Structural and functional studies of the rat mitochondrial single strand DNA binding protein P16

Hoke, Glenn D.; Pavco, Pamela A.; Ledwith, Brian J.; Tuyle, G C Van

doi:10.1016/0003-9861(90)90094-f

Cited by 53 publications

(44 citation statements)

References 27 publications

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“…We distinguished several characteristic species. At concentrations of HsmtSSB below saturation of the template DNA, as predicted from the ssDNA binding site size for vertebrate mtSSB tetramers of 35 nt (13,24,25), the template maintains a compacted structure. Binding of SSB results in the appearance of the forms called "beaded" and "collapsed."…”

Section: Dna Polymerization Activity Of Pol ␥ Depends On the Molarmentioning

confidence: 99%

Mitochondrial Single-stranded DNA-binding Proteins Stimulate the Activity of DNA Polymerase γ by Organization of the Template DNA

Ciesielski

Bermek

Rosado-Ruiz

et al. 2015

Journal of Biological Chemistry

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Background: mtSSB stimulates the activity of Pol ␥. Results: Stimulation of Pol ␥ activity by SSB correlates with the organization of ssDNA templates in a species-independent manner. Conclusion: Organization of the template DNA by mtSSB is the major factor contributing to the stimulation of Pol ␥ activity. Significance: This study provides insight into the functional relationship of Pol ␥ and mtSSB and a general mechanism for it.

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Section: Dna Polymerization Activity Of Pol ␥ Depends On the Molarmentioning

confidence: 99%

Mitochondrial Single-stranded DNA-binding Proteins Stimulate the Activity of DNA Polymerase γ by Organization of the Template DNA

Ciesielski

Bermek

Rosado-Ruiz

et al. 2015

Journal of Biological Chemistry

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“…mtSSB binds to single-stranded DNA (ssDNA), coating the displaced ssDNA, which is the template for lagging DNA strand synthesis in mtDNA replication, and preventing it from renaturation . mtSSB also stimulates greatly the activity of pol ␥ from various species (Mignotte et al, 1988;Hoke et al 1990;Williams and Kaguni, 1994;Thöm-mes et al, 1995). Although the mtSSB gene was shown to be essential in yeast (van Dyck et al 1992), its role in mitochondrial biogenesis in animals has not been explored, despite the fact that it represents a candidate gene for inherited human mtDNA depletion.…”

Section: Introductionmentioning

confidence: 99%

Mitochondrial Single-stranded DNA-binding Protein Is Required for Mitochondrial DNA Replication and Development inDrosophila melanogaster

Maier

Farr

Poeck

et al. 2001

MBoC

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The discovery that several inherited human diseases are caused by mtDNA depletion has led to an increased interest in the replication and maintenance of mtDNA. We have isolated a new mutant in the lopo (low power) gene from Drosophila melanogaster affecting the mitochondrial single-stranded DNA-binding protein (mtSSB), which is one of the key components in mtDNA replication and maintenance. lopo 1 mutants die late in the third instar before completion of metamorphosis because of a failure in cell proliferation. Molecular, histochemical, and physiological experiments show a drastic decrease in mtDNA content that is coupled with the loss of respiration in these mutants. However, the number and morphology of mitochondria are not greatly affected. Immunocytochemical analysis shows that mtSSB is expressed in all tissues but is highly enriched in proliferating tissues and in the developing oocyte. lopo 1 is the first mtSSB mutant in higher eukaryotes, and its analysis demonstrates the essential function of this gene in development, providing an excellent model to study mitochondrial biogenesis in animals.

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“…A second mtDNA binding protein that is relatively well-characterized is mitochondrial single-stranded DNA binding protein (mtSSB) (12)(13)(14). mtSSB is a tetramer of ϳ16-kDa subunits with DNA binding characteristics and structure closely related to those of E. coli SSB (15)(16)(17). The helicase, twinkle, has also recently been shown to reside in the mtDNA nucleoid (18).…”

mentioning

confidence: 99%

Protein Components of Mitochondrial DNA Nucleoids in Higher Eukaryotes

Bogenhagen

Wang

Shen

et al. 2003

Molecular & Cellular Proteomics

135

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Structural and functional studies of the rat mitochondrial single strand DNA binding protein P16

Cited by 53 publications

References 27 publications

Mitochondrial Single-stranded DNA-binding Proteins Stimulate the Activity of DNA Polymerase γ by Organization of the Template DNA

Mitochondrial Single-stranded DNA-binding Proteins Stimulate the Activity of DNA Polymerase γ by Organization of the Template DNA

Mitochondrial Single-stranded DNA-binding Protein Is Required for Mitochondrial DNA Replication and Development inDrosophila melanogaster

Protein Components of Mitochondrial DNA Nucleoids in Higher Eukaryotes

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