Structural basis for blue-green light harvesting and energy dissipation in diatoms

Wang, Wenda; Yu, Long; Xu, Caizhe; Tanaka, Takashi; Zhao, Songhao; Umena, Yasufumi; Chen, Xiaobo; Qin, Xiaochun; Xin, Yueyong; Suga, Michihiro; Han, Guangye; Kuang, Tingyun; Shen, Jian Ren

doi:10.1126/science.aav0365

Cited by 196 publications

(274 citation statements)

References 63 publications

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“…However, this motif is absent in FCPs, and characteristic monomer–monomer interactions within the FCP‐A tetramer are found between pigment–pigment and pigment–proteins at both stromal and lumenal sides . These interactions are different from those found in the dimeric FCP and trimeric LHCII, thus illustrating the different oligomerization states and high flexibility of the protein subunits in the LHC superfamily , which may be a result of adaptation to fulfill their roles for light harvesting and photoprotection in different light environments.…”

Section: Structures Of Fcp Subunits and Their Interactionsmentioning

confidence: 94%

“…According to the symmetry of their siliceous cell walls, diatoms are divided into two groups, namely pennate and centric groups. Genome and peptide analyses show that the pennate and centric diatoms have different composition of Lhcf subunits in thylakoid membranes . In the centric diatom Chaetoceros gracilis , FCPII exists mainly in three types designated FCP‐A, FCP‐B, and FCP‐C .…”

Section: Overall Structure Of the Psii–fcpii Supercomplexmentioning

confidence: 99%

“…The detailed binding environments of Chls c and Fxs in FCP are visualized from a higher resolution (1.8 Å) structure of an isolated FCP subunit from a pennate diatom P. tricornutum analyzed by X‐ray crystallography . This structure showed that the isolated FCP exists in a dimeric form (Fig.…”

Section: Overall Structure Of the Psii–fcpii Supercomplexmentioning

confidence: 99%

“…The main state of FCPIIs around PSII core is previously suggested as trimer based on the LHCII trimer structure of green lineage organisms . However, we isolated a homodimeric FCP from a pennate diatom Phaeodactylum tricornutum and solved its crystal structure at a high resolution . This dimer is encoded by an lhcf4 gene and may be one of the important forms of FCP existing around the PSII core.…”

Section: Introductionmentioning

confidence: 96%

“…In particular, single‐particle cryo‐EM analysis has provided a powerful tool to unravel the organization of several PSII–LHC and PSI–LHC supercomplexes from different lineages of organisms from cyanobacteria to green algae and higher plants . The structure of PSII–FCPII from diatoms, however, was solved only recently , and a high‐resolution crystal structure of an isolated FCP dimer was also reported recently . These studies revealed the oligomeric states and organization of FCPII subunits around the PSII core, the distribution and detailed binding environments of Chls c and Fxs, and a huge pigment–protein network within the PSII–FCPII supercomplex, which enable us to examine the excitation energy transfer and quenching pathways in the diatom PSII–FCPII in a greater detail.…”

Section: Introductionmentioning

confidence: 99%

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Structural features of the diatom photosystem II–light‐harvesting antenna complex

Wang

Zhao

et al. 2020

The FEBS Journal

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In photosynthesis, light energy is captured by pigments bound to light-harvesting antenna proteins (LHC) that then transfer the energy to the photosystem (PS) cores to initiate photochemical reactions. The LHC proteins surround the PS cores to form PS-LHC supercomplexes. In order to adapt to a wide range of light environments, photosynthetic organisms have developed a large variety of pigments and antenna proteins to utilize the light energy efficiently under different environments. Diatoms are a group of important eukaryotic algae and possess fucoxanthin (Fx) chlorophyll a/c proteins (FCP) as antenna which have exceptional capabilities of harvesting blue-green light under water and dissipate excess energy under strong light conditions. We have solved the structure of a PSII-FCPII supercomplex from a centric diatom Chaetoceros gracilis by cryo-electron microscopy, and also the structure of an isolated FCP dimer from a pennate diatom Phaeodactylum tricornutum by X-ray crystallography at a high resolution.These results revealed the oligomerization states of FCPs distinctly different from those of LHCII found in the green lineage organisms, the detailed binding patterns of Chl c and Fxs, a huge pigment network, and extensive protein-protein, pigment-protein, and pigment-pigment interactions within the PSII-FCPII supercomplex. These results therefore provide a solid structural basis for examining the detailed mechanisms of the highly efficient energy transfer and quenching processes in diatoms.

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Section: Structures Of Fcp Subunits and Their Interactionsmentioning

confidence: 94%

Section: Overall Structure Of the Psii–fcpii Supercomplexmentioning

confidence: 99%

Section: Overall Structure Of the Psii–fcpii Supercomplexmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 96%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Structural features of the diatom photosystem II–light‐harvesting antenna complex

Wang

Zhao

et al. 2020

The FEBS Journal

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Production and monitoring of biomass and fucoxanthin with brown microalgae under outdoor conditions

Gao

Sá

Itd

et al. 2020

Biotech & Bioengineering

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The effect of light on biomass and fucoxanthin (Fx) productivities was studied in two microalgae, Tisochrysis lutea and Phaeodactylum tricornutum. High and low biomass concentrations (1.1 and 0.4 g L−1) were tested in outdoor pilot‐scale flat‐panel photobioreactors at semi‐continuous cultivation mode. Fluorescence spectroscopy coupled with chemometric modeling was used to develop prediction models for Fx content and for biomass concentration to be applied for both microalgae species. Prediction models showed high R2 for cell concentration (.93) and Fx content (.77). Biomass productivity was lower for high biomass concentration than low biomass concentration, for both microalgae (1.1 g L−1: 75.66 and 98.14 mg L−1 d−1, for T. lutea and P. tricornutum, respectively; 0.4 g L−1: 129.9 and 158.47 mg L−1 d−1, T. lutea and P. tricornutum). The same trend was observed in Fx productivity (1.1 g L−1: 1.14 and 1.41 mg L−1 d−1, T. lutea and P. tricornutum; 0.4 g L−1: 2.09 and 1.73 mg L−1 d−1, T. lutea and P. tricornutum). These results show that biomass and Fx productivities can be set by controlling biomass concentration under outdoor conditions and can be predicted using fluorescence spectroscopy. This monitoring tool opens new possibilities for online process control and optimization.

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Isolation of fucoxanthin chlorophyll protein complexes of the centric diatom Thalassiosira pseudonana associated with the xanthophyll cycle enzyme diadinoxanthin de‐epoxidase

et al. 2022

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In the present study, low concentrations of the very mild detergent n‐dodecyl‐α‐d‐maltoside in conjunction with sucrose gradient ultracentrifugation were used to prepare fucoxanthin chlorophyll protein (FCP) complexes of the centric diatom Thalassiosira pseudonana. Two main FCP fractions were observed in the sucrose gradients, one in the upper part and one at high sucrose concentrations in the lower part of the gradient. The first fraction was dominated by the 18 kDa FCP protein band in SDS‐gels. Since this fraction also contained other protein bands, it was designated as fraction enriched in FCP‐A complex. The second fraction contained mainly the 21 kDa FCP band, which is typical for the FCP‐B complex. Determination of the lipid composition showed that both FCP fractions contained monogalactosyl diacylglycerol as the main lipid followed by the second galactolipid of the thylakoid membrane, namely digalactosyl diacylglycerol. The negatively charged lipids sulfoquinovosyl diacylglycerol and phosphatidyl glycerol were also present in both fractions in pronounced concentrations. With respect to the pigment composition, the fraction enriched in FCP‐A contained a higher amount of the xanthophyll cycle pigments diadinoxanthin (DD) and diatoxanthin (Dt), whereas the FCP‐B fraction was characterized by a lower ratio of xanthophyll cycle pigments to the light‐harvesting pigment fucoxanthin. Protein analysis by mass spectrometry revealed that in both FCP fractions the xanthophyll cycle enzyme diadinoxanthin de‐epoxidase (DDE) was present. In addition, the analysis showed an enrichment of DDE in the fraction enriched in FCP‐A but only a very low amount of DDE in the FCP‐B fraction. In‐vitro de‐epoxidation assays, employing the isolated FCP complexes, were characterized by an inefficient conversion of DD to Dt. However, in line with the heterogeneous DDE distribution, the fraction enriched in FCP‐A showed a more pronounced DD de‐epoxidation compared with the FCP‐B.

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Structural basis for blue-green light harvesting and energy dissipation in diatoms

Cited by 196 publications

References 63 publications

Structural features of the diatom photosystem II–light‐harvesting antenna complex

Structural features of the diatom photosystem II–light‐harvesting antenna complex

Production and monitoring of biomass and fucoxanthin with brown microalgae under outdoor conditions

Isolation of fucoxanthin chlorophyll protein complexes of the centric diatom Thalassiosira pseudonana associated with the xanthophyll cycle enzyme diadinoxanthin de‐epoxidase

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