Structural and spectroscopic study of a pectin isolated from citrus peel by using FTIR and FT-Raman spectra and DFT calculations

Bichara, Laura Cecilia; Álvarez, Patricia; Bimbi, María Victoria del Valle Fiori; Vaca, Hugo Rolando; Gervasi, C.A.; Brandán, Silvia Antonia

doi:10.1016/j.infrared.2016.03.009

Cited by 88 publications

(37 citation statements)

References 30 publications

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“…The spectral deconvolution of both IntegroPectins showed IR bands comparable in width and integrated area centered at ca. 1750 cm −1 deriving from esterified carboxylic groups of galacturonic acid (GalA) of the pectic polymers [10,25,[31][32][33][34]38]. This outcome suggests the presence of GalA within the two pectic polymers in a similar percentage.…”

Section: Resultsmentioning

confidence: 99%

“…Similarly, the commercial citrus pectin revealed a strong signal for the asymmetric -COO stretching (ca. 1610 cm −1 ; Table S1) of carboxylate groups within polygalacturonic acid [10,34,37,38], which was hidden, for the two IntegroPectins, by the large IR absorbance at 1594-1599 cm −1 (Table S1) attributable to aromatic skeleton vibrations [30,36,39,40]. Additional IR absorbance bands related to aromatic compounds, such as carotenoids and phenols [35,36,39,40], were observed in the 1520-1510 cm −1 region of lemon and grapefruit IntegroPectins.…”

Section: Resultsmentioning

confidence: 99%

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A New Water-Soluble Bactericidal Agent for the Treatment of Infections Caused by Gram-Positive and Gram-Negative Bacterial Strains

et al. 2020

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Grapefruit and lemon pectin obtained from the respective waste citrus peels via hydrodynamic cavitation in water only are powerful, broad-scope antimicrobials against Gram-negative and -positive bacteria. Dubbed IntegroPectin, these pectic polymers functionalized with citrus flavonoids and terpenes show superior antimicrobial activity when compared to commercial citrus pectin. Similar to commercial pectin, lemon IntegroPectin determined ca. 3-log reduction in Staphylococcus aureus cells, while an enhanced activity of commercial citrus pectin was detected in the case of Pseudomonas aeruginosa cells with a minimal bactericidal concentration (MBC) of 15 mg mL−1. Although grapefruit and lemon IntegroPectin share equal MBC in the case of P. aeruginosa cells, grapefruit IntegroPectin shows boosted activity upon exposure of S. aureus cells with a 40 mg mL−1 biopolymer concentration affording complete killing of the bacterial cells. Insights into the mechanism of action of these biocompatible antimicrobials and their effect on bacterial cells, at the morphological level, were obtained indirectly through Fourier Transform Infrared spectroscopy and directly through scanning electron microscopy. In the era of antimicrobial resistance, these results are of great societal and sanitary relevance since citrus IntegroPectin biomaterials are also devoid of cytotoxic activity, as already shown for lemon IntegroPectin, opening the route to the development of new medical treatments of polymicrobial infections unlikely to develop drug resistance.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

A New Water-Soluble Bactericidal Agent for the Treatment of Infections Caused by Gram-Positive and Gram-Negative Bacterial Strains

et al. 2020

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“…The analysis indicated a broad absorption peak at 3285 cm −1 corresponding to the hydroxyl (O-H) stretching vibration of polysaccharide as well as water absorption [42]. The displayed weak absorption band at 2936 cm −1 suggested the asymmetric vibration of (C-H) group [43]. The strong characteristic absorption peak at 1550 cm −1 was attributed to the vibration of COO − group of the galacturonic acid and those at 1399 cm −1 could correspond to ester carbonyl groups of the carboxylic function of GalA [42].…”

Section: Ftir Spectroscopy Of Pwpsmentioning

confidence: 99%

Prebiotic Activity of Poly- and Oligosaccharides Obtained from Plantago major L. Leaves

et al. 2020

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The aim of the present study was to evaluate the prebiotic potential of Plantago major L. leaves water-extractable polysaccharide (PWPs) and its lower molecular fractions. The structure of PWPs was investigated by high pressure anion exchange chromatography (HPAEC), size exclusion chromatography coupled with multi-angle laser light scattering detector (SEC-MALLS) and Fourier-transform infrared (FTIR) spectroscopy. The chemical composition and monosaccharide analyses showed that galacturonic acid was the main monosaccharide of PWPs followed by glucose, arabinose, galactose, rhamnose and xylose. FTIR study indicated a strong characteristic absorption peak at 1550 cm−1 corresponding to the vibration of COO− group of galacturonic acid. The PWPs was subjected to hydrolysis using commercial enzymes to obtain P. major low molecular fraction (PLM) which was successively separated by size exclusion chromatography on Biogel P2. PWPs and PLM were examined for in vitro prebiotic activity using various assays. Results gave evidence for changes in optical density of the bacteria cells and pH of the growth medium. A heterofermentative process with a lactate/acetate ratio ranged from 1:1 to 1:5 was observed. The ability of PLM to stimulate the production of certain probiotic bacteria glycohydrolases and to be fermented by Lactobacillus sp. strains was successfully proved.

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“…Plant cell walls make the skeleton of the plant body and differ in their chemical and structural properties depending on species, age, environmental conditions and also position within the same tree/plant [ 4 – 9 ]. The potential of CRM has been shown in many studies by revealing the spatial distribution of lignin [ 10 , 11 ] and other aromatic substances [ 12 ], the location of carbohydrates as pectin [ 13 , 14 ], the orientation of cellulose in the cell wall [ 15 ], conformational changes of proteins [ 16 ], the accumulation of fats and waxes of the plant cuticle [ 12 , 17 ] and also minerals [ 18 , 19 ]. An extensive practical guide on Raman imaging of plant cell walls can be found in [ 20 ].…”

Section: Introductionmentioning

confidence: 99%

Multivariate unmixing approaches on Raman images of plant cell walls: new insights or overinterpretation of results?

et al. 2018

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BackgroundPlant cell walls are nanocomposites based on cellulose microfibrils embedded in a matrix of polysaccharides and aromatic polymers. They are optimized for different functions (e.g. mechanical stability) by changing cell form, cell wall thickness and composition. To reveal the composition of plant tissues in a non-destructive way on the microscale, Raman imaging has become an important tool. Thousands of Raman spectra are acquired, each one being a spatially resolved molecular fingerprint of the plant cell wall. Nevertheless, due to the multicomponent nature of plant cell walls, many bands are overlapping and classical band integration approaches often not suitable for imaging. Multivariate data analysing approaches have a high potential as the whole wavenumber region of all thousands of spectra is analysed at once.ResultsThree multivariate unmixing algorithms, vertex component analysis, non-negative matrix factorization and multivariate curve resolution–alternating least squares were applied to find the purest components within datasets acquired from micro-sections of spruce wood and Arabidopsis. With all three approaches different cell wall layers (including tiny S1 and S3 with 0.09–0.14 µm thickness) and cell contents were distinguished and endmember spectra with a good signal to noise ratio extracted. Baseline correction influences the results obtained in all methods as well as the way in which algorithm extracts components, i.e. prioritizing the extraction of positive endmembers by sequential orthogonal projections in VCA or performing a simultaneous extraction of non-negative components aiming at explaining the maximum variance in NMF and MCR-ALS. Other constraints applied (e.g. closure in VCA) or a previous principal component analysis filtering step in MCR-ALS also contribute to the differences obtained. ConclusionsVCA is recommended as a good preliminary approach, since it is fast, does not require setting many input parameters and the endmember spectra result in good approximations of the raw data. Yet the endmember spectra are more correlated and mixed than those retrieved by NMF and MCR-ALS methods. The latter two give the best model statistics (with lower lack of fit in the models), but care has to be taken about overestimating the rank as it can lead to artificial shapes due to peak splitting or inverted bands. Electronic supplementary materialThe online version of this article (10.1186/s13007-018-0320-9) contains supplementary material, which is available to authorized users.

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Structural and spectroscopic study of a pectin isolated from citrus peel by using FTIR and FT-Raman spectra and DFT calculations

Cited by 88 publications

References 30 publications

A New Water-Soluble Bactericidal Agent for the Treatment of Infections Caused by Gram-Positive and Gram-Negative Bacterial Strains

A New Water-Soluble Bactericidal Agent for the Treatment of Infections Caused by Gram-Positive and Gram-Negative Bacterial Strains

Prebiotic Activity of Poly- and Oligosaccharides Obtained from Plantago major L. Leaves

Multivariate unmixing approaches on Raman images of plant cell walls: new insights or overinterpretation of results?

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