Structural and functional characterization of neuropeptides involved in the control of male mating behavior of Lymnaea stagnalis

Li, K.W.; Smit, A.B.; Geraerts, W.P.M.

doi:10.1016/0196-9781(92)90166-z

Cited by 57 publications

(39 citation statements)

References 7 publications

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“…In Lymnaea stagnalis, the penial complex is innervated by multiple peptides (Li et al, 1992;van Kesteren et al, 1995a;de Lange et al, 1998). Neurons innervating the penial complex originate in the right cerebral, pleural, pedal, and parietal ganglia (de Boer et al, 1996;de Lange et al, 1998).…”

Section: Innervation Of the Reproductive Tractmentioning

confidence: 98%

Gonadotropin-releasing hormone neuronal system of the freshwater snailsHelisoma trivolvis andLymnaea stagnalis: Possible involvement in reproduction

1999

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Section: Innervation Of the Reproductive Tractmentioning

confidence: 98%

Gonadotropin-releasing hormone neuronal system of the freshwater snailsHelisoma trivolvis andLymnaea stagnalis: Possible involvement in reproduction

1999

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“…A cDNA clone has been characterized that encodes a precursor protein expressed in these cells . The predicted precursor contains multiple copies of the sequence Ala-Pro-Gly-Trp-Gly (which is posttranslationally processed to generate Ala-Pro-Gly-Trp-amide [APGWamide]), four connecting peptide sequences, and a Cterminal anterior lobe peptide (CALP; Li et al, 1992). Neurons of the right anterior lobe constitute the major site of expression of the APGWamide gene in Lymnaea (Croll et al, 1991b;Croll and Van Minnen, 1992), suggesting that peptides derived from the precursor are secreted at or near the penial complex and are involved in the control of aspects of male mating behavior.…”

mentioning

confidence: 99%

Molecular cloning of a cDNA encoding the neuropeptides APGWamide and cerebral peptide 1: Localization of APGWamide-like immunoreactivity in the central nervous system and male reproductive organs ofAplysia

Fan

Croll

et al. 1997

J. Comp. Neurol.

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While much is known about the neural and endocrine mechanisms that control egg laying in the gastropod mollusk Aplysia, relatively little is known about the regulation of male reproductive activity in this simultaneous hermaphrodite. In the present study, we have cloned and sequenced a cDNA that encodes a precursor protein, the predicted posttranslational processing of which presumably generates nine copies of the neuropeptide Ala-Pro-Gly-Trp-NH2 (APGWamide), five connecting peptide sequences, and a C-terminal peptide. The sequence of one connecting peptide is identical to the previously characterized cerebral peptide 1. Northern blot analysis identified two major APGWamide mRNA transcripts (approximately 1.3 kb, approximately 2.4 kb), which were present in central nervous system ganglia, but were most abundant in the right cerebral and right pedal ganglia. Immunohistochemical studies using sexually mature Aplysia demonstrated that the vast majority of APGWamide-like immunoreactivity was localized in 30-40 neurons along the anterior and medial margins of the right cerebral ganglion and in a cluster of 15-20 neurons in the right pedal ganglion. A total of only about ten immunoreactive neurons were located in other ganglia. Immunohistochemistry also demonstrated that APGWamide was present in the reproductive organs that participate in the storage or transport of sperm, including the small hermaphroditic duct (site of sperm storage before mating), the white hemiduct (also known as the copulatory duct), and penial complex. As a group, these data suggest that APGWamide may play a role in regulating male reproductive function in Aplysia, as it does in other gastropods.

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“…Besides APGWamide, a whole slew of other neuropeptides seems to be involved in the movement of the male copulatory apparatus (e.g. De Lange et al, 1998;Croll et al, 1991;Li et al, 1992;Van Golen et al, 1995a, Van Golen et al, 1995bSmit et al, 2003). Based on our findings we suggest that many of these will be involved in the fine-tuned movements of the male copulatory apparatus after eversion, i.e.…”

Section: Discussionmentioning

confidence: 85%

“…the preputium muscle bands (Croll et al, 1991;Li et al, 1992). In vivo, the injection of this neuropeptide into the blood indeed induces eversion of the preputium (De Boer et al, 1997b).…”

Section: Discussionmentioning

confidence: 99%

“…In the muscle bands, myomodulins, APGWamide, DEILSR and LIP have been found (De Lange et al, 1998). In vitro, APGWamide, Lymnaea Neuropeptide Y (LyNPY), GDPFLRF and SDPFLRF have a relaxing effect on the muscle bands (Croll et al, 1991;Li et al, 1992;Van Golen et al, 1995a) (Van Golen et al, 1995b;Smit et al, 2003), as do two of the myomodulins [SLSMLRL and GLQMLRL (Van Golen et al, 1996)]. The other two myomodulins increase the contraction's amplitude [SMSMLRL and PMSMLRL (Van Golen et al, 1996)].…”

Section: Introductionmentioning

confidence: 99%

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The distinction between retractor and protractor muscles of the freshwater snail's male organ has no physiological basis

Boer¹,

Jansen²,

Maat

et al. 2010

Journal of Experimental Biology

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SUMMARYMany animals are equipped with organs that can be everted, a notable example being male copulatory organs. The ability to protrude or evert an organ generally requires protractor and retractor muscles. Male copulatory behaviour of the pond snail Lymnaea stagnalis (L.) involves eversion (protraction) and retraction of the relatively large penis-carrying organ. For this preputium, protractor and retractor muscle bands have been defined, which implies eversion and retraction through the activity of these muscle bands. However, no physiological data are available that confirm that the terms protractor and retractor are appropriate. To test whether eversion and retraction are possible without protractor and/or retractor muscle bands, lesion experiments were performed. The results show that with either one or several muscle bands lesioned, snails were still capable of everting their preputium and using it for copulation. However, the majority of animals that had six or more muscle bands lesioned were unable to retract its preputium. Hence, retractor muscle bands serve their designated function whereas protractor muscle bands do not. We therefore suggest that a different terminology is used in which all muscle bands are retractors and, based on their location, are either called distal or proximal retractors. The findings furthermore indicate that the preputium muscle bands are normally contracted, possibly in a catch state, retaining the organ inside without high-energy expenditure.

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Structural and functional characterization of neuropeptides involved in the control of male mating behavior of Lymnaea stagnalis

Cited by 57 publications

References 7 publications

Gonadotropin-releasing hormone neuronal system of the freshwater snailsHelisoma trivolvis andLymnaea stagnalis: Possible involvement in reproduction

Gonadotropin-releasing hormone neuronal system of the freshwater snailsHelisoma trivolvis andLymnaea stagnalis: Possible involvement in reproduction

Molecular cloning of a cDNA encoding the neuropeptides APGWamide and cerebral peptide 1: Localization of APGWamide-like immunoreactivity in the central nervous system and male reproductive organs ofAplysia

The distinction between retractor and protractor muscles of the freshwater snail's male organ has no physiological basis

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