2009
DOI: 10.1016/j.str.2008.12.008
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Structural Basis of Murein Peptide Specificity of a γ-D-Glutamyl-L-Diamino Acid Endopeptidase

Abstract: Crystal structures of two homologous peptidases from cyanobacteria Anabaena variabilis and Nostoc punctiforme at 1.05 Å and 1.60 Å resolution represent the first structures of a large class of cell-wall, cysteine peptidases that contain an N-terminal bacterial SH3-like domain (SH3b) and a C-terminal NlpC/P60 cysteine peptidase domain. The NlpC/P60 domain is a primitive, papain-like peptidase in the CA clan of cysteine peptidases with a Cys126/His176/His188 catalytic triad and a conserved catalytic core. We ded… Show more

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Cited by 67 publications
(101 citation statements)
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“…In this superfamily of papain-like enzymes, a conserved cysteine residue was predicted to be a catalytic residue on amino acid sequence alignment (2,16). Recently, the three-dimensional structures of NlpC/P60 enzymes were reported (Spr from E. coli, ABA23003 from Anabaena variabilis, and ACC79413 from Nostoc punctiforme) (3,26). In these enzymes, the conserved cysteine residues are located at a predicted active site and are structurally conserved.…”
Section: Dl-endopeptidase Activity Of Lyte or Cwlo Is Essential For mentioning
confidence: 99%
“…In this superfamily of papain-like enzymes, a conserved cysteine residue was predicted to be a catalytic residue on amino acid sequence alignment (2,16). Recently, the three-dimensional structures of NlpC/P60 enzymes were reported (Spr from E. coli, ABA23003 from Anabaena variabilis, and ACC79413 from Nostoc punctiforme) (3,26). In these enzymes, the conserved cysteine residues are located at a predicted active site and are structurally conserved.…”
Section: Dl-endopeptidase Activity Of Lyte or Cwlo Is Essential For mentioning
confidence: 99%
“…Structural analyses of Spr (39), AvPCP and NpPCP endopeptidases from cyanobacteria (48), and YkfC from B. cereus (BcYkfC) (49), from the NlpC/P60 family, showed a characteristic Cys-His-His catalytic triad in the active sites of these cys- teine peptidases. In the deduced active sites of LytF, LytE, CwlS, CwlO, and PgdS (shown as red arrowheads in Fig.…”
Section: Structural and Surface Properties Of Catalytic Domains Of DLmentioning
confidence: 99%
“…PcsB contains a CHAP domain found in some phage and bacterial murein amidases and endopeptidases ( Fig. 1) (2,29,56,71). PcsB is a relatively abundant (ϳ5,000 monomers per cell) protein whose function is essential, although bypass suppressors can be isolated (1; unpublished data).…”
mentioning
confidence: 99%