Structural and molecular pathology of the atrium in boxer arrhythmogenic right ventricular cardiomyopathy

Vila, Jorge; Pariaut, Romain; Moı̈se, N. Sydney; Oxford, Eva M.; Fox, Philip R.; Reynolds, Caryn; Saelinger, Carley

doi:10.1016/j.jvc.2016.09.001

Cited by 17 publications

(14 citation statements)

References 39 publications

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“…In comparison with other species, canine CDCs exhibit remarkable phenotypic similarities to mouse, rat, human, and porcine cells . As we elucidated in the introduction, canines are a unique naturally occurring model for non‐ischemic cardiomyopathy in humans, specifically ARVC . The effect of cryopreservation on human CDCs is poorly understood; therefore given the similarities with canine cells the present study also provides vital translational information to the human field.…”

Section: Discussionmentioning

confidence: 84%

“…Two distinct histopathological variations of canine DCM have been described; attenuated wavy fiber type and fibro‐fatty infiltration type . Fibro‐fatty infiltration is considered analogous to arrhythmogenic right ventricular cardiomyopathy (ARVC) in humans because of the comparable pathological changes and clinical presentation between the two species . Although the pathophysiologic mechanism underlying ARVC remains unclear, it is thought to include molecular pathways involved in the formation of mechanical and electrical coupling, apoptosis, and migration and differentiation of epicardial‐derived cells .…”

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confidence: 99%

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Cryopreservation of canine cardiosphere‐derived cells: Implications for clinical application

et al. 2017

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The clinical application of cardiosphere-derived cells (CDCs) to treat cardiac disease has gained increasing interest over the past decade. Recent clinical trials confirm their regenerative capabilities, although much remains to be elucidated about their basic biology. To develop this new treatment modality, in a cost effective and standardized workflow, necessitates the creation of cryopreserved cell lines to facilitate access for cardiac patients requiring urgent therapy. Cryopreservation may however lead to alterations in cell behavior and potency. The aim of this study was to investigate the effect of cryopreservation on canine CDCs. CDCs and mesenchymal stem cells (MSCs) isolated from five dogs were characterized. CDCs demonstrated a population doubling time that was unchanged by cryopreservation (fresh vs. cryopreserved; 57.13 6 5.27 h vs. 48.94 6 9.55 h, P 5 0.71). This was slower than for MSCs (30.46 h, P < 0.05). The ability to form clones, self-renew, and commit to multiple lineages was unaffected by cryopreservation. Cryopreserved CDCs formed larger cardiospheres compared to fresh cells (P < 0.0001). Fresh CDCs showed a high proportion of CD105 1 (89.0% 6 4.98) and CD44 1 (99.68% 6 0.13) cells with varying proportions of CD90 1 (23.36% 6 9.78), CD34 1 (7.18% 6 4.03) and c-Kit 1 (13.17% 6 8.67) cells. CD45 1 (0.015% 6 0.005) and CD29 1 (2.92% 6 2.46) populations were negligible. Increasing passage number of fresh CDCs correlated with an increase in the proportion of CD34 1 and a decrease in CD90 1 cells (P 5 0.003 and 0.03, respectively). Cryopreserved CDCs displayed increased CD34 1 (P < 0.001) and decreased CD90 1 cells (P 5 0.042) when compared to fresh cells. Overall, our study shows that cryopreservation of canine CDCs is feasible without altering their stem characteristics, thereby facilitating their utilization for clinical trials. V C 2017 International Society for Advancement of Cytometry

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Section: Discussionmentioning

confidence: 84%

mentioning

confidence: 99%

Cryopreservation of canine cardiosphere‐derived cells: Implications for clinical application

et al. 2017

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“…Desmosomes are found throughout the cardiac myocardium, including atrial myocardium [16]. There are anecdotal reports documenting atrial myocardial involvement in ARVC patients based on postmortem case series [17] and supported by animal studies [8,18]. The evidence of altered electrical conduction within the atria of ARVC patients has also previously been demonstrated [19].…”

Section: Pathophysiologic Mechanisms Of Af In Arvc Patientsmentioning

confidence: 88%

“…Left ventricular (LV) involvement in the disease leading to systolic dysfunction may also be associated with complications such as AF and thromboembolic events [2]. On the other hand, it has been suggested that development of atrial myocardial substrate in ARVC may be driven by genetically determined desmosomal dysfunction [8]. It is possible that involvement of atrial myocardium leading to atrial dilatation [5] and AF is caused by the same mechanisms as fibrotic replacement of myocardium in the right ventricular walls and takes place in a parallel with involvement of ventricular myocardium.…”

Section: Introductionmentioning

confidence: 99%

Atrial fibrillation as a clinical characteristic of arrhythmogenic right ventricular cardiomyopathy: Experience from the Nordic ARVC Registry

Baturova

Haugaa

Jensen

et al. 2020

International Journal of Cardiology

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Background: Recent studies in arrhythmogenic right ventricular cardiomyopathy (ARVC) patients have drawn attention to atrial fibrillation (AF) as an arrhythmic manifestation of ARVC and as an indicator of atrial involvement in the disease progression. We aimed to assess the prevalence of AF in the Scandinavian cohort of ARVC patients and to evaluate its association with disease clinical manifestations. Methods: Study sample comprised of 293 definite ARVC patients by 2010 Task Force criteria (TFC2010) and 141 genotype-positive family members (total n = 434, 43% females, median age at ARVC diagnosis 41 years [interquartile range (IQR) 28-52 years]). ARVC diagnostic score was calculated as the sum of major (2 points) and minor (1 point) criteria in all categories of the TFC2010. Results: AF was diagnosed in 42 patients (10%): in 41 patients with definite ARVC diagnosis (14%) vs in one genotype-positive family member (1%), p b 0.001. The median age at AF onset was 51 (IQR 38-58) years. The prevalence of AF was related to the ARVC diagnostic score: it significantly increased starting with the diagnostic score 4 (2% in those with score 3 vs 13% in those with score 4, p = 0.023) and increased further with increased diagnostic score (Somer's d value is 0.074, p b 0.001). Conclusion: AF is seen in 14% of definite ARVC patients and is related to the severity of disease phenotype thus suggesting AF being an arrhythmic manifestation of this cardiomyopathy indicating atrial myocardial involvement in the disease progression.

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“…10,11 An inherited ventricular arrhythmia has been identified in some German Shepherds. It increases phase 4 originating from the left side and supraventricular arrhythmias in these dogs.…”

Section: Noncardiac Causes Of Ventricular Tachycardiamentioning

confidence: 99%

Ventricular Tachyarrhythmias

Pariaut¹

2015

Small Animal Critical Care Medicine

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Structural and molecular pathology of the atrium in boxer arrhythmogenic right ventricular cardiomyopathy

Cited by 17 publications

References 39 publications

Cryopreservation of canine cardiosphere‐derived cells: Implications for clinical application

Cryopreservation of canine cardiosphere‐derived cells: Implications for clinical application

Atrial fibrillation as a clinical characteristic of arrhythmogenic right ventricular cardiomyopathy: Experience from the Nordic ARVC Registry

Ventricular Tachyarrhythmias

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