Structural and nonstructural proteins of a rabbit parvovirus

Abstract

The structural and nonstructural polypeptides of a rabbit parvovirus (RPV) (F-7-9 strain) were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The virion contained three polypeptide components, A (molecular weight, 96,000), B (85,000), and C (75,000). A part of the polypeptide C was cleaved into the smaller-molecular-weight polypeptide C' by proteolysis during purification steps. The major polypeptide C together with C' constituted about 87% of the total viral proteins, and the minor polypeptides, A and B, constituted 4 and 9%, respectively. The structural polypeptides of empty particles were similar in size and composition to those of the virion, but the content of the C' polypeptide was very low. When rabbit kidney cell cultures were infected with RPV, the C polypeptide was detected as early as 15 h postinfection, whereas A and B were first demonstrated at 18 h. The C' polypeptide was not detected for 44 h. In addition to the three structural polypeptides, at least three nonstructural polypeptides, E, F, and G, were demonstrated in the RPV-infected cells. Polypeptide E (molecular weight, 49,000), detected mostly in cytoplasm, seemed to be a cellular protein. The F (25,000) and G (22,000) polypeptides seemed to be virus-coded proteins since they were precipitated with the anti-RPV rabbit immunoglobulin. According to partial proteolysis and peptide mapping, the F and G polypeptides shared the same peptide components.

Identification and characterization of a porcine parvovirus nonstructural polypeptide

“…Virus-specific antiserum was used in the identification of two forms of a nonstructural protein (84 and 94 kDa) from H-1 parvovirus-infected cells (18). Viral noncapsid polypeptides have also been described in Kilham rat virus-infected (15), rabbit parvovirus-infected (13), and Aleutian disease virus-infected (2) cells.…”

Characterization of capsid and noncapsid proteins of B19 parvovirus propagated in human erythroid bone marrow cell cultures

“…B19 capsid proteins of 58 and 84 kDa have been detected by immunoblotting in the sera of infected patients (N. Young and K. Ozawa, in J. R. Pattison, ed., Parvoviruses and Human 0 20 40 Disease in press) and fetal tissue of a spontaneous abortus (4). These proteins are similar in number, size, and relative proportions to capsid proteins of other animal parvoviruses (7,8,12,17,18). In particular, the major capsid species (VP2) weighed 58 kDa, which is similar to those of the dominant capsid proteins of several adeno-associated viruses, feline panleucopenia virus, LullI virus, minute virus of mice, and Kilham rat virus.…”

Viral Diseases

“…The virus had the morphological, physical, and chemical properties of a parvovirus. The various proteins of the virus have been characterized (Matsunaga and Matsuno, 1983). Subsequently, parvovirus was recovered from kidney cells of neonatal rabbits in the United States (Metcalf et al, 1989).…”