Structural changes and enzymatic response of Napier grass (Pennisetum purpureum) stem induced by alkaline pretreatment

Phitsuwan, Paripok; Sakka, Kazuo; Ratanakhanokchai, Khanok

doi:10.1016/j.biortech.2016.06.089

Cited by 124 publications

(67 citation statements)

References 34 publications

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“…Thus, during enzymatic hydrolysis, the enzymes tend to bind irreversibly to the lignin by means of hydrophobic interactions, which causes loss of activity and the requirement of a greater enzymatic charge in the process [68]. Lignin acts negatively on enzymatic hydrolysis by three main factors: competitive adsorption, chemical inhibition and steric hindrance [44,69].…”

Section: Resultsmentioning

confidence: 99%

Composition and growth of sorghum biomass genotypes for ethanol production

Almeida

Parrella

Simeone

et al. 2019

Biomass and Bioenergy

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A B S T R A C TSorghum bicolor (L.) (Moench), which stands out for dry matter yield per hectare, has been considered as potential raw material for biofuels and electricity generation. It has a production cycle of six months, possibility of mechanization of cultivation and harvest and good adaptation to most regions of Brazil. Sorghum genotypes were evaluated for agronomic potential and chemical composition favorable to the production of second-generation ethanol. Three brown midrib (bmr) sorghum mutant hybrids were compared to three conventional hybrids. The bmr sorghum mutant hybrids are associated with reduced lignin content, making these genotypes more promising to the enzymatic conversion processes of the biomass. Sorghum biomass showed a high potential in terms of biomass production, with an average dry matter yield of 26.57 Mg ha −1 . Brown midrib sorghum hybrids showed significantly lower lignin contents than conventional hybrids and demonstrated the potential for cellulosic ethanol production.

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Section: Resultsmentioning

confidence: 99%

Composition and growth of sorghum biomass genotypes for ethanol production

Almeida

Parrella

Simeone

et al. 2019

Biomass and Bioenergy

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“…The breakdown of cellulose into glucose is fundamental for 2G ethanol production, irrespective of the cellulose content of biomass samples. Elephant grass has high cellulose content, which makes this biomass source particularly attractive from the perspective of fermentable sugar production, and consequently ethanol production (Phitsuwan et al, 2016). In addition, the lower lignin content found in elephant grass compared with other lignocellulosic materials likely results in a lower inherent recalcitrance, and hence facilitates cell wall disruption and the efficient release of fermentable monomeric sugars (Brienzo et al, 2017;Wallace et al, 2016).…”

Section: Chemical Composition Of Elephant Grass Fractionmentioning

confidence: 99%

Elephant grass leaves have lower recalcitrance to acid pretreatment than stems, with higher potential for ethanol production

Santos

Souza

Anna

et al. 2018

Industrial Crops and Products

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Elephant grass is gaining attention among lignocellulosic materials due to its high growth potential, biomass yield, limited requirement for cultivation land and high rates of carbon dioxide absorption. Here was investigate the effect of pretreatment with different concentrations (5, 10 and 20%, mass acid/mass material) of diluted sulfuric acid on the whole elephant grass plant compared with its leaf and stem fractions. The stem was the most recalcitrant fraction, judging from the high recovery of water insoluble solids (WIS) and lower enzymatic hydrolysis yield, upon acid pretreatment. In enzymatic hydrolysis assays, the glucose yield increased with increasing concentrations of acid, reaching maximum values of 89.20 (leaf), 43.54 (stem) and 76.01% (whole plant). The crystallinity index (CrI) increased in both elephant grass fractions, which correlated with the solubilization of amorphous materials such as hemicellulose. Also, the stem fraction had a slightly higher heating value than the leaf fraction (3958.45 and 3939.49 cal/g, respectively). Scanning electron microscopy (SEM) analysis showed drastic morphological changes in the samples with increasing pretreatment severity, although the stem fraction suffered less structural damage than other materials. Taken together, the results suggest that the separation of elephant grass in different fractions decreases biomass heterogeneity and generates a fraction (leaf) with lower inherent recalcitrance and, thus, higher susceptibility to pretreatment and enzymatic hydrolysis, increasing the efficiency of fermentable sugar release. The results indicate that the leaf fraction of elephant grass has higher potential for use in second-generation ethanol production, while the stem fraction may be more useful for energy co-generation by combustion.

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“…Aqueous ammonia pretreatment is a widely studied method to alter the structure and reduce the recalcitrance of biomass for enzymatic hydrolysis [8][9][10][11][12][13]. This method has potential to be applied in the biorefinery industry because it separates biomass components into two main fractions: carbohydrates and lignin stream owing to the delignification selectivity.…”

Section: Introductionmentioning

confidence: 99%

“…This method has potential to be applied in the biorefinery industry because it separates biomass components into two main fractions: carbohydrates and lignin stream owing to the delignification selectivity. Different types of lignocellulosic materials, including Napier grass [8,10], rye straw [9], poplar [11], corn stover [12], and switchgrass [13], have been tested with aqueous ammonia pretreatment, and those reports have addressed positive correlation of chemical composition change (particularly high rate of lignin removal) and enzymatic digestibility.…”

Section: Introductionmentioning

confidence: 99%

Structural Analysis of Alkaline Pretreated Rice Straw for Ethanol Production

Phitsuwan

Permsriburasuk

Baramee

et al. 2017

International Journal of Polymer Science

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Rice straw (RS) is an abundant, readily available agricultural waste, which shows promise as a potential feedstock for Asian ethanol production. To enhance release of glucose by enzymatic hydrolysis, RS was pretreated with aqueous ammonia (27% w/w) at two pretreatment temperatures: room temperature and 60 ∘ C. Statistical analysis indicated similarity of enzymatic glucose production at both pretreatment temperatures after 3-day incubation. Chemical composition, FTIR, and EDX analyses confirmed the retention of glucan and xylan in the pretreated solid, but significant reduction of lignin (60.7% removal) and silica. SEM analysis showed the disorganized surfaces and porosity of the pretreated RS fibers, thus improving cellulose accessibility for cellulase. The crystallinity index increased from 40.5 to 52.3%, indicating the higher exposure of cellulose. With 10% (w/v) solid loadings of pretreated RS, simultaneous saccharification and fermentation yielded a final ethanol concentration of 24.6 g/L, corresponding to 98% of maximum theoretical yield. Taken together, aqueous ammonia pretreatment is an effective method to generate highly digestible pretreated RS for bioethanol production and demonstrates potential application in biorefinery industry.

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Structural changes and enzymatic response of Napier grass (Pennisetum purpureum) stem induced by alkaline pretreatment

Cited by 124 publications

References 34 publications

Composition and growth of sorghum biomass genotypes for ethanol production

Composition and growth of sorghum biomass genotypes for ethanol production

Elephant grass leaves have lower recalcitrance to acid pretreatment than stems, with higher potential for ethanol production

Structural Analysis of Alkaline Pretreated Rice Straw for Ethanol Production

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