2007
DOI: 10.1038/nsmb1327
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Structural basis for the coevolution of a viral RNA–protein complex

Abstract: The cocrystal structure of the PP7 bacteriophage coat protein in complex with its translational operator identifies a distinct mode of sequence-specific RNA recognition when compared to the well-characterized MS2 coat protein-RNA complex. The structure reveals the molecular basis of the PP7 coat protein's ability to selectively bind its cognate RNA, and it demonstrates that the conserved β-sheet surface is a flexible architecture that can evolve to recognize diverse RNA hairpins.The inherent sequence diversity… Show more

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Cited by 228 publications
(223 citation statements)
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“…Others have used it to study the association of bacteriophage PP7 coat protein and vertebrate ZBP variants to their cognate RNA sequences (Chao et al 2008(Chao et al , 2010. These results show that the method is applicable to a wide variety of protein-RNA complexes.…”
Section: Introductionmentioning
confidence: 85%
“…Others have used it to study the association of bacteriophage PP7 coat protein and vertebrate ZBP variants to their cognate RNA sequences (Chao et al 2008(Chao et al , 2010. These results show that the method is applicable to a wide variety of protein-RNA complexes.…”
Section: Introductionmentioning
confidence: 85%
“…A vector transformation map (affine transformation matrix) was used to then correct the images for chromatic aberration. with high affinity (14,21,22). To confirm the intracellular association between MCP-GFP and single-β-actin mRNA molecules within the cell, we performed single-molecule FISH in combination with immunofluorescence (smFISH-IF) in neurons ( Fig.…”
Section: Resultsmentioning
confidence: 99%
“…The distance for MCP-GFP to β-actin mRNA is estimated at 7 nm. The drawing of the molecules was generated in PyMol software with the help of published structure data (22,44).…”
Section: Resultsmentioning
confidence: 99%
“…Since the seminal work of Altschuh identifying correlated amino acid changes in Tobacco mosaic virus (Altschuh et al 1987), studies recognizing co-evolution as an indicative, albeit subtle, signal of PPIs have being reported in the literature (Travers and Fares 2007;Chao et al 2008;Presser et al 2008). Co-evolutionary information may be divided into three groups: the simultaneous loss or gain of orthologous genes , correlated changes affecting both interacting partners at whole sequence level (explored by mirrortree-based approaches) (Goh and Cohen 2002;Hakes et al 2007;Juan et al 2008) or single amino acids changes (Mintseris and Weng 2005;Madaoui and Guerois 2008).…”
Section: Co-evolution Methodsmentioning
confidence: 99%