Structural and functional characterization of annexin 1 from Medicago truncatula

Kodavali, Praveen Kumar; Skowronek, Krzysztof; Koszela-Piotrowska, Izabela; Strzelecka‐Kiliszek, Agnieszka; Pawłowski, Krzysztof; Pikula, Sławomir

doi:10.1016/j.plaphy.2013.08.010

Cited by 15 publications

(13 citation statements)

References 48 publications

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“…When transporting K + , three different single channel conductances were observed; 16pS, 135 pS and 329 pS depending on the amount of annexin present. This is similar to channel behaviour shown by animal annexins in bilayers, where amount of protein is linked to level of oligomerization, association with the bilayer and channel characteristics such as conductance and voltage dependence [ 13 , 29 , 38 , 42 , 43 ]. Testing for Ca 2+ transport by MtANN1 is now feasible.…”

Section: Ca 2+ Transport By Plant Annexinssupporting

confidence: 62%

“…Single channel behaviour (discrete, step changes in current translocated across the bilayer) has rarely been observed but a single channel conductance of 17 pS was recorded in MDA-containing bilayers [ 38 ]. Recombinant Medicago truncatula Annexin 1 (MtANN1) has recently been shown to support single channel activity in planar lipid bilayers [ 42 ]. When transporting K + , three different single channel conductances were observed; 16pS, 135 pS and 329 pS depending on the amount of annexin present.…”

Section: Ca 2+ Transport By Plant Annexinsmentioning

confidence: 99%

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Annexin-Mediated Calcium Signalling in Plants

Davies

2014

Plants

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Calcium-permeable channels underpin elevations of free calcium that encode specific signals in stress adaptation, development and immunity. Identifying the genes encoding these channels remains a central goal of plant signalling research. Evidence now suggests that members of the plant annexin family function as unconventional calcium-permeable channels, with roles in development and stress signalling. Arabidopsis annexin 1 mediates a plasma membrane calcium-permeable conductance in roots that is activated by reactive oxygen species. Recombinant annexin 1 forms a very similar conductance in planar lipid bilayers, indicating that this protein could facilitate the in vivo conductance directly. The annexin 1 mutant is impaired in salinity-induced calcium signalling. Protein–protein interactions, post-translational modification and dynamic association with membranes could all influence annexin-mediated calcium signalling and are reviewed here. The prospect of annexins playing roles in calcium signalling events in symbiosis and immunity are considered.

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Section: Ca 2+ Transport By Plant Annexinssupporting

confidence: 62%

Section: Ca 2+ Transport By Plant Annexinsmentioning

confidence: 99%

Annexin-Mediated Calcium Signalling in Plants

Davies

2014

Plants

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“…Channel members from the families proposed to encode weakly selective plasma membrane cation channels have been suggested through in planta genetic analyses to be involved in transport of different cations, including that of Ca 2+ (Ali et al ., ; Laohavisit et al ., ; Wang et al ., ; Kong et al ., ), but their electrophysiological properties have not been extensively characterized. Medicago annexin 1 ( Medtr8g038210 ), coding for one of the six annexins present in root hairs (de Carvalho‐Niebel et al ., ; Figure ), has been shown to conduct cations across a planar bilayer membrane (Kodavali et al ., ). However, lack of information on regulation of Medicago annexin 1 by voltage prevents comparison with the cationic conductances characterized in the present study.…”

Section: Discussionmentioning

confidence: 97%

A repertoire of cationic and anionic conductances at the plasma membrane of Medicago truncatula root hairs

et al. 2019

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Root hairs, as lateral extensions of epidermal cells, provide large absorptive surfaces to the root and are major actors in plant hydromineral nutrition. In contact with the soil they also constitute a site of interactions between the plant and rhizospheric microorganisms. In legumes, initiation of symbiotic interactions with N 2 -fixing rhizobia is often triggered at the root hair cell membrane in response to nodulation factors secreted by rhizobia, and involves early signaling events with changes in H + , Ca 2+ , K + and Cl À fluxes inducing transient depolarization of the cell membrane. Here, we aimed to build a functional repertoire of the major root hair conductances to cations and anions in the sequenced legume model Medicago truncatula. Five root hair conductances were characterized through patch-clamp experiments on enzymatically recovered root hair protoplasts. These conductances displayed varying properties of voltage dependence, kinetics and ion selectivity. They consisted of hyperpolarization-and depolarization-activated conductances for K + , cations or Cl À . Among these, one weakly outwardly rectifying cationic conductance and one hyperpolarization-activated slowly inactivating anionic conductance were not known as active in root hairs. All five conductances were detected in apical regions of young growing root hairs using membrane spheroplasts obtained by laser-assisted cell-wall microdissection. Combined with recent root hair transcriptomes of M. truncatula, this functional repertoire of conductances is expected to help the identification of candidate genes for reverse genetics studies to investigate the possible role of each conductance in root hair growth and interaction with the biotic and abiotic environment.

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“…We found an upregulation of MLO 14 like protein in root, a recent study of MLO mutant revealed its involvement in polar auxin transport which is one of the components jointly responsible for an aberrant root phenotype 65 . A channeling protein, Annexin (ANNAT7) is reported to be involved in lateral root development by modulating the Ca 2+ ion gradient 66, 67 . A defensin-like protein, DEFL203, was expressed at its maximum level in the P 20 treatment.…”

Section: Discussionmentioning

confidence: 99%

Comprehensive study of excess phosphate response reveals ethylene mediated signaling that negatively regulates plant growth and development

Shukla

Rinehart

Sahi

2017

Sci Rep

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Excess Phosphorus (P) in agriculture is causing serious environmental problems like eutrophication of lakes and rivers. Unlike the enormous information available for phosphate starvation response (P0), very few information is available for the effect of excess phosphate Pi on plants. Characterization of Excess Phosphate Response (EPiR) is essential for designing strategies to increase phosphate accumulation and tolerance. We show a significant modulation in the root developmental plasticity under the increasing supply of excess Pi. An excess supply of 20 mM Pi (P20) produces a shallow root system architecture (RSA), reduces primary root growth, root apical meristem size, and meristematic activity in Arabidopsis. The inhibition of primary root growth and development is indeterminate in nature and caused by the decrease in number of meristematic cortical cells due to EPiR. Significant changes occurred in metal nutrients level due to excess Pi supply. A comparative microarray investigation of the EPiR response reveals a modulation in ethylene biosynthesis and signaling, metal ions deficiency response, and root development related genes. We used ethylene-insensitive or sensitive mutants to provide more evidence for ethylene-mediated signaling. A new role of EPiR in regulating the developmental responses of plants mediated by ethylene has been demonstrated.

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Structural and functional characterization of annexin 1 from Medicago truncatula

Cited by 15 publications

References 48 publications

Annexin-Mediated Calcium Signalling in Plants

Annexin-Mediated Calcium Signalling in Plants

A repertoire of cationic and anionic conductances at the plasma membrane of Medicago truncatula root hairs

Comprehensive study of excess phosphate response reveals ethylene mediated signaling that negatively regulates plant growth and development

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