Structural and thermodynamic features of complexes formed by DNA and synthetic polynucleotides with dodecylamine and dodecyltrimethylammonium bromide

Петров, А. И.; Khalil, Dayana; Kazaryan, R. L.; Savintsev, I. V.; Сухоруков, Б. И.

doi:10.1016/s1567-5394(02)00130-5

Cited by 36 publications

(23 citation statements)

References 47 publications

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“…Due to this property, pyrene is widely used in studies of membranes, micelles, and hydrophobic clusters [11,12]. Figure 1 (lower field) shows the dependence of the spectral parameter I 3 /I 1 , which is the most sensitive to hydrophobicity of microenvironment, on the concentration of cholenims at a constant DNA concentration (I 1 and I 3 are the amplitudes of oscillatory lines of emission spectra of the monomeric form of pyrene at 383 and 372 nm).…”

Section: Resultsmentioning

confidence: 99%

“…After dialyzing aqueous DNA solution (1.5 mg/ml) against 10 mM NaCl and 1 mM Tris-HCl (pH 7.2), its concentration was determined spectrophotometrically ( λ = 260 nm) using the molar extinction coefficient ε = 6600 M -1 cm -1 [11]. Melting temperature of DNA duplexes in buffer solution was 72°ë at a hyperchromic effect of 40%, indicating that the two-helix native structure of the duplexes was retained during sonication of genomic DNA.…”

Section: Methodsmentioning

confidence: 99%

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Lipoplexes based on cholesterol derivatives of oligo(ethylene propylene imines) in gene transfer In vitro and In vivo

Жданов

Богданенко

Петров

et al. 2005

Dokl Biochem Biophys

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The success of protocols of clinical genetic therapy depends on the presence of effective and safe vectorsviral [1][2][3] or nonviral [3][4][5][6]. Since nonviral vectors usually transfer genes much less effectively than viral vectors, the search for new effective nonviral vectors remains a topical problem [3,4,6]. The appearance of such vectors may increase the effectiveness of treating monogenic hereditary diseases and other pathologies by the methods of genetic and genetic-cellular therapy.This work is devoted to the study by physicochemical and cytological methods of the properties of lipoplexes-complexes between DNA and oligo(ethylene propylene imines), a novel group of lipid-like substances containing one, two, or three cholesterol molecules (cholenim I, II, and III, respectively), which were synthesized earlier [7,8], and the use of them for the transfer of functional genes into eukaryotic cells in vitro and in vivo. Lipoplexes based on these substances proved to be more suitable for targeted gene delivery than the ancestor of this class of transfection mediators-polyethylene imine [9, 10].It was shown that the effectiveness of transfection of monolayer cultures of eukaryotic cells in vivo with lipoplexes I, II, and III increases with an increase in the positive charge of particles and stability of lipoplexes and decrease in the hydrophobicity/hydrophilicity ratio of lipid, as well as depends on the size of particles and value of critical micelle concentration (CMC) of complexes. It was found that the distribution of lipoplexes injected into the hepatic portal vein of the mouse depends on cholenim structure. EXPERIMENTALAll reagents used in this study were of reagent grade. Organic solvents were distilled before use. Cholenims were synthesized as described earlier [7]. Cholenim I is tris-[2-N -(3-aminopropyl)aminoethyl]amine monocholesteroylformiate; cholenim II, tris-[2-N -(3-aminopropyl)aminoethyl]amine dicholesteroylformiate; and cholenim III, tris-[2-N -(3-aminopropyl)aminoethyl]amine tricholesteroylformiate.In the study, we used salmon roe genomic DNA (1.7 × 10 4 kDa) fragmented by mild sonication to duplexes with an average size of 4 kb. After dialyzing aqueous DNA solution (1.5 mg/ml) against 10 mM NaCl and 1 mM Tris-HCl (pH 7.2), its concentration was determined spectrophotometrically ( λ = 260 nm) using the molar extinction coefficient ε = 6600 M -1 cm -1 [11]. Melting temperature of DNA duplexes in buffer solution was 72°ë at a hyperchromic effect of 40%, indicating that the two-helix native structure of the duplexes was retained during sonication of genomic DNA. Melting curves of the complexes between genomic DNA fragments and cholenims were recorded on aVS4-2P spectrophotometer at a wavelength of 260 nm; the accuracy of measurements of temperature was ± 0.5 K . Pyrene fluorescence spectra [12] were recorded on an MPF-44B Perkin-Elmer spectrofluorometer. Circular dichroism spectra of the lipoplexes containing the pCMV-SPORT-β -G ‡l plasmid (BioLifeTech, catalogue no. 10586-04) and cholenims were...

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Lipoplexes based on cholesterol derivatives of oligo(ethylene propylene imines) in gene transfer In vitro and In vivo

Жданов

Богданенко

Петров

et al. 2005

Dokl Biochem Biophys

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“…The UV range CD-spectra is an effective mean to monitor the conformational transition of nucleic acids, the positive peak effect is related to base stacking, and the negative peak effect is related to helicity structure of nucleic acids [22,23]. As shown in the CD spectrum of DNA (displayed in Fig.…”

Section: Circular Dichroism Studiesmentioning

confidence: 99%

Detection of DNA hybridization by various spectroscopic methods using the copper tetraphenylporphyrin complex as a probe

Gong

Cai

et al. 2012

Microchim Acta

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We are presenting new and highly sensitive hybridization assays. They are based on various spectroscopic methods including resonance light scattering, circular dichroism, ultraviolet spectra and fluorescence spectra, as well as atomic force microscopy, and relies on the interaction of the Cu(II), Ni(II), Mg(II), Co(II), Cd(II), and Zn(II) complexes, respectively, of tetraphenylporphyrin (TPP) with double-strand DNA (dsDNA) and single strand DNA (ssDNA). The interaction results in amplified resonance light scattering (RLS) signals and enables the detection of hybridization without the need for labeling DNA. The RLS signals are strongest in case of the Cu (II)-TPP complex which therefore was selected as the probe. The technique is simple, robust, accurate, and can be completed in less than one hour.

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“…The increase in absorbance at λ > 320 nm with the increase in the concentration of P (AM-DMB) further proves that DNA interacted with P (AM-DMB), resulting from the formation of compact resonance light scattering particles. (35) The binding constant K was estimated according to the methods described. (37) Assuming that there is only one type of interaction between P (AM-DMB) and DNA in aqueous solution, equations (1) and (2) can be established:…”

Section: Ftir-spectramentioning

confidence: 99%

Spectral study of interaction between poly [acrylamide‐(2‐methyl acryloxyethyl) dimethyl buthylammonium bromide] and nucleic acids

2011

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A polycation, poly[acrylamide-(2-methylacryloxyethyl) dimethyl buthylammonium bromide] [P (AM-DMB)], was synthesized. The interaction between P (AM-DMB) and DNA was studied by spectral methods. Resonance light scattering spectra, FTIR-spectra and UV-spectra were used to study the mechanisms of the interaction. The results indicate that the conformation of the DNA alters during the interaction because the microenvironment of DNA changes. The binding constant K between P (AM-DMB) and DNA is 8.0 × 10(3) L mol(-1) .

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Structural and thermodynamic features of complexes formed by DNA and synthetic polynucleotides with dodecylamine and dodecyltrimethylammonium bromide

Cited by 36 publications

References 47 publications

Lipoplexes based on cholesterol derivatives of oligo(ethylene propylene imines) in gene transfer In vitro and In vivo

Lipoplexes based on cholesterol derivatives of oligo(ethylene propylene imines) in gene transfer In vitro and In vivo

Detection of DNA hybridization by various spectroscopic methods using the copper tetraphenylporphyrin complex as a probe

Spectral study of interaction between poly [acrylamide‐(2‐methyl acryloxyethyl) dimethyl buthylammonium bromide] and nucleic acids

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