Structural Biology of the Fibres of the Collagenous and Elastic Systems

Montes, G. S.

doi:10.1006/cbir.1996.0004

Cited by 342 publications

(336 citation statements)

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“…served in human skin (Cotta-Pereira et al, 1976, 1977Hollister et al, 1990;Montes, 1996), and even in the skin of a teleost (Ferri, 1980). In human skin, oxytalan microfibrils emerge from the dermoepidermal junction, run through the entire papillary dermis as elaunin fibers with an increasing but limited amount of elastin, and finally merge with horizontally arranged, thick elastic fibers invested with elastin.…”

Section: Discussionmentioning

confidence: 99%

Subectodermal microfibrillar bundles are organized into a distinct parallel array in the developing chick limb bud

et al. 2004

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In this study, a unique fiber system in the subectodermal mesenchyme of the chick limb bud was visualized immunohistochemically with the use of a novel monoclonal antibody termed "FB1." This antibody stained a subset of extracellular fibers in the embryonic mesenchyme. Among the fibers visualized, those running perpendicularly to the limb bud ectoderm became progressively prominent in their thickness and length, and organized into a parallel array in the subectodermal region. This fiber system was distinct from that of major collagens, fibronectin, or tenascin. A molecule immunoprecipitated with FB1 comigrated with JB3 antigen, or chicken fibrillin-2. The fibers visualized immunohistochemically by FB1 and JB3 were indistinguishable from each other, and ultrastructurally appeared to be bundles composed of tubular-like microfibrils that originated directly from the ectodermal basal lamina. They lacked the amorphous deposits that are characteristic of elastin. A similar array of subectodermal fibers was also found in the developing axilla and some truncal regions, again well before the development of a definitive dermis. These findings suggest that a parallel array of subectodermal FB1-positive fibers constitutes a precocious fiber system in the presumptive dermis prior to the substantial formation of collagenous fibers. These fibers could be developmentally linked to oxytalan fibers, which are known to be present in the papillary dermis in mature cutaneous tissue.

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Section: Discussionmentioning

confidence: 99%

Subectodermal microfibrillar bundles are organized into a distinct parallel array in the developing chick limb bud

et al. 2004

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“…Detailed methods used to obtain ii, iii and iv have been published previously [61,62] component relative to the microfibrillar component; and oxytalan fibres, which have no elastin component and are thus composed entirely of microfibrils [44]. As well as existing as fully developed fibres in their own right, during development, elaunin and oxytalan fibres may appear as progenitors of mature elastic fibres [44]. Henceforth, the use of the term 'elastic fibres' in this review will encompass all three fibre types.…”

Section: Elastic Fibresmentioning

confidence: 99%

The elastic fibre network of the human lumbar anulus fibrosus: architecture, mechanical function and potential role in the progression of intervertebral disc degeneration

Smith

Fazzalari

2009

Eur Spine J

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Elastic fibres are critical constituents of dynamic biological structures that functionally require elasticity and resilience. The network of elastic fibres in the anulus fibrosus of the intervertebral disc is extensive, however until recently, the majority of histological, biochemical and biomechanical studies have focussed on the roles of other extracellular matrix constituents such as collagens and proteoglycans. The resulting lack of detailed descriptions of elastic fibre network architecture and mechanical function has limited understanding of the potentially important contribution made by elastic fibres to healthy disc function and their possible roles in the progression of disc degeneration. In addition, it has made it difficult to postulate what the consequences of elastic fibre related disorders would be for intervertebral disc behaviour, and to develop treatments accordingly. In this paper, we review recent and historical studies which have examined both the structure and the function of the human lumbar anulus fibrosus elastic fibre network, provide a synergistic discussion in an attempt to clarify its potentially critical contribution both to normal intervertebral disc behaviour and the processes relating to its degeneration, and recommend critical areas for future research.

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“…Os cortes incluí-dos em parafina foram corados pela hematoxilina-eosina para análise histopatológica e os cortes incluídos em glicol metacrilato foram corados pelo azul de toluidina para análise histomorfométrica da cápsula, dos seios (subcapsular e medular), dos folículos linfóides e dos cordões medulares. Para cada amostra, a avaliação das fibras colágenas da matriz extracelular foi realizada através do método picrosirius polarização (Montes 1996). Para isso, os cortes foram desparafinados, hidratados e corados por uma hora em solução de Sirius Red 0,1%, dissolvida em ácido pícrico aquoso saturado e posteriormente lavados em água corrente e contracorados com hematoxilina de Harris durante três minutos.…”

Section: Methodsunclassified

Análise histomorfométrica da matriz extracelular do linfonodo poplíteo de cães naturalmente infectados por Leishmania (L.) chagasi

Kondo

Fonseca

Matta³

et al. 2009

Pesq. Vet. Bras.

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Nas Américas, a leishmaniose visceral canina é causada por Leishmania (Leishmania) chagasi, um protozoário intracelular obrigatório do sistema fagocítico mononuclear; as principais alterações histológicas associadas a essa doença ocorrem nos em órgãos linfóides. Apesar de o cão ser considerado o principal mantenedor e disseminador da leishmaniose no ambiente urbano, são escassos estudos dos aspectos histopatológicos e histomorfométricos, em cães naturalmente infectados com L. chagasi, que investiguem a interação entre o parasito e a matriz extracelular. Este estudo visou caracterizar e quantificar as alterações dos componentes celulares e da matriz extracelular (colágenos I e III) do linfonodo poplíteo de 22 cães com infecção natural por L. chagasi detectada através da reação de imunofluorescência indireta (RIF) e compará-las com as alterações encontradas no linfonodo poplíteo de 10 cães não-infectados, negativos na RIF e clinicamente saudáveis. Fragmentos dos linfonodos foram seccionados longitudinalmente, processados rotineiramente para exame histológico e corados por hematoxilina-eosina. Cortes adicionais do mesmo linfonodo incluídos em glicol metacrilato foram corados pelo azul de toluidina para histomorfometria. Linfonodos de cães infectados apresentaram linfadenopatia generalizada, aumento do tamanho e do número dos folículos linfóides, hipertrofia da cápsula e hiperplasia linfóide significativa. Nos linfonodos de cães do grupo infectado, a análise quantitativa de fibras colágenas mostrou significativo predomínio do colágeno I sobre o colágeno III. Esses resultados demonstram que cães infectados por L. chagasi apresentam degradação dos constituintes da matriz extracelular e conseqüente destruição do arcabouço linfóide, alterando a morfologia do órgão.
In the Americas, canine visceral leishmaniasis is caused by Leishmania (Leishmania) chagasi, an obligatory intracellular parasite of the phagocytic-monocytic system; the main histological changes associated with this disease occur in the lymphoid organs. Although dogs are considered to be the main carriers and disseminators of leishmaniasis in urban areas, there are few studies on the histopathologic and histomorphometric aspects in dogs naturally infected by L.chagasi analyzing the interaction between parasite and extracellular matrix. The current study characterize and quantify changes in the cellular and extracellular matrix (collagens type I and III) components of the popliteal lymph node from of 22 dogs with the natural infection by L. chagasi confirmed by indirect immuno-fluorescence assay (IFA) and compare theses findings with those fund in the popliteal lymph node from 10 non-infected dogs, that reacted negative in the IFA, and were clinically healthy. Lymph node fragments were longitudinally sliced and sections were processed for routine histopathology and stained by hematoxylin and eosin. For histomorphometry, additional sections from the same lymph node were fixed in glycol methacrylate and stained with toluidine blue. Lymph nodes from affected d...

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Structural Biology of the Fibres of the Collagenous and Elastic Systems

Cited by 342 publications

References 0 publications

Subectodermal microfibrillar bundles are organized into a distinct parallel array in the developing chick limb bud

Subectodermal microfibrillar bundles are organized into a distinct parallel array in the developing chick limb bud

The elastic fibre network of the human lumbar anulus fibrosus: architecture, mechanical function and potential role in the progression of intervertebral disc degeneration

Análise histomorfométrica da matriz extracelular do linfonodo poplíteo de cães naturalmente infectados por Leishmania (L.) chagasi

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