Structural and functional insights into colicin: a new paradigm in drug discovery

Calcuttawala, Fatema; Pal, Ankita; Nath, Papri; Kar, Riya; Hazra, Debraj; Pal, Rajat Kumar

doi:10.1007/s00203-021-02689-6

Cited by 11 publications

(13 citation statements)

References 54 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These proteins belong to the TonB-dependent copper receptor family (IPR010100) and perform the transport of nutrients across the periplasmic space [94–95]. Colicins in Escherichia coli are toxins that act in intraspecific competition [96–97], but their role in B. fragilis is unclear. Yoshizaki et al [90] compared the genomes of 638R, NCTC9343, and YCH46 and found 52 genes under positive selection.…”

Section: Resultsmentioning

confidence: 99%

Comprehensive Comparative Genomic revels:Bacteroides fragilisis a reservoir of antibiotic resistance genes in the gut microbiota

Evanovich

Mattos

Guerreiro

2022

Preprint

View full text Add to dashboard Cite

Bacteroides fragilis are commensal bacteria of the gut microbiota of mammals and may cause severe infection in a susceptible host. Treatment can be cumbersome if multidrug resistant strains are present in the affected tissue. The principal aim of this study was to provide new insights into the genomic properties of B. fragilis through different approaches in comparative genomics. Results revealed that the pan-genome is opened, and an intense exchange of genetic material reinforces this inference. The Don complex, responsible for extraintestinal adaptation, is present in all strains, suggesting a crucial role for B. fragilis adaptation. CRISPR-Cas system is at 76% of the samples, but it apparently has low accuracy against prophage. Multidrug resistance genes are in 80% of strains. Conjugative transposons and integrative and conjugative elements (ICE) are the main spreaders of genes for antimicrobial resistance. We also reported evidence for horizontal gene transfer (HGT) of antimicrobial resistance genes among the B. fragilis strains and Bacteriodales. At least 398 genes are under positive selection, including genes for antimicrobial resistance and transport of toxins and nutrients.

show abstract

Section: Resultsmentioning

confidence: 99%

Comprehensive Comparative Genomic revels:Bacteroides fragilisis a reservoir of antibiotic resistance genes in the gut microbiota

Evanovich

Mattos

Guerreiro

2022

Preprint

View full text Add to dashboard Cite

show abstract

“…The majority of bacteriocins studied so far are produced by Gram-positive bacteria and most often lactic acid bacteria, which are widely used in the food industry ( Arthur et al, 2014 ). However, numerous structurally diverse Gram-negative bacteriocins are also described ( Telhig et al, 2020 ; Calcuttawala et al, 2021 ). Bacteriocins find applications in a wide range of sectors including agri-food, and the human and veterinary medicine fields ( Stahl et al, 2004 ; Cutler et al, 2007 ; Soltani et al, 2021 ).…”

Section: Introductionmentioning

confidence: 99%

Impact of microcin J25 on the porcine microbiome in a continuous culture model

et al. 2022

View full text Add to dashboard Cite

The increased prevalence of Salmonella spp. resistance in swine spurs the search for alternatives to antibiotics. Microcin J25 (MccJ25), a bacteriocin produced by Escherichia coli, is a potent inhibitor of several pathogenic bacteria including Salmonella enterica. In this study, we aimed to evaluate in vitro the impact of MccJ25 on the composition and the metabolic activity of the swine colonic microbiota. The PolyFermS in vitro continuous fermentation model was used here with modified Macfarlane medium to simulate the porcine proximal colon. During 35 days of fermentation, a first-stage reactor containing immobilized swine fecal microbiota fed two second-stage control and test reactors operated in parallel and used to test the effects of MccJ25 on the composition and the metabolic activity of the microbiota. Reuterin, a broad-spectrum antimicrobial compound produced by Limosilactobacillus reuteri, a lactic acid bacterium naturally present in the gastro-intestinal tract of human and animals, and the antibiotic rifampicin were tested for comparison. Sequencing of 16S rRNA was performed using the Illumina MiSeq technology to evaluate microbial diversity, and liquid chromatography coupled to mass spectrometry (LC-MS) followed by multivariate analysis was used to assess the bacteriocin/antibiotic degradation products and to monitor changes in the swine colonic microbiota metabolome. The results show that MccJ25 or reuterin treatments only induce subtle changes of both the microbial diversity and the metabolome of the swine colon microbiota, while rifampicin induces significant modification in amino acid levels. Although these findings need being validated in vivo, this study affords a first proof of concept for considering MccJ25 as a possible alternative to antibiotics for veterinary and farming applications, taking into account its pathogen-selective and potent inhibitory activity.

show abstract

“…The third gene within the operon encoding a lysis protein, is regulated by the downstream SOS promoter. Thus, transcription of the lysis gene require the polymerase to transcribe through the entire operon which necessitate significant induction and prevent unnecessary lysis of the cell ( Calcuttawala et al., 2022 ; Yin et al., 2019 ).…”

Section: Introductionmentioning

confidence: 99%

Colicin E2 expression in Escherichia coli biofilms: Induction and regulation revisited

Bayramoglu-Güven

Ghazaryan

Toubiana

et al. 2022

Current Research in Microbial Sciences

View full text Add to dashboard Cite

Structural and functional insights into colicin: a new paradigm in drug discovery

Cited by 11 publications

References 54 publications

Comprehensive Comparative Genomic revels:Bacteroides fragilisis a reservoir of antibiotic resistance genes in the gut microbiota

Comprehensive Comparative Genomic revels:Bacteroides fragilisis a reservoir of antibiotic resistance genes in the gut microbiota

Impact of microcin J25 on the porcine microbiome in a continuous culture model

Colicin E2 expression in Escherichia coli biofilms: Induction and regulation revisited

Contact Info

Product

Resources

About