Structural and functional characterization of a modified legionaminic acid involved in glycosylation of a bacterial lipopolysaccharide

McDonald, Nathan D.; DeMeester, Kristen E.; Lewis, Amanda L.; Grimes, Catherine L.; Boyd, E. Fidelma

doi:10.1074/jbc.ra118.004966

Cited by 4 publications

(9 citation statements)

References 62 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…6, panel b). While the former does not correspond to any identified NulO by this method, the latter can be tentatively assigned to the Leg5Ac7AcAlaQ compound observed in Vibrio vulnificus 39 . As mentioned above, the MS/MS spectra of the PseAc2 compounds from M. viscosa and Vibrio B9-25K2 show separate fragmentation patterns (see Fig.…”

Section: Sequence Analysis Of Nonulosonic Acid Biosynthetic Gene Clusmentioning

confidence: 68%

“…6 (panel b, top). The Leg5Ac7AcAla compound was not identified at the time of the experiment, and masses related to its quinoxaline were not investigated 39 . The presence of quinoxalines corresponding to PseAc2 (PseAc2Q: m/z = 451) was detected in both M. viscosa and Vibrio B9-25K2 (see Supplementary Information).…”

Section: Resultsmentioning

confidence: 99%

“…The two groups differ from each other in regions 3-8, indicating that they may have a different susbstrate specificity. 8eLeg5Am7Ac was detected in A. salmonicida LFI1238, while A. wodanis, whose cluster is closest to that of Vibrio vulnificus CMCP6, might produce Leg5Ac7AcAla (or a variant of it) 34,39 . The corresponding biosynthesis pathways seem to involve different NeuB susbtrates, which is consistent with the observations made here.…”

Section: Sequence Analysis Of Nonulosonic Acid Biosynthetic Gene Clusmentioning

confidence: 99%

“…This preliminary experimental characterization strengthens the hypotheses made after analysis of the NeuB homologs and the nab clusters concerning the type of sugar produced by each cluster. A similar approach has already been used successfully in several species and the method appears robust, although it is limited to detecting compounds similar (by either mass or gene cluster) to previously identified sugars 24,39,52 . Obviously, a more thorough experimental analysis is needed to fully characterize the compounds detected.…”

Section: Sequence Analysis Of Nonulosonic Acid Biosynthetic Gene Clusmentioning

confidence: 99%

“…As for A. salmonicida , Neu5Ac and Neu5Ac7(9)Ac were shown to be produced by M. viscosa 38 . The NulO content of A. wodanis has not been investigated so far, but its nab gene cluster appears similar to that of Vibrio vulnificus CMCP6, which produces the alanyl carrying Leg variant Leg5Ac7AcAla 39 . The diversity of nab clusters within Vibrionaceae has already been shown, with an emphasis on Vibrio vulnificus 28 .…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Sequence analysis of nonulosonic acid biosynthetic gene clusters in Vibrionaceae and Moritella viscosa

Halsør

Altermark

Ræder

2020

Sci Rep

View full text Add to dashboard Cite

investigated so far, but its nab gene cluster appears similar to that of Vibrio vulnificus CMCP6, which produces the alanyl carrying Leg variant Leg5Ac7AcAla 39. The diversity of nab clusters within Vibrionaceae has already been shown, with an emphasis on Vibrio vulnificus 28. The distribution of NulOs in Moritellaceae has not been studied. This study investigates the presence and composition of nab gene clusters in a set of strains from A. salmonicida (Vibrionaceae) and M. viscosa (Moritellaceae), as well as A. wodanis and a few other members of the Vibrionaceae family. The strains were previously isolated from different kinds of organisms such as fish, sponge and amphipod (for a summary of the strains used in this study, see Table 1 in the Materials and Methods section). They were screened for the presence of NeuB sequences, which were then used to locate nab clusters in their genomes as well as investigate the sequence determinants of NeuB substrate specificity. Each cluster was analyzed in terms of gene content and organization, using sequence homology as a basis for functional annotation. From this, hypotheses pertaining to the nature of the NulO(s) produced by each strain could be made, and a set of candidates for experimental analysis was obtained. The NulO content of a few strains was analyzed using mass spectrometry in order to obtain preliminary experimental results. Results and discussion comparison of neuB sequences. Sequence alignment and phylogeny. NeuB protein sequences from A. salmonicida LFI1238, M. viscosa 06/09/139 and A. wodanis were used as queries for conducting a sequence similarity search within the set of target genomes (see Table 1). Out of the 15 targets, 10 had at least one sequence identical to one of the queries, and 3 had a similar sequence. As expected, no NeuB coding sequences were found in either P. phosphoreum or V. anguillarum. A fingerprint of the alignment of unique NeuB sequences and NeuB phylogeny are presented in Fig. 2 (the full alignment is included as Supplementary Information, in Figure S1). Six of the eight targeted M. viscosa strains have two NeuB coding sequences identical to the ones from the 06/09/139 strain. The two remaining strains contain each one sequence, identical to either the Neu-pathway homolog (strain F57) or the Pse-pathway homolog (strain NVI-5482). This is consistent with the evolutionary relations between M. viscosa genomes according to which those two strains form their own clade 40. It is also interesting to note that the strains with identical sequences were isolated from either salmond or rainbow trout, while the NVI-5482 and F57 were obtained from cod and lumpfish, respectively. For the Aliivibrio strains, two strains (A. salmonicida R8-68 and R8-70, isolated from the amphipod Eurythenes gryllus) have sequences

show abstract

Section: Sequence Analysis Of Nonulosonic Acid Biosynthetic Gene Clusmentioning

confidence: 68%

Section: Resultsmentioning

confidence: 99%

Section: Sequence Analysis Of Nonulosonic Acid Biosynthetic Gene Clusmentioning

confidence: 99%

Section: Sequence Analysis Of Nonulosonic Acid Biosynthetic Gene Clusmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Sequence analysis of nonulosonic acid biosynthetic gene clusters in Vibrionaceae and Moritella viscosa

Halsør

Altermark

Ræder

2020

Sci Rep

View full text Add to dashboard Cite

show abstract

Structural and Biosynthetic Diversity of Nonulosonic Acids (NulOs) That Decorate Surface Structures in Bacteria

McDonald

Boyd

2021

Trends in Microbiology

View full text Add to dashboard Cite

Virulent properties and genomic diversity of Vibrio vulnificus isolated from environment, human, diseased fish

Naknaen,

Surachat,

Manit

et al. 2024

Microbiol Spectr

View full text Add to dashboard Cite

The incidence of Vibrio vulnificus infections, with high mortality rates in humans and aquatic animals, has escalated, highlighting a significant public health challenge. Currently, reliable markers to identify strains with high virulence potential are lacking, and the understanding of evolutionary drivers behind the emergence of pathogenic strains is limited. In this study, we analyzed the distribution of virulent genotypes and phenotypes to discern the infectious potential of V. vulnificus strains isolated from three distinct sources. Most isolates, traditionally classified as biotype 1, possessed the virulence-correlated gene-C type. Environmental isolates predominantly exhibited YJ-like alleles, while clinical and diseased fish isolates were significantly associated with the nanA gene and pathogenicity region XII. Hemolytic activity was primarily observed in the culture supernatants of clinical and diseased fish isolates. Genetic relationships, as determined by multiple-locus variable-number tandem repeat analysis, suggested that strains originating from the same source tended to cluster together. However, multilocus sequence typing revealed considerable genetic diversity across clusters and sources. A phylogenetic analysis using single nucleotide polymorphisms of diseased fish strains alongside publicly available genomes demonstrated a high degree of evolutionary relatedness within and across different isolation sources. Notably, our findings reveal no direct correlation between phylogenetic patterns, isolation sources, and virulence capabilities. This underscores the necessity for proactive risk management strategies to address pathogenic V. vulnificus strains emerging from environmental reservoirs. IMPORTANCE As the global incidence of Vibrio vulnificus infections rises, impacting human health and marine aquacultures, understanding the pathogenicity of environmental strains remains critical yet underexplored. This study addresses this gap by evaluating the virulence potential and genetic relatedness of V. vulnificus strains, focusing on environmental origins. We conduct an extensive genotypic analysis and phenotypic assessment, including virulence testing in a wax moth model. Our findings aim to uncover genetic and evolutionary factors that drive pathogenic strain emergence in the environment. This research advances our ability to identify reliable virulence markers and understand the distribution of pathogenic strains, offering significant insights for public health and environmental risk management.

show abstract

Structural and functional characterization of a modified legionaminic acid involved in glycosylation of a bacterial lipopolysaccharide

Cited by 4 publications

References 62 publications

Sequence analysis of nonulosonic acid biosynthetic gene clusters in Vibrionaceae and Moritella viscosa

Sequence analysis of nonulosonic acid biosynthetic gene clusters in Vibrionaceae and Moritella viscosa

Structural and Biosynthetic Diversity of Nonulosonic Acids (NulOs) That Decorate Surface Structures in Bacteria

Virulent properties and genomic diversity of Vibrio vulnificus isolated from environment, human, diseased fish

Contact Info

Product

Resources

About