Structural and morphological transition of long-chain phospholipid vesicles induced by mixing with short-chain phospholipid

Takajo, Yuichi; Matsuki, Hitoshi; Matsubara, Hiroki; Tsuchiya, Koji; Aratono, Makoto; Yamanaka, Masanori

doi:10.1016/j.colsurfb.2009.12.025

Cited by 20 publications

(25 citation statements)

References 35 publications

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“…Bicelles are lipid nanostructures formed by a long and a short chain phospholipid molecule dispersed in an aqueous solution [1,2]. These aggregates have been used up until now as membrane models for nuclear magnetic resonance (NMR) structural studies of membrane biomolecules [3].…”

Section: Introductionmentioning

confidence: 99%

Bicellar systems as modifiers of skin lipid structure

Rodríguez

Barbosa-Barros

Rubió

et al. 2011

Colloids and Surfaces B: Biointerfaces

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Section: Introductionmentioning

confidence: 99%

Bicellar systems as modifiers of skin lipid structure

Rodríguez

Barbosa-Barros

Rubió

et al. 2011

Colloids and Surfaces B: Biointerfaces

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“…The results of the GP 340 analysis were also helpful to identify the bicelle and micelle of DMPC/DHPC assemblies at total lipid concentrations less than 20 mM. The coexistence of DHPC could disturb the ordered properties of the DMPC vesicle [38]. As compared to the pure DMPC vesicle, a slight increase in GP 340 values was observed for DMPC/DHPC assemblies at X DHPC of 0.33 or 0.4, suggesting more ordered (tightly packed) states of these membranes.…”

Section: Membrane Polarity (Gp 340 )mentioning

confidence: 99%

Systematic Characterization of DMPC/DHPC Self-Assemblies and Their Phase Behaviors in Aqueous Solution

Taguchi

Suga

Hayashi

et al. 2018

Colloids and Interfaces

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Self-assemblies composed of 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) and 1,2-dihexanoyl-sn-glycero-3-phosphocholine (DHPC) form several kinds of structures, such as vesicle, micelle, and bicelle. Their morphological properties have been studied widely, but their interfacial membrane properties have not been adequately investigated. Herein, we report a systematic characterization of DMPC/DHPC assemblies at 20 °C. To investigate the phase behavior, optical density OD500, size (by dynamic light scattering), membrane fluidity 1/PDPH (using 1,6-diphenyl-1,3,5-hexatriene), and membrane polarity GP340 (using 6-dodecanoyl-N,N-dimethyl-2-naphthylamine) were measured as a function of molar ratio of DHPC (XDHPC). Based on structural properties (OD500 and size), large and small assemblies were categorized into Region (i) (XDHPC < 0.4) and Region (ii) (XDHPC ≥ 0.4), respectively. The DMPC/DHPC assemblies with 0.33 ≤ XDHPC ≤ 0.67 (Region (ii-1)) showed gel-phase-like interfacial membrane properties, whereas DHPC-rich assemblies (XDHPC ≥ 0.77) showed disordered membrane properties (Region (ii-2)). Considering the structural and interfacial membrane properties, the DMPC/DHPC assemblies in Regions (i), (ii-1), and (ii-2) can be determined to be vesicle, bicelle, and micelle, respectively.

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“…4a), in which a part of bilayers transformed to multilamellar vesicles [7,9]. Takajo et al reported the effect of DHPC that can destabilize DMPC bilayer [16]. Therefore, the morphological stability of the DMPC bilayer can be affected by the existence of DHPC.…”

Section: Effect Of Dilution On Structural Propertiesmentioning

confidence: 99%

“…In aqueous solution, long-chained saturated phospholipids (e.g., 1,2-dimyristoyl-snglycero-3-phosphocholine (DMPC, C14:0); melting temperature Tm = 23 ºC) often form bilayer membranes, that finally construct spherical vesicles due to the thermodynamic stability of the bilayers [6]. When short-chained phospholipid 1,2-dihexanoyl-sn-glycero-3-phosphocholine (DHPC, C6:0) is excessively added to a DMPC suspensions, they form micelle-like small assemblies at the total lipid concentration above the critical micelle concentration (CMC): for DHPC, the CMC is 15 mM [7,8]), in which the small bilayer morphology is also maintained [7,[9][10][11][12][13][14][15][16]. The morphology of small self-assemblies, such as micelle and bilayer-micelle (bicelle), can be dependent on the total lipid concentration, and on the lipid mixing ratio in lipid binary system [7].…”

Section: Introductionmentioning

confidence: 99%

Morphological Estimation of DMPC/DHPC Self-Assemblies in Diluted Condition: Based on Physicochemical Membrane Properties

Taguchi¹,

Suga²,

Hayashi³

et al. 2018

Preprint

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Self-assembly membranes, composed of 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) and 1,2-dihexanoyl-sn-glycero-3-phosphocholine (DHPC), were characterized at the total lipid concentration below 20 mM. The sizes of the assemblies varied depending on the molar ratio of DMPC and DHPC (q = [DMPC]/[DHPC]). The small assemblies with diameter of ca. 10 nm were formed at q ≤ 2.0 at 20 ºC (below phase transition temperature of DMPC). The physicochemical membrane properties were then studied using fluorescence probes, 1,6-diphenyl-1,3,5-hexatriene and 6-dodecanoyl-N,N-dimethyl-2-naphthylamine, upon the dilution. DHPC micelle showed a higher membrane fluidity, while the DMPC/DHPC membranes at q ≥ 0.5 showed lower membrane fluidities as well as DMPC vesicle in gel (ordered) phase. Upon dilution, the ordered membrane properties were maintained while the solution turbidities increased, implying the morphological change of the self-assembly, bicelle to the vesicle in gel phase. Based on the obtained results, a phase diagram of DMPC/DHPC binary system (at 20 ºC) is described: (i) the bicelle suspension is transparent and the membrane is in ordered state, (ii) the micelle suspension is transparent and the membrane is in disordered state, (iii) the vesicle suspension is turbid and the membrane is in ordered state.

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Structural and morphological transition of long-chain phospholipid vesicles induced by mixing with short-chain phospholipid

Cited by 20 publications

References 35 publications

Bicellar systems as modifiers of skin lipid structure

Bicellar systems as modifiers of skin lipid structure

Systematic Characterization of DMPC/DHPC Self-Assemblies and Their Phase Behaviors in Aqueous Solution

Morphological Estimation of DMPC/DHPC Self-Assemblies in Diluted Condition: Based on Physicochemical Membrane Properties

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