Structural and functional evolution of an auxin efflux carrier PIN1 and its functional characterization in common wheat

Singh, K. S.; Singh, Jit; Jindal, Suruchi; Sidhu, Gaganjot; Dhaliwal, Amandeep Kaur; Gill, Kulvinder S.

doi:10.1007/s10142-018-0625-9

Cited by 7 publications

(3 citation statements)

References 44 publications

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“…It was reported that virus-induced gene silencing of the TaPIN1 genes resulted in 26% reduction in plant height [32]. Multiple sequence alignment revealed that the TaPIN1 genes in the study by Singh et al [32] were similar to TaPIN1-7b in the phylogenetic analysis of our study (Fig. 1a).…”

Section: Discussionsupporting

confidence: 71%

Down-expression of TaPIN1s Increases the Tiller Number and Grain Yield in Wheat

Yao

Wang

et al. 2021

Preprint

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Background: Tiller number is a factor determining panicle number and grain yield in wheat (Triticum aestivum L.). Auxin plays an important role in the regulation of branch production. PIN-FORMED 1 (PIN1), an auxin efflux carrier, plays a role in the regulation of tiller number in rice (Oryza sativa); however, little is known on the roles of PIN1 in wheat. Results: Nine homologs of TaPIN1 genes were identified in wheat, of which TaPIN1-6 genes showed higher expression in the stem apex and young leaf in wheat, and the TaPIN1-6a protein was localized in the plasma membrane. The down-expression of TaPIN1s increased the tiller number in TaPIN1-RNA interference (TaPIN1-RNAi) transgenic wheat plants, indicating that auxin might mediate the axillary bud production. By contrast, the spikelet number, grain number per panicle, and the 1000-grain weight were decreased in the TaPIN1-RNAi transgenic wheat plants compared with those in the wild type. Conclusions: Phylogenetic analysis and expression patterns of nine TaPIN1 genes, and their protein structures and subcellular localization of TaPIN1-6a protein were analyzed. Down-regulated expression of TaPIN1 genes increased the tiller numbers of transgenic wheat lines. Our study suggests that TaPIN1s is required for the regulation of grain yield in wheat.

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Section: Discussionsupporting

confidence: 71%

Down-expression of TaPIN1s Increases the Tiller Number and Grain Yield in Wheat

Yao

Wang

et al. 2021

Preprint

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“…The M. polymorpha genome encodes five PIN genes (Bowman et al ., 2017). Only one, Mp PIN1 (Mp3g21660), also referred to as Mp PINZ in some previous publications, is orthologous with AtPIN1 and is predicted to be plasma membrane‐localised (Bennett et al ., 2014a; Singh et al ., 2019). The other four (Mp PIN2 / 3 / 4 / 5 ) each possess a short central hydrophilic loop, a feature associated with loss of plasma membrane localisation (Bennett et al ., 2014a).…”

Section: Resultsmentioning

confidence: 99%

PIN‐FORMED is required for shoot phototropism/gravitropism and facilitates meristem formation in Marchantia polymorpha

et al. 2023

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PIN-FORMED auxin efflux transporters, a subclass of which is plasma membrane-localised, mediate a variety of land-plant developmental processes via their polar localisation and subsequent directional auxin transport. We provide the first characterisation of PIN proteins in liverworts using Marchantia polymorpha as a model system. Marchantia polymorpha possesses a single PIN-FORMED gene, whose protein product is predicted to be plasma membranelocalised, MpPIN1.To characterise MpPIN1, we created loss-of-function alleles and produced complementation lines in both M. polymorpha and Arabidopsis. In M. polymorpha, gene expression and protein localisation were tracked using an MpPIN1 transgene encoding a translationally fused fluorescent protein.Overexpression of MpPIN1 can partially complement loss of an orthologous gene, PIN-FORMED1, in Arabidopsis. In M. polymorpha, MpPIN1 influences development in numerous ways throughout its life cycle. Most notably, MpPIN1 is required to establish gemmaling dorsiventral polarity and for orthotropic growth of gametangiophore stalks, where MpPIN1 is basally polarised.PIN activity is largely conserved within land plants, with PIN-mediated auxin flow providing a flexible mechanism to organise growth. Specifically, PIN is fundamentally linked to orthotropism and to the establishment of de novo meristems, the latter potentially involving the formation of both auxin biosynthesis maxima and auxin-signalling minima.

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“…It was reported that virus-induced gene silencing of the TaPIN1 genes resulted in 26% reduction in plant height [ 32 ]. Multiple sequence alignment revealed that the TaPIN1 genes in the study by Singh et al [ 32 ] were similar to TaPIN1-7b in the phylogenetic analysis of our study (Fig. 1 a).…”

Section: Discussionmentioning

confidence: 99%

Down-expression of TaPIN1s Increases the Tiller Number and Grain Yield in Wheat

Yao

et al. 2021

BMC Plant Biol

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Background Tiller number is a factor determining panicle number and grain yield in wheat (Triticum aestivum). Auxin plays an important role in the regulation of branch production. PIN-FORMED 1 (PIN1), an auxin efflux carrier, plays a role in the regulation of tiller number in rice (Oryza sativa); however, little is known on the roles of PIN1 in wheat. Results Nine homologs of TaPIN1 genes were identified in wheat, of which TaPIN1-6 genes showed higher expression in the stem apex and young leaf in wheat, and the TaPIN1-6a protein was localized in the plasma membrane. The down-expression of TaPIN1s increased the tiller number in TaPIN1-RNA interference (TaPIN1-RNAi) transgenic wheat plants, indicating that auxin might mediate the axillary bud production. By contrast, the spikelet number, grain number per panicle, and the 1000-grain weight were decreased in the TaPIN1-RNAi transgenic wheat plants compared with those in the wild type. In summary, a reduction of TaPIN1s expression increased the tiller number and grain yield per plant of wheat. Conclusions Phylogenetic analysis and protein structure of nine TaPIN1 proteins were analyzed, and subcellular localization of TaPIN1-6a was located in the plasma membrane. Knock-down expression of TaPIN1 genes increased the tiller number of transgenic wheat lines. Our study suggests that TaPIN1s is required for the regulation of grain yield in wheat.

show abstract

Structural and functional evolution of an auxin efflux carrier PIN1 and its functional characterization in common wheat

Cited by 7 publications

References 44 publications

Down-expression of TaPIN1s Increases the Tiller Number and Grain Yield in Wheat

Down-expression of TaPIN1s Increases the Tiller Number and Grain Yield in Wheat

PIN‐FORMED is required for shoot phototropism/gravitropism and facilitates meristem formation in Marchantia polymorpha

Down-expression of TaPIN1s Increases the Tiller Number and Grain Yield in Wheat

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