Structural basis for BIR1-mediated negative regulation of plant immunity

Ma, Cuiyan; Liu, Yanan; Bai, Bing; Han, Zhifu; Tang, Jiao; Zhang, Heqiao; Yaghmaiean, Hoda; Zhang, Yuelin; Chai, Jijie

doi:10.1038/cr.2017.123

Cited by 42 publications

(45 citation statements)

References 10 publications

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“…Finally, our finding that expression of a oBIR3 -iSGN3 chimera could partially rescue the apoplastic barrier defects of the sgn3-3 mutant. Notably, BIR ectodomains specifically bind the ectodomains of SERKs (Ma et al, 2017;Hohmann et al, 2018a), while not forming complexes with the LRR ectodomain of the sequence-related NSP-INTERACTING KINASE1 (NIK1) ( Figure 6).…”

Section: Discussionmentioning

confidence: 99%

“…Recently, a constitutive, ligand-independent interaction between the LRR ectodomains of SERKs and of BAK1-INTERACTING RECEPTOR-LIKE KINASEs (BIRs) has been reported (Ma et al, 2017;Hohmann et al, 2018a). While BIR1 appears to have a catalytically active cytoplasmic kinase domain, BIR2-4 are receptor pseudokinases (Gao et al, 2009;Wang et al, 2011;Blaum et al, 2014).…”

Section: Recent Genetic Biochemical and Structural Evidence Suggest mentioning

confidence: 99%

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Constitutive activation of leucine-rich repeat receptor kinase signaling pathways by BAK1-interacting receptor-like kinase 3 chimera

Hohmann

Ramakrishna

Wang

et al. 2020

Preprint

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194 words) Receptor kinases with extracellular leucine-rich repeat domains (LRR-RKs) form the largest group of membrane signaling proteins in plants. LRR-RKs can sense small molecule, peptide or protein ligands, and may be activated by ligand-induced interaction with a shape complementary SOMATIC EMBRYOGENESIS RECEPTOR-LIKE KINASE (SERK) coreceptor kinase. We have previously shown that SERKs can also form constitutive, ligandindependent complexes with the LRR ectodomains of BAK1-interacting receptor-like kinase 3 (BIR3) receptor pseudokinases, negative regulators of LRR-RK signaling. Here we report that receptor chimaera in which the extracellular LRR domain of BIR3 is fused to the cytoplasmic kinase domains of the SERK-dependent LRR-RKs BRASSINOSTEROID INSENSITIVE1, HAESA and ERECTA form tight complexes with endogenous SERK co-receptors in the absence of ligand stimulus. Expression of these chimaera under the control of the endogenous promoter of the respective LRR-RK leads to strong gain-of-function brassinosteroid, floral abscission and stomatal patterning phenotypes, respectively. Importantly, a BIR3-GSO1/SGN3 chimera can partially complement sgn3 Casparian strip formation phenotypes, suggesting that GSO1/SGN3 receptor activation is also mediated by SERK proteins. Collectively, our protein engineering approach may be used to elucidate the physiological functions of orphan LRR-RKs and to identify their receptor activation mechanism in single transgenic lines. 2 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 MAP kinase signaling pathway (Meng et al., 2015) with diverse roles in plant development involvesthe LRR-RK ERECTA (ER) and its paralogs ERECTA-LIKE1 (ERL1) and ERL2 (Torii et al., 1996;Shpak, 2013). ER, ERL1 and ERL2 together control stomata development and their correct spacing on the leaf surface (Shpak et al., 2005). Cysteine-rich EPIDERMAL PATTERNING FACTOR peptides (EPFs) bind to the ectodomains of ER, ERL1 and ERL2 which form constitutive complexes with the ectodomain of the receptor-like protein (RLP) TOO MANY MOUTH (TMM) Vl and elution at 0.75 ml min -1 was monitored by ultraviolet absorbance at λ = 280 nm. To chimera; # numbers indicate independent lines.(C) Anti-GFP western blot together with the Ponceau-stained membrane as loading control.

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Section: Discussionmentioning

confidence: 99%

Section: Recent Genetic Biochemical and Structural Evidence Suggest mentioning

confidence: 99%

Constitutive activation of leucine-rich repeat receptor kinase signaling pathways by BAK1-interacting receptor-like kinase 3 chimera

Hohmann

Ramakrishna

Wang

et al. 2020

Preprint

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“…BAK1 interacts in vivo with the RLK BAK1-INTERACTING RECEPTOR-LIKE KINASE 1 (BIR1), a negative regulator of PTI responses and cell death pathways in Arabidopsis (Gao et al, 2009). It has been suggested that BIR1 sequesters BAK1 to prevent unwanted interactions with ligand-binding receptors in the absence of pathogens (Gao et al, 2009;Ma et al, 2017). Here, we study the role of BIR1 during viral infections and the molecular mechanisms whereby BIR1 is regulated.…”

Section: Introductionmentioning

confidence: 99%

The immune repressor BIR1 contributes to antiviral defense and undergoes transcriptional and post‐transcriptional regulation during viral infections

et al. 2019

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Summary BIR1 is a receptor‐like kinase that functions as a negative regulator of basal immunity and cell death in Arabidopsis. Using Arabidopsis thaliana and Tobacco rattle virus (TRV), we investigate the antiviral role of BIR1, the molecular mechanisms of BIR1 gene expression regulation during viral infections, and the effects of BIR1 overexpression on plant immunity and development. We found that SA acts as a signal molecule for BIR1 activation during infection. Inactivating mutations of BIR1 in the bir1‐1 mutant cause strong antiviral resistance independently of constitutive cell death or SA defense priming. BIR1 overexpression leads to severe developmental defects, cell death and premature death, which correlate with the constitutive activation of plant immune responses. Our findings suggest that BIR1 acts as a negative regulator of antiviral defense in plants, and indicate that RNA silencing contributes, alone or in conjunction with other regulatory mechanisms, to define a threshold expression for proper BIR1 function beyond which an autoimmune response may occur. This work provides novel mechanistic insights into the regulation of BIR1 homeostasis that may be common for other plant immune components.

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“…Future work should address how these potential novel interaction partners exert their influence on BR signalling mechanistically and how the interaction network can be integrated in an updated model of BR signalling. For example, the complexity of interactions revealed in the interactome analysis as well as in several other recent studies suggests that there is competition for binding between LRR-RLKs, modified by the presence of extracellular ligands and LRR-RLPs [158,165,[172][173][174].…”

Section: Signalling Integration At the Plasma Membranementioning

confidence: 99%

Deviating from the Beaten Track: New Twists in Brassinosteroid Receptor Function

Wolf¹

2020

IJMS

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A key feature of plants is their plastic development tailored to the environmental conditions. To integrate environmental signals with genetic growth regulatory programs, plants rely on a number of hormonal pathways, which are intimately connected at multiple levels. Brassinosteroids (BRs), a class of plant sterol hormones, are perceived by cell surface receptors and trigger responses instrumental in tailoring developmental programs to environmental cues. Arguably, BR signalling is one of the best-characterized plant signalling pathways, and the molecular composition of the core signal transduction cascade seems clear. However, BR research continues to reveal new twists to re-shape our view on this key signalling circuit. Here, exciting novel findings pointing to the plasma membrane as a key site for BR signalling modulation and integration with other pathways are reviewed and new inputs into the BR signalling pathway and emerging “non-canonical” functions of the BR receptor complex are highlighted. Together, this new evidence underscores the complexity of plant signalling integration and serves as a reminder that highly-interconnected signalling pathways frequently comprise non-linear aspects which are difficult to convey in classical conceptual models.

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Structural basis for BIR1-mediated negative regulation of plant immunity

Cited by 42 publications

References 10 publications

Constitutive activation of leucine-rich repeat receptor kinase signaling pathways by BAK1-interacting receptor-like kinase 3 chimera

Constitutive activation of leucine-rich repeat receptor kinase signaling pathways by BAK1-interacting receptor-like kinase 3 chimera

The immune repressor BIR1 contributes to antiviral defense and undergoes transcriptional and post‐transcriptional regulation during viral infections

Deviating from the Beaten Track: New Twists in Brassinosteroid Receptor Function

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