Structural basis for the recognition of the 2019-nCoV by human ACE2

Yan, Renhong; Zhang, Yuanyuan; Guo, Yingying; Xia, Lu; Zhou, Qiang

doi:10.1101/2020.02.19.956946

Cited by 133 publications

(95 citation statements)

References 7 publications

(9 reference statements)

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“…The structure of protein complex between RBD region of S protein of SARS-CoV and human ACE2 has been resolved (PDB: 2AJF) [7]. Recently, the structure of SARS-CoV-2 RBD with human ACE2 was also determined [16,17]. We used Chimera software to display homologous model, and obtained the interaction complex structure of RBD region of SARSr-CoV (SARS-CoV-2 and SARS-CoV) and cat/dog/pangolin/Chinese hamster ACE2.…”

Section: Structure Simulation Of the Protein Complex Of Sarsr-cov Rbdmentioning

confidence: 99%

Spike protein recognition of mammalian ACE2 predicts the host range and an optimized ACE2 for SARS-CoV-2 infection

Luan

Jin

et al. 2020

Biochemical and Biophysical Research Communications

360

403

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a b s t r a c t SARS-CoV-2 causes the recent global COVID-19 public health emergency. ACE2 is the receptor for both SARS-CoV-2 and SARS-CoV. To predict the potential host range of SARS-CoV-2, we analyzed the key residues of ACE2 for recognizing S protein. We found that most of the selected mammals including pets (dog and cat), pangolin and Circetidae mammals remained the most of key residues for association with S protein from SARS-CoV and SARS-CoV-2. The interaction interface between cat/dog/pangolin/Chinese hamster ACE2 and SARS-CoV/SARS-CoV-2 S protein was simulated through homology modeling. We identified that N82 in ACE2 showed a closer contact with SARS-CoV-2 S protein than M82 in human ACE2. Our finding will provide important insights into the host range of SARS-CoV-2 and a new strategy to design an optimized ACE2 for SARS-CoV-2 infection.Please cite this article as: J. Luan et al., Spike protein recognition of mammalian ACE2 predicts the host range and an optimized ACE2 for SARS-CoV-2 infection, Biochemical and Biophysical Research Communications, https://doi.

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Section: Structure Simulation Of the Protein Complex Of Sarsr-cov Rbdmentioning

confidence: 99%

Spike protein recognition of mammalian ACE2 predicts the host range and an optimized ACE2 for SARS-CoV-2 infection

Luan

Jin

et al. 2020

Biochemical and Biophysical Research Communications

360

403

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“…Research has confirmed that SARS-CoV-2 infects host cells by binding to angiotensin converting enzyme II (ACE2), [10][11][12] and ACE2 is highly expressed in nasopharyngeal cells. 13 More importantly, current research detected a high viral load in the nasopharynx soon after symptom onset.…”

Section: (Which Was Not Peer-reviewed)mentioning

confidence: 99%

Caution: The clinical characteristics of COVID-19 patients at admission are changing

Chen

Zhang

et al. 2020

Preprint

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Background.With the emergence of 4 rd generation transmission, the prevention and treatment of the novel coronavirus disease 2019 has entered a new period.We aimed to report several changes in the clinical characteristics at admission of patients with COVID-19. Methods.Clinical records and laboratory results of patients suffering from COVID-19were retrospectively reviewed and matched with the admission dates to analyze the changes in characteristics at the onset of illness. Results. Of the 89 affected patients, 31 [34.8%] patients were admitted from January 16 to 22, and 58 [65.2%] were admitted from January 23 to 29. Patients were admitted with more systemic symptoms, such as fever (21 [67.7%] of 31), fatigue (13 [41.9%] of 31), and myalgia (7 [22.6%] of 31), before January 23. More patients (10 [32.3%] of 31) admitted before January 23 had a small amount of sputum production compared with a smaller proportion (4 [6.9%] of 58) of the patients admitted after January 23.Other symptoms, such as cough, nausea, diarrhea, and chest tightness, were not significantly different between the two groups. In addition, the group admitted before January 23 had a larger proportion of patients with reduced lymphocyte (13 [54.2%] of 24), CD3 (11 [54.4%] of 21), and CD8 (9 [42.9%] of 21) counts and elevated serum amyloid A (SAA, 18 [75%] of 24).Conclusions. The initial symptoms of recently infected patients seem more insidious, indicating that the new coronavirus may gradually evolve into a virus similar to influenza and latent in asymptomatic carriers for a long time.

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“…It has been proved that SARS-CoV-2 uses ACE2 as a viral receptor for entry process [2] . ACE2 mRNA is highly expressed and stabilized by B 0 AT1 in gastrointestinal system [3,4], providing a prerequisite for SARS-CoV-2 infection. To further investigate the clinical significance of SARS-CoV-2 RNA in feces, we examined the viral RNA in feces from 71 patients with SARS-CoV-2 infection during their hospitalization.…”

mentioning

confidence: 99%