Structural and ultrastructural variations in in vitro and ex vitro rooting of microcuttings from two micropropagated Leucospermum (Proteaceae)

Suárez, Ernesto; Alfayate, Carmen; Pérez-Francés, J.F.; Rodríguez-Pérez, J.A.

doi:10.1016/j.scienta.2018.05.004

Cited by 7 publications

(2 citation statements)

References 53 publications

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“…At least 20 basal stem tissues (0.5 cm) were collected, with three replicates, for each time point from both control (non-IBA) and treated (IBA) plants to examine histological changes during AR formation at specific time points, i.e., 0, 12, 36, 48, and 72 h after excision (HAE). The collected tissues were fixed in formaldehyde acetic acid (FAA) solution (50% ethanol, 38% formaldehyde, 5% acetic acid) and treated as previously described [ 44 ]. Thin sections of 8 μm thickness were cut with a rotary microtome (LEICA RM2235) for histological observation.…”

Section: Methodsmentioning

confidence: 99%

Transcriptome Profiling Reveals Role of MicroRNAs and Their Targeted Genes during Adventitious Root Formation in Dark-Pretreated Micro-Shoot Cuttings of Tetraploid Robinia pseudoacacia L.

Uddin

Munir

Gull

et al. 2022

Genes

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Tetraploid Robinia pseudoacacia L. is a difficult-to-root species, and is vegetatively propagated through stem cuttings. Limited information is available regarding the adventitious root (AR) formation of dark-pretreated micro-shoot cuttings. Moreover, the role of specific miRNAs and their targeted genes during dark-pretreated AR formation under in vitro conditions has never been revealed. The dark pretreatment has successfully promoted and stimulated adventitious rooting signaling-related genes in tissue-cultured stem cuttings with the application of auxin (0.2 mg L−1 IBA). Histological analysis was performed for AR formation at 0, 12, 36, 48, and 72 h after excision (HAE) of the cuttings. The first histological events were observed at 36 HAE in the dark-pretreated cuttings; however, no cellular activities were observed in the control cuttings. In addition, the present study aimed to uncover the role of differentially expressed (DE) microRNAs (miRNAs) and their targeted genes during adventitious root formation using the lower portion (1–1.5 cm) of tetraploid R. pseudoacacia L. micro-shoot cuttings. The samples were analyzed using Illumina high-throughput sequencing technology for the identification of miRNAs at the mentioned time points. Seven DE miRNA libraries were constructed and sequenced. The DE number of 81, 162, 153, 154, 41, 9, and 77 miRNAs were upregulated, whereas 67, 98, 84, 116, 19, 16, and 93 miRNAs were downregulated in the following comparisons of the libraries: 0-vs-12, 0-vs-36, 0-vs-48, 0-vs-72, 12-vs-36, 36-vs-48, and 48-vs-72, respectively. Furthermore, we depicted an association between ten miRNAs (novel-m0778-3p, miR6135e.2-5p, miR477-3p, miR4416c-5p, miR946d, miR398b, miR389a-3p, novel m0068-5p, novel-m0650-3p, and novel-m0560-3p) and important target genes (auxin response factor-3, gretchen hagen-9, scarecrow-like-1, squamosa promoter-binding protein-like-12, small auxin upregulated RNA-70, binding protein-9, vacuolar invertase-1, starch synthase-3, sucrose synthase-3, probable starch synthase-3, cell wall invertase-4, and trehalose phosphatase synthase-5), all of which play a role in plant hormone signaling and starch and sucrose metabolism pathways. The quantitative polymerase chain reaction (qRT-PCR) was used to validate the relative expression of these miRNAs and their targeted genes. These results provide novel insights and a foundation for further studies to elucidate the molecular factors and processes controlling AR formation in woody plants.

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Section: Methodsmentioning

confidence: 99%

Transcriptome Profiling Reveals Role of MicroRNAs and Their Targeted Genes during Adventitious Root Formation in Dark-Pretreated Micro-Shoot Cuttings of Tetraploid Robinia pseudoacacia L.

Uddin

Munir

Gull

et al. 2022

Genes

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“…At least 20 hypocotyls were collected for each time point with three biological replicates. The collected tissues were fixed in formaldehyde acetic acid FAA (50% ethanol, 38% formaldehyde, acetic acid 5 mL/100 mL, and glycerol 5 mL/100 mL) and then treated with the protocol as described by [84]. A lower portion of the hypocotyl (8 µm) was prepared with a rotary microtome (LEICA RM2235) for histological study and then stained with safranin (1%) and fast-green (0.5%).…”

Section: Anatomical and Microscopic Analysismentioning

confidence: 99%

Transcriptomic and Anatomic Profiling Reveal Etiolation Promotes Adventitious Rooting by Exogenous Application of 1-Naphthalene Acetic Acid in Robinia pseudoacacia L.

Munir

Din

Imran

et al. 2021

Forests

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The process of etiolation contributes significantly to vegetative propagation and root formation of woody plants. However, the molecular interaction pattern of different factors for etiolated adventitious root development in woody plants remains unclear. In the present study, we explored the changes at different etiolation stages of adventitious root formation in Robinia pseudoacacia. Histological and transcriptomic analyses were performed for the etiolated lower portion of hypocotyls to ascertain the adventitious root responses. We found that the dark-treated hypocotyls formed roots earlier than the control. Exogenous application of NAA (0.3 mg/L) stimulated the expressions of about 310 genes. Among these, 155 were upregulated and 155 were downregulated. Moreover, differentially expressed genes (DEGs) were significantly enriched in multiple pathways, including the biosynthesis of secondary metabolites, metabolic pathway, plant hormone signal transduction, starch and sucrose metabolism, phenylpropanoid biosynthesis, and carbon metabolism. These pathways could play a significant role during adventitious root formation in etiolated hypocotyls. The findings of this study can provide novel insights and a foundation for further studies to elucidate the connection between etiolation and adventitious root formation in woody plants.

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Temporal profiling of physiological, histological, and transcriptomic dissection during auxin-induced adventitious root formation in tetraploid Robinia pseudoacacia micro-cuttings

Uddin,

Munir,

Larriba

et al. 2024

Planta

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Structural and ultrastructural variations in in vitro and ex vitro rooting of microcuttings from two micropropagated Leucospermum (Proteaceae)

Cited by 7 publications

References 53 publications

Transcriptome Profiling Reveals Role of MicroRNAs and Their Targeted Genes during Adventitious Root Formation in Dark-Pretreated Micro-Shoot Cuttings of Tetraploid Robinia pseudoacacia L.

Transcriptome Profiling Reveals Role of MicroRNAs and Their Targeted Genes during Adventitious Root Formation in Dark-Pretreated Micro-Shoot Cuttings of Tetraploid Robinia pseudoacacia L.

Transcriptomic and Anatomic Profiling Reveal Etiolation Promotes Adventitious Rooting by Exogenous Application of 1-Naphthalene Acetic Acid in Robinia pseudoacacia L.

Temporal profiling of physiological, histological, and transcriptomic dissection during auxin-induced adventitious root formation in tetraploid Robinia pseudoacacia micro-cuttings

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