Structural and functional measures of marine microbial communities: An experiment to assess implications for oil spill management

Morris, Liz; O’Brien, Allyson L.; Natera, Siria; Lutz, Adrian; Roessner, Ute; Long, Sara M.

doi:10.1016/j.marpolbul.2018.04.054

Cited by 16 publications

(9 citation statements)

References 30 publications

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“…In agreement with previous studies significant changes in the bacterial community were observed were observed after 48 h of exposition to crude oil [44,45,46]. During this time, considerable increases in the relative abundances of Alpha-proteobacteria and decreases in Cyanobacteria and Gamma-proteobacteria were observed (Figure 3b).…”

Section: Discussionsupporting

confidence: 92%

Changes in the Bacterioplankton Community Structure from Southern Gulf of Mexico During a Simulated Crude Oil Spill at Mesocosm Scale

et al. 2019

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The southern Gulf of Mexico (sGoM) is highly susceptible to receiving environmental impacts due to the recent increase in oil-related activities. In this study, we assessed the changes in the bacterioplankton community structure caused by a simulated oil spill at mesocosms scale. The 16S rRNA gene sequencing analysis indicated that the initial bacterial community was mainly represented by Gamma-proteobacteria, Alpha-proteobacteria, Flavobacteriia, and Cyanobacteria. The hydrocarbon degradation activity, measured as the number of culturable hydrocarbonoclastic bacteria (CHB) and by the copy number of the alkB gene, was relatively low at the beginning of the experiment. However, after four days, the hydrocarbonoclastic activity reached its maximum values and was accompanied by increases in the relative abundance of the well-known hydrocarbonoclastic Alteromonas. At the end of the experiment, the diversity was restored to similar values as those observed in the initial time, although the community structure and composition were clearly different, where Marivita, Pseudohongiella, and Oleibacter were detected to have differential abundances on days eight–14. These changes were related with total nitrogen (p value = 0.030 and r2 = 0.22) and polycyclic aromatic hydrocarbons (p value = 0.048 and r2 = 0.25), according to PERMANOVA. The results of this study contribute to the understanding of the potential response of the bacterioplankton from sGoM to crude oil spills.

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Section: Discussionsupporting

confidence: 92%

Changes in the Bacterioplankton Community Structure from Southern Gulf of Mexico During a Simulated Crude Oil Spill at Mesocosm Scale

et al. 2019

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“…), several studies have started to investigate the effects on marine biota, also including microbes, related to the presence of hydrocarbon contaminants [2], [10], [23], [24]. It is well known that a disturbance can modify microbial community composition [25] and, in turn, structural changes result in functional changes. To this regard, a reduction in microbial diversity was reported to affect general metabolic activity as well as the functional ability of the microbial community to degrade pollutants [26].…”

Section: Discussionmentioning

confidence: 99%

Ecological and Molecular Approach to the Assessment of Oil Pollution: A Comparative Study Between Two Coastal Marine (Mediterranean and Patagonian) Ecoregions

Zappalà

Caruso

Denaro

et al. 2020

WIT Transactions on Ecology and the Environment

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Microorganisms are considered as sentinels of environmental changes, but microbial response to the presence of contaminants, such as hydrocarbons, is still not fully understood. This study aimed at assessing the response of the microbial community to the presence of oil pollution, by comparison of two ecosystems, Milazzo Gulf (Sicily, Italy) and Caleta Cordova (Argentina), as representative of two different temperate Mediterranean and cold-temperate Patagonian ecoregions, respectively. Water and sediments were sampled at coastal stations characterized by different levels of hydrocarbon contamination and analyzed for their microbial enzymatic activity rates; the presence of hydrocarbondegrading bacteria was also determined. The study approach allowed to determine that microbial metabolism was significantly reduced at the polluted stations, suggesting the detrimental effects of contaminants on organic matter degradation process. The isolation of strains of hydrocarbon-degrading bacteria confirmed that the oil contamination favoured the growth of strains specifically adapted to metabolize hydrocarbons and actively involved in the remediation processes.

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“…The bacterial succession observed during this second phase of light crude oil degradation may be due to the preference of some micro‐organisms for the intermediates of hydrocarbon degradation as carbon source (Head et al ; Morris et al ). Correspondingly, the bacterial groups benefited in this phase were indirectly related to OTUs of the genus Alcanivorax by negative correlations.…”

Section: Discussionmentioning

confidence: 99%

Bacterial succession and co‐occurrence patterns of an enriched marine microbial community during light crude oil degradation in a batch reactor

Uribe-Flores

Cerqueda-García

Hernández-Nuñéz

et al. 2019

J of Applied Microbiology

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Aims The aim of this study was to investigate the dynamic changes in the bacterial structure and potential interactions of an acclimatized marine microbial community during a light crude oil degradation experiment. Methods and Results The bacterial community effectively removed 76·49% of total petroleum hydrocarbons after 30 days, as evidenced by GC‐FID and GC‐MS analyses. Short‐chain alkanes and specific aromatic compounds were completely degraded within the first 6 days. High‐throughput sequencing of 16S rRNA gene indicated that the starting bacterial community was mainly composed by Marinobacter and more than 30 non‐dominant genera. Bacterial succession was dependent on the hydrocarbon uptake with Alcanivorax becoming dominant during the highest degradation period. Sparse correlations for compositional data algorithm revealed one operational taxonomic unit (OTU) of Muricauda and an assembly of six OTUs of Alcanivorax dieselolei and Alcanivorax hongdengensis as critical keystone components for the consortium network maintenance and stability. Conclusions This work exhibits a stabilized marine bacterial consortium with the capability to efficiently degrade light crude oil in 6 days, under laboratory conditions. Successional and interaction patterns were observed in response to hydrocarbon consumption, highlighting potential interactions between Alcanivorax and keystone non‐dominant OTUs over time. Significance and Impact of the Study Our results contribute to the understanding of interactions and potential roles of specific members of hydrocarbonoclastic marine bacterial communities, which will be useful for further bioaugmentation studies concerning the associations between indigenous and introduced micro‐organisms.

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Structural and functional measures of marine microbial communities: An experiment to assess implications for oil spill management

Cited by 16 publications

References 30 publications

Changes in the Bacterioplankton Community Structure from Southern Gulf of Mexico During a Simulated Crude Oil Spill at Mesocosm Scale

Changes in the Bacterioplankton Community Structure from Southern Gulf of Mexico During a Simulated Crude Oil Spill at Mesocosm Scale

Ecological and Molecular Approach to the Assessment of Oil Pollution: A Comparative Study Between Two Coastal Marine (Mediterranean and Patagonian) Ecoregions

Bacterial succession and co‐occurrence patterns of an enriched marine microbial community during light crude oil degradation in a batch reactor

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