Structural Basis of the Honey Bee PBP Pheromone and pH-induced Conformational Change

BmorPBP1, BmorPBP2 and BmorABPx also bind the pheromone component (10E,12Z)-hexadecadienal (bombykal) equally well, whereas BmorGOBP2 can discriminate between bombykol and bombykal. X-ray structures show that when bombykol is bound to BmorGOBP2 it adopts a different conformation from that found when it binds to BmorPBP1. Binding to BmorGOBP2 involves hydrogen bonding to Arg110 rather than to the Ser56 as found for BmorPBP1.

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“…63 In BmorPBP1/bombykol structure the Cterminus blocks the rear entry to the pocket with Val135 coming within 4Å of bombykol.…”

Section: Discussionmentioning

confidence: 99%

Characterisation of Bombyx mori Odorant-binding Proteins Reveals that a General Odorant-binding Protein Discriminates Between Sex Pheromone Components

Zhou

Robertson

et al. 2009

Journal of Molecular Biology

233

249

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“…A more meaningful phylogenetic tree of insect GOBPs can be constructed when more GOBPs become available from a larger number of species. Insect OBPs have also been classified into long chain (∼160 aa), medium chain (∼120 aa), and short chain (∼110 aa) classes, which relates to their potential structure and function (Pesenti et al 2008). According to this hypothesis, PxylGOBP1 (168 aa) and PxylGOBP2 (163 aa) belong to the long chain class, and this is useful to know for future functional studies of these proteins.…”

Section: Discussionmentioning

confidence: 99%

Molecular Characterization and Expression Pattern of Two General Odorant Binding Proteins from the Diamondback Moth, Plutella xylostella

Zhang

Wang

et al. 2009

J Chem Ecol

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In the Lepidoptera, odorant signals are thought to be mediated by general odorant binding proteins (GOBPs) in the sensillar lymph surrounding the olfactory receptors. We describe the identification and characterization of two new cDNAs encoding GOBPs from the antennae of the diamondback moth, Plutella xylostella (L.) (Lepidoptera: Plutellidae), a species for which no GOBPs have been identified to date. We focused our investigation on this olfactory protein family by using reverse transcription-polymerase chain reaction strategies. The deduced amino acid sequences of PxylGOBP1 and PxylGOBP2 revealed open reading frames of 168 and 163 amino acids, respectively, with six cysteine residues in conserved positions relative to other known GOBPs. The alignment of the mature PxylGOBPs with other Lepidoptera GOBPs showed high sequence identity (70-80%) with other full-length sequences from GenBank. Sequence identity between PxylGOBP1and PxylGOBP2 was only 50%, suggesting that the two proteins belong to different classes of lepidopteran GOBPs. The expression patterns of the two PxylGOBP genes, with respect to tissue distribution and sex, were further investigated by reverse transcription-polymerase chain reaction (RT-PCR) and real-time PCR. Although the two GOBP genes were expressed only in the antennae of both sexes, reflecting the antennal specificity of GOBPs, the transcription levels of these genes depended on the sex, the age, the mating status, and the genes.

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“…The volume of this pocket is bigger than the internal cavity of the Antheraea polyphemus PBP ApolPBP without ligand which has a volume of 282 Å 3 26 and that of Bombyx mori PBP BmorPBP1 which has a volume of 167 Å 3 27 . However, it is smaller than the 492 Å 3 of A. mellifera OBP AmelASP1 28 and the 435 Å 3 of Leucophaea maderae OBP LmadPBP…”

Section: Resultsmentioning

confidence: 78%

Crystal structures and binding dynamics of odorant-binding protein 3 from two aphid speciesMegoura viciaeandNasonovia ribisnigri

Northey¹

2016

2016 International Congress of Entomology

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Structural Basis of the Honey Bee PBP Pheromone and pH-induced Conformational Change

Cited by 75 publications

References 38 publications

Characterisation of Bombyx mori Odorant-binding Proteins Reveals that a General Odorant-binding Protein Discriminates Between Sex Pheromone Components

Characterisation of Bombyx mori Odorant-binding Proteins Reveals that a General Odorant-binding Protein Discriminates Between Sex Pheromone Components

Molecular Characterization and Expression Pattern of Two General Odorant Binding Proteins from the Diamondback Moth, Plutella xylostella

Crystal structures and binding dynamics of odorant-binding protein 3 from two aphid speciesMegoura viciaeandNasonovia ribisnigri

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