Structural and morphologic evaluation of a novel detergent–enzymatic tissue-engineered tracheal tubular matrix

Jungebluth, Philipp; Go, Tetsuhiko; Asnaghi, M. Adelaide; Bellini, Silvia; Martorell, Jaume; Calore, Chiara; Urbani, Luca; Ostertag, Helmut; Mantero, Sara; Conconi, Maria Teresa; Macchiarini, Paolo

doi:10.1016/j.jtcvs.2008.09.085

Cited by 118 publications

(128 citation statements)

References 18 publications

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“…Each protocol was performed for 8h and 24h to analyze the influence of decellularization time. Light gray boxes refer to control of reactions (1,2,7,8); dark gray boxes refer to the employed protocols (3 to 6 and 9 to 12). Painted boxes represent methods that were employed during these protocols.…”

Section: Second Set Of Decellularization Protocols (Led Based Methods)mentioning

confidence: 99%

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Light-emitting diode effects on combined decellularization of tracheae. A novel approach to obtain biological scaffolds

et al. 2014

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PURPOSE:To obtain a decellularized tracheal scaffold associating traditional approaches with the novel light-emitting diode (LED) proposal. METHODS:This study was performed with New Zealand adult rabbits weighing 3.0 -4.0 kg. Different protocols (22) were used combining physical (agitation and LED irradiation), chemical (SDS and Triton X-100 detergents), and enzymatic methods (DNase and RNase). RESULTS:Generally, the cells surrounding soft tissues were successfully removed, but none protocol removed cells from the tracheal cartilage. However, longer protocols were more effective. The cost-benefits relation of the enzymatic processes was not favorable. It was possible to find out that the cartilaginous tissue submitted to the irradiation with LED 630nm and 475 nm showed an increased number of gaps without cells, but several cells were observed to be still present. CONCLUSION:The light-emitting diode is a promising tool for decellularization of soft tissues.

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Section: Second Set Of Decellularization Protocols (Led Based Methods)mentioning

confidence: 99%

“…Tracheal lesions whose length is more than 50% (around 6 cm) in adults and a third of the trachea in small children require curative treatment [1][2][3][4][5][6][7][8] . In many cases, endoluminal therapies followed by surgical procedures are required, resulting in high costs to healthcare systems 4,9 .…”

Section: Introductionmentioning

confidence: 99%

Light-emitting diode effects on combined decellularization of tracheae. A novel approach to obtain biological scaffolds

et al. 2014

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“…Decellularized aortic grafts [147], jejunum [148,149,150], bladder [151], and trachea [151,152,153,146] …”

Section: Decellularized Tissuementioning

confidence: 99%

“…Heterotopic grafts are useful for verification of biological and immunologic compatibility, however, their predictive ability for orthotopic implantation is limited. Other studies have employed orthotopic implantation, but have tested only a single experimental group, often controlling for in vitro cell seeding rather than processing conditions [151,152,153]. Of those, many were window patches which have increased survival compared to full circumference grafts, but fail to address the underlying clinical need.…”

Section: Acknowledgementsmentioning

confidence: 99%

Tracheal Tissue Engineering

Birla

2014

Introduction to Tissue Engineering

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“…Entre las escasas opciones para tratar a estos pacientes está la resección traqueal; sin embargo, dicho tratamiento solo se puede utilizar cuando el segmento de tráquea que se va a remover no excede el 30 % de la longitud total del órgano, lo cual constituye una importante limitación puesto que existen traumas o enfermedades que afectan un porcentaje mayor de tejido (5). Por tanto, se han desarrollado diferentes alternativas terapéuticas para atender estas necesidades.…”

Section: Introductionunclassified

Evaluación del método químico-enzimático de descelularización para la obtención de matrices extracelulares de tráquea en el modelo porcino

Morales-Valencia

Patiño-Vargas

Correa-Londoño³

et al. 2016

iatreia

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Introducción: diferentes enfermedades afectan la tráquea y deterioran la calidad de vida. La ingeniería tisular es una alternativa terapéutica para los pacientes con esas enfermedades: matrices de tráquea descelularizadas y sembradas con células del receptor no generan respuesta inmune y pueden prevenir incluso el rechazo de zootrasplantes.Objetivo: evaluar un método de descelularización para obtener matrices extracelulares de tráquea en el modelo porcino.Materiales y métodos: a partir de 5 tráqueas porcinas se formaron dos grupos de estudio, controles y tratados con un método químico-enzimático. Se hizo análisis histológico con hematoxilina-eosina, coloración tricrómica de Masson y safranina O. Se evaluaron las propiedades biomecánicas de ambos grupos, mediante la determinación del módulo de Young, la fuerza máxima y el porcentaje de deformación.Resultados: en las muestras tratadas se observó una disminución del 66 % del contenido celular en comparación con los controles. Se preservó el colágeno y se detectó reducción de los glucosaminoglucanos. Las pruebas biomecánicas revelaron una diferencia estadís-ticamente significativa del porcentaje de deformación, sin alteración de los demás pará-metros.Conclusiones: el método evaluado demostró ser eficiente para descelularizar tráqueas de cerdo, con una disminución importante en el costo y el tiempo de tratamiento, por lo que podría ser una buena opción en las condiciones socioeconómicas de Colombia. Introduction: Different diseases may affect the trachea and, therefore, the quality of life. Tissue engineering may be a therapeutic alternative for patients with such diseases, using decellularized trachea matrixes seeded with cells from the recipient, which do not generate immune response and may even prevent rejection of zoo-transplants.Objective: To evaluate a decellularization method to obtain tracheal extracellular matrixes in the porcine model. Materials and methods:Two study groups, treated and control, were obtained from 5 porcine tracheas. A chemical-enzymatic method for decellularization was used. Histological analyses were performed with hematoxylin-eosin, Masson's trichromic stain and safranin O. Biomechanical properties of both groups were evaluated by determining the Young modulus, maximum strength and deformation rate.Results: Compared to the controls, there was a 66 % decrease in the cell content in the treated specimens. Collagen was preserved and a reduction of glycosaminoglycans was detected. Biomechanical tests revealed a significant difference in the percentage of deformation, with no alteration of the remaining parameters. Conclusions:The evaluated decellularization method proved to be efficient to reduce the cellular content of porcine tracheas, with a considerable decrease in cost and production time. These advantages could make it a good option for the socio-economic Colombian conditions. KEY WORDSDecellularized Matrix; Recellularized Matrix; Regeneration, Airway; Trachea; Tissue Engineering RESUMOAvaliação do método químico-enzimático de descelu...

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Structural and morphologic evaluation of a novel detergent–enzymatic tissue-engineered tracheal tubular matrix

Abstract: Bioengineered tracheal matrices displayed similar structural and mechanical characteristics to native tracheas and excite no immune response to 30 days when implanted as allografts or xenografts. This method holds great promise for the future of tissue-engineered airway replacement.

Cited by 118 publications

References 18 publications

Light-emitting diode effects on combined decellularization of tracheae. A novel approach to obtain biological scaffolds

Light-emitting diode effects on combined decellularization of tracheae. A novel approach to obtain biological scaffolds

Tracheal Tissue Engineering

Evaluación del método químico-enzimático de descelularización para la obtención de matrices extracelulares de tráquea en el modelo porcino

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