Structural and Mixture Analysis of Glycerophosphoric Acid Derivatives by Fast Atom Bombardment. Tandem Mass Spectrometry

Fast atom bombardment mass spectrometry of phospholipids

“…The collision induced decomposition of [M -151or the other negative ions at high mass ([M-60]-or [M -86]-) from GPC lipids yield quite abundant carboxylate anions corresponding to the substituents at sn-1 and sn-2 (36, 38,40). This can be seen in Fig.…”

“…3c). The abundant formation of this ion has been used as a basis for the characterization of specific FAB ions, which are derived from glycerophosphocholine lipids present in crude mixtures of phospholipids (31 38,40). This can be seen in Fig.…”

A universal product ion nomenclature for [M−H]−, [M+H]+ and [M+nNa−(n−1)H]+ (n=1–3) glycerophospholipid precursor ions based on high-energy CID by MALDI-TOF/RTOF mass spectrometry

“…Although several groups attempted to establish for a limited number of fragment ions obtained by negative-ion FAB-MS and/or product ions based high-energy CID in combination with tandem mass spectrometry (MS/MS) a suggested fragment/product ion nomenclature was never widely used [42][43][44][45][46][47][48][49]. Alternatively, Hsu et al investigated the fragmentation behavior of deprotonated, protonated as well as (multiply) lithiated precursor ions of various classes of glycerophospholipids in detail by low-energy CID utilizing either a triple quadrupole or an ion trap instrument combined with ESI but without postulating a product ion nomenclature based on common fragmentation rules [50][51][52][53][54][55][56][57][58].…”

Characterization of Diacylglycerylphosphocholine Molecular Species by FAB-CAD-MS/MS: A General Method Not Sensitive to the Nature of the Fatty Acyl Groups

“…Because the parent ion is equivalent to the phosphatidic acid ion, this technique should be equally applicable to all other phospholipid classes where this fragment ion is present in the spectrum. (J Am Soc Mass Spectrom 1992, 3, 71-78) P ast atom bombardment-mass spectrometry (FAB-MS) and FAB-collision-activated dissociation-tandem mass spectrometry (FAB-CAD-MS/MS) techniques have been developed to characterize the polar head group and fatty acyl composition of a variety of glycerylphospholipid classes [1][2][3][4][5][6]. In the case of phosphatidylcholine (PC) derivatives (Figure 1 that this could be used to establish the relative position of the two different fatty acyl moieties in a PC compound.…”