Structural and genetic characterization of the colitose-containing O-specific polysaccharide from the lipopolysaccharide of Herbaspirillum frisingense GSF30T

Velichko, Natalya S.; Kokoulin, Maxim S.; Sigida, Elena N.; Kuchur, P. D.; Komissarov, Aleksey; Kovtunov, E. A.; Fedonenko, Yu. P.

doi:10.1016/j.ijbiomac.2020.06.093

Cited by 6 publications

(1 citation statement)

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“…A feature of the high relevance for interaction with the host is the type of exopolysaccharide (EPS) produced. In H. frisingense GSF30, the probable O-antigen biosynthesis locus (OGC) has been identified and includes genes related to colitis synthesis [ 37 ]. In H. frisingense AU14559, the OGC was found in the region between 455,968 and 481,635 bp.…”

Section: Resultsmentioning

confidence: 99%

High Genomic Identity between Clinical and Environmental Strains of Herbaspirillum frisingense Suggests Pre-Adaptation to Different Hosts and Intrinsic Resistance to Multiple Drugs

et al. 2021

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The genus Herbaspirillum is widely studied for its ability to associate with grasses and to perform biological nitrogen fixation. However, the bacteria of the Herbaspirillum genus have frequently been isolated from clinical samples. Understanding the genomic characteristics that allow these bacteria to switch environments and become able to colonize human hosts is essential for monitoring emerging pathogens and predicting outbreaks. In this work, we describe the sequencing, assembly, and annotation of the genome of H. frisingense AU14559 isolated from the sputum of patients with cystic fibrosis, and its comparison with the genomes of the uropathogenic strain VT-16–41 and the environmental strains GSF30, BH-1, IAC152, and SG826. The genes responsible for biological nitrogen fixation were absent from all strains except for GSF30. On the other hand, genes encoding virulence and host interaction factors were mostly shared with environmental strains. We also identified a large set of intrinsic antibiotic resistance genes that were shared across all strains. Unlike other strains, in addition to unique genomic islands, AU14559 has a mutation that renders the biosynthesis of rhamnose and its incorporation into the exopolysaccharide unfeasible. These data suggest that H. frisingense has characteristics that provide it with the metabolic diversity needed to infect and colonize human hosts.

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Section: Resultsmentioning

confidence: 99%