorganizations revealed by small-angle neutron scattering of Monstera deliciosa leaves associated with non-photochemical quenching

et al. 2020

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“…Several spectroscopy techniques, including transient absorption spectroscopy, fluorescence lifetime analysis, resonance Raman scattering, and circular dichroism, have observed and described in vitro significant reversible and quick reorganizations at the levels of (i) the ultrastructure of thylakoid membranes (Heber 1969; Krause 1973; Cseh et al 2005), (ii) macro-organization of Lhcs within the membrane (Dall’Osto et al 2005; Betterle et al 2009), and (iii) purified Lhcs (Moya et al 2001; Johnson and Ruban 2014). Although the relationship between NPQ and these different reorganizations remains to be further explored, provided evidence supports the idea that NPQ involves extensive reorganization of the Lhcs (Dall’Osto et al 2005; Betterle et al 2009; Garab 2016) opening energy dissipation channels that would trap the energy transferred amongst Chls (Krüger and van Grondelle 2017). However, as in vitro analysis of possible molecular mechanisms of NPQ are never performed on antenna complexes in their native environment (Krüger and van Grondelle 2017), the molecular basis and strengths of different energy-quenching mechanisms in vivo remains still unexplored (Holzwarth et al 2009; Nilkens et al 2010).…”

Section: Introductionmentioning

confidence: 84%

In vivo photoprotection mechanisms observed from leaf spectral absorbance changes showing VIS–NIR slow-induced conformational pigment bed changes

et al. 2019

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Regulated heat dissipation under excessive light comprises a complexity of mechanisms, whereby the supramolecular light-harvesting pigment–protein complex (LHC) shifts state from light harvesting towards heat dissipation, quenching the excess of photo-induced excitation energy in a non-photochemical way. Based on whole-leaf spectroscopy measuring upward and downward spectral radiance fluxes, we studied spectrally contiguous (hyperspectral) transient time series of absorbance A(λ,t) and passively induced chlorophyll fluorescence F(λ,t) dynamics of intact leaves in the visible and near-infrared wavelengths (VIS–NIR, 400–800 nm) after sudden strong natural-like illumination exposure. Besides light avoidance mechanism, we observed on absorbance signatures, calculated from simultaneous reflectance R(λ,t) and transmittance T(λ,t) measurements as A(λ,t) = 1 − R(λ,t) − T(λ,t), major dynamic events with specific onsets and kinetical behaviour. A consistent well-known fast carotenoid absorbance feature (500–570 nm) appears within the first seconds to minutes, seen from both the reflected (backscattered) and transmitted (forward scattered) radiance differences. Simultaneous fast Chl features are observed, either as an increased or decreased scattering behaviour during quick light adjustment consistent with re-organizations of the membrane. The carotenoid absorbance feature shows up simultaneously with a major F decrease and corresponds to the xanthophyll conversion, as quick response to the proton gradient build-up. After xanthophyll conversion (t = 3 min), a kinetically slower but major and smooth absorbance increase was occasionally observed from the transmitted radiance measurements as wide peaks in the green (~ 550 nm) and the near-infrared (~ 750 nm) wavelengths, involving no further F quenching. Surprisingly, in relation to the response to high light, this broad and consistent VIS–NIR feature indicates a slowly induced absorbance increase with a sigmoid kinetical behaviour. In analogy to sub-leaf-level observations, we suggest that this mechanism can be explained by a structure-induced low-energy-shifted energy redistribution involving both Car and Chl. These findings might pave the way towards a further non-invasive spectral investigation of antenna conformations and their relations with energy quenching at the intact leaf level, which is, in combination with F measurements, of a high importance for assessing plant photosynthesis in vivo and in addition from remote observations.Electronic supplementary materialThe online version of this article (10.1007/s11120-019-00664-3) contains supplementary material, which is available to authorized users.

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“…We argued and edited several chapters with great vigor. For his many novel ideas on the relation of NPQ with the structural and molecular changes in chloroplasts, see his in-depth review (Garab 2014) in this book.…”

Section: The 2000s -At Conferences and Collaboration In Editing The N...mentioning

confidence: 99%

In appreciation of an ingenious scientist and a great friend: Győző Garab

GOVINDJEE

2023

Photosynt.

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Structural Changes and Non-Photochemical Quenching of Chlorophyll a Fluorescence in Oxygenic Photosynthetic Organisms

Cited by 5 publications

References 141 publications

Thylakoid membrane reorganizations revealed by small-angle neutron scattering of Monstera deliciosa leaves associated with non-photochemical quenching

Thylakoid membrane reorganizations revealed by small-angle neutron scattering of Monstera deliciosa leaves associated with non-photochemical quenching

In vivo photoprotection mechanisms observed from leaf spectral absorbance changes showing VIS–NIR slow-induced conformational pigment bed changes

In appreciation of an ingenious scientist and a great friend: Győző Garab

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