Structural Basis for Intermodular Communication in Assembly-Line Polyketide Biosynthesis
Dillon P. Cogan,
Alexander M. Soohoo,
Muyuan Chen
et al.
Abstract:Assembly-line polyketide synthases are large multienzyme systems with considerable potential for genetic reprogramming. To investigate the mechanisms by which reactive biosynthetic intermediates are directionally channeled across a defined sequence of active sites in a naturally occurring assembly line, we employed a bifunctional reagent to crosslink transient domain-domain interfaces of the 6-deoxyerythronolide B synthase. Structural resolution of these crosslinked states by single-particle cryogenic electron… Show more
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