Structural and functional comparisons of the environmental microbiota of pond and tank environments at different locations for the commercial aquaculture of American shad

Abstract

The environmental microbiota plays a significant role in the growth and development of aquatic life. In recent years, American shad has become an important economic aquaculture species in China. However, information on the correlation between the growth of American shad and the aquaculture environment is limited. Through 16S rDNA‐based sequencing, the microbiota communities in ponds at different locations (Jiangyin and Yancheng in Jiangsu, China) were investigated. The results showed that the richness and diversity of the microbiota in the pond were greater than those in the tank at the same location. At the phylum level, Proteobacteria and Firmicutes were more abundant in the samples from Jiangyin than in those from Yancheng. In addition, the ratio of Firmicutes to Bacteroidetes was larger in the JYT samples than in the YCT samples, which implied that the American shad cultured in the Jiangyin environment tended to be fatter than those cultured in Yancheng. The body weight data also confirmed this finding. Moreover, the proportions of functional annotations in the samples from the Jiangyin and Yancheng environments were similar, but there were differences between the overall levels. Our results highlighted the correlations between the environmental microbiome and the growth tendency of American shad.

The potential of a combination of pungent spices as a novel supplement in gilthead seabream (Sparus aurata) diets to aid in the strategic use of fish oil in aquafeeds: a holistic perspective

“…No differences were found in the values of the F/B ratio, which is a well-documented factor whose changes have been used as a biomarker of intestinal dysbiosis in fishes ( 102 ). Our F/B values remain within the results reported for farmed fish, which vary widely depending on the rearing conditions and experimental diets utilized ( 103 , 104 ). On the other hand, the absence of differences in beta diversity (Weighted UniFrac) indicated that all samples were similar in terms of their phylogenetic features, and therefore, the addition of pungent spices to the diet did not pose a threat in terms of dysbiosis.…”

Connection between the Gut Microbiota of Largemouth Bass (Micropterus salmoides) and Microbiota of the aquaponics system Environment

“…Measurements of water temperature, pH, dissolved oxygen and conductivity were conducted in the eld utilizing the ProQuatro smart portable multiparameter water quality analyzer (YSI, Yellow Springs, OH, USA). In the light of a previously described method (Du et al 2022), the concentrations of ammonium nitrogen, nitrate nitrogen, nitrite nitrogen, total nitrogen and total phosphorus were determined. Phosphate levels were measured incompliance with the Chinese national environmental protection standard HJ 670-2013 "Water quality-Determination of orthophosphate and total phosphorus-Continuous ow analysis (CFA) and Ammonium molybdate spectrophotometry".…”

“…Phosphate levels were measured incompliance with the Chinese national environmental protection standard HJ 670-2013 "Water quality-Determination of orthophosphate and total phosphorus-Continuous ow analysis (CFA) and Ammonium molybdate spectrophotometry". The chemical oxygen demand was measured following the standard procedure outlined in ISO 8467:1993 (Du et al 2022).…”