Structural and Ultrastructural Localization of NGF and NGF Receptors in the Thymus of Subjects Affected by Myasthenia Gravis

Marinova, Ts.; Velikova, Kamelia; Petrov, Danail; Kŭtev, N; Stankulov, Ivan S.; Chaldakov, George N.; Triaca, Viviana; Manni, Luigi; Aloe, Luigi

doi:10.1080/08916930400016717

Cited by 13 publications

(16 citation statements)

References 18 publications

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“…The thymus is composed of different types of cells (thymic epithelial cells, macrophages and dendritic cells), and thymocytes at various stages of T-cell differentiation move from the cortical to the medullar area. Both mRNA and protein levels of p75NTR have been detected in the thymus of mammals [72,73,74] as well as the expression of TrkA, although different TrkA isoforms have been described in the thymus [75,76]. The expression of TrkA specifically characterizes rat and human thymocytes [77,78,79].…”

Section: Expression Of Ngf Receptors In the Immune Systemmentioning

confidence: 99%

NGF and Its Receptors in the Regulation of Inflammatory Response

Minnone

Benedetti

Bracci-Laudiero

2017

IJMS

224

163

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There is growing interest in the complex relationship between the nervous and immune systems and how its alteration can affect homeostasis and result in the development of inflammatory diseases. A key mediator in cross-talk between the two systems is nerve growth factor (NGF), which can influence both neuronal cell function and immune cell activity. The up-regulation of NGF described in inflamed tissues of many diseases can regulate innervation and neuronal activity of peripheral neurons, inducing the release of immune-active neuropeptides and neurotransmitters, but can also directly influence innate and adaptive immune responses. Expression of the NGF receptors tropomyosin receptor kinase A (TrkA) and p75 neurotrophin receptor (p75NTR) is dynamically regulated in immune cells, suggesting a varying requirement for NGF depending on their state of differentiation and functional activity. NGF has a variety of effects that can be either pro-inflammatory or anti-inflammatory. This apparent contradiction can be explained by considering NGF as part of an endogenous mechanism that, while activating immune responses, also activates pathways necessary to dampen the inflammatory response and limit tissue damage. Decreases in TrkA expression, such as that recently demonstrated in immune cells of arthritis patients, might prevent the activation by NGF of regulatory feed-back mechanisms, thus contributing to the development and maintenance of chronic inflammation.

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Section: Expression Of Ngf Receptors In the Immune Systemmentioning

confidence: 99%

NGF and Its Receptors in the Regulation of Inflammatory Response

Minnone

Benedetti

Bracci-Laudiero

2017

IJMS

224

163

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“…Our observations indicate the presence of HC with atypical localization – they were detected in the zones of tumor transformation. It is known that this is a finding characteristic of nerve growth factor‐associated hyperplasia (20), but we observed it in thymoma‐ associated myasthenia gravis as well.…”

Section: Discussionmentioning

confidence: 48%

Modulation of ABH histo‐blood group antigen expression in normal and myasthenic human thymus

Sarafian

Marinova

2006

APMIS

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The role of ABH histo-blood group antigens (HBGA) in intercellular communication during normal and pathological processes is still uncertain. The present work investigates the expression of ABH HBGA in epithelial cells and lymphocytes in normal thymus, and characterizes the modulation of their immunoreactivity during myasthenic transformation. Immunohistochemistry and immunoelectron microscopy were applied on normal young thymus and on myasthenia gravis-associated thymomas and thymic hyperplasias. The Hassall's corpuscules in the thymus of young individuals were homogeneously stained for HBGA, while in hyperplastic glands only their central part was positive. Stromal epithelial cells permanently expressed HBGA in all tissue samples. In thymomas, mainly the lymphocytes in close proximity to antigen expressing epithelial cells were positive, while in the hyperplastic gland the most intensely stained lymphocytes were those within Hassall's corpuscules. Novel evidence for modulation of ABH antigen reactivity in normal and myasthenic human thymus is presented. It suggests that HBGA might participate in the regulation of the cross-talk in the thymocyte microenvironment throughout the ontogeny, as well as during the myasthenic transformation.

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“…Using biochemical, molecular, immunohistochemical and immunogold electron microscope methodology we found that resident thymic cells, such as epithelial cells and mast cells of healthy subjects and of subjects affected by Myasthenia gravis are able to produce NGF [24]. We demonstrated that NGF and NGF receptors are overexpressed in the thymic cells, including mast cells of patients with Myasthenia gravis compared to control subjects [22]. While NGF and its receptors were also expressed in adult thymus, the immunocytochemical data showed an attenuated NGF and decreased NGFimmunopositivity of epithelial cell distribution during age-related involution [18].…”

Section: Discussionmentioning

confidence: 89%

“…Indirect immunoperoxidase method was carried out according to the protocols that we have previously described [5,11,22]. Briefly, serial cryostat sections from fresh frozen thymuses were immunostained with: Anti-NGF Ab, Anti-human MC Tryptase Ab, AntiTrkA Ab and Anti-Cytokeratin Ab (dilution 1:200, incubation time 30 min at room temperature).…”

Section: Microscopymentioning

confidence: 99%

Nerve Growth Factor Immunoreactivity of Mast Cells in Acute Involuted Human Thymus

Marinova¹,

Philipov²,

Aloe

2006

Inflammation

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The acute involution of the thymus is induced by either exogenous or endogenous factors, including some infections (infection type involution). The present study was focused on both detection and immunocytochemical analysis of NGF immunopositive mast cells in child thymus with acute infection-induced involution. Autopsy thymus specimens from children with infection diseases (Sepsis, Encephalomyelitis, Varicella) were examined at light and electron microscopic level and compared to normal infantile thymuses. We observed a redistribution of NGF immunopositive mast cells in infection-affected child thymus, which lobular architecture was collapsed. A positive correlation between the degree of the involutive changes, increased distribution and enhanced NGF immunoreactivity of mast cells was defined. The possible involvement of NGF immunopositive mast cells in the process of acute thymus involution is discussed.

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Structural and Ultrastructural Localization of NGF and NGF Receptors in the Thymus of Subjects Affected by Myasthenia Gravis

Cited by 13 publications

References 18 publications

NGF and Its Receptors in the Regulation of Inflammatory Response

NGF and Its Receptors in the Regulation of Inflammatory Response

Modulation of ABH histo‐blood group antigen expression in normal and myasthenic human thymus

Nerve Growth Factor Immunoreactivity of Mast Cells in Acute Involuted Human Thymus

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