Structural and functional characterization of the deep-sea thermophilic bacteriophage GVE2 tailspike protein

Zhang, Likui; Yan, Yehai; Gan, Qi; She, Zhun; Zhu, Keli; Wang, Jinhui; Gao, Zhonghui; Dong, Yuhui; Gong, Yong

doi:10.1016/j.ijbiomac.2020.09.053

Cited by 4 publications

(4 citation statements)

References 48 publications

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“…The CTD of SspE contains a DUF1524 domain that belongs to the His-Asn-His (HNH, the last histidine of which can be replaced by an asparagine, as observed in SspE) nuclease superfamily and indeed exerts DNA nickase activity in vitro 8 . In contrast to the typical HNH motif in Cas9 14 , GVE2 15 , ScoMcrA 16 , ColE7 17 , or BrxU 18 that consists of two antiparallel β-sheets and an α-helix, the HNH motif in the CTD of SspE from S. yokosukanensis DSM 40224 was composed of two antiparallel β-sheets and a loop, suggesting comparatively higher flexibility (Fig. 1b ).…”

Section: Resultsmentioning

confidence: 95%

Nicking mechanism underlying the DNA phosphorothioate-sensing antiphage defense by SspE

et al. 2022

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DNA phosphorothioate (PT) modification, with a nonbridging phosphate oxygen substituted by sulfur, represents a widespread epigenetic marker in prokaryotes and provides protection against genetic parasites. In the PT-based defense system Ssp, SspABCD confers a single-stranded PT modification of host DNA in the 5′-CPSCA-3′ motif and SspE impedes phage propagation. SspE relies on PT modification in host DNA to exert antiphage activity. Here, structural and biochemical analyses reveal that SspE is preferentially recruited to PT sites mediated by the joint action of its N-terminal domain (NTD) hydrophobic cavity and C-terminal domain (CTD) DNA binding region. PT recognition enlarges the GTP-binding pocket, thereby increasing GTP hydrolysis activity, which subsequently triggers a conformational switch of SspE from a closed to an open state. The closed-to-open transition promotes the dissociation of SspE from self PT-DNA and turns on the DNA nicking nuclease activity of CTD, enabling SspE to accomplish self-nonself discrimination and limit phage predation, even when only a small fraction of modifiable consensus sequences is PT-protected in a bacterial genome.

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Section: Resultsmentioning

confidence: 95%

Nicking mechanism underlying the DNA phosphorothioate-sensing antiphage defense by SspE

et al. 2022

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Section: Barophilic Virusesmentioning

confidence: 99%

“…1137 [117]. Analysis of the characterized deep-sea virion GVE2 [118] has shown that it encodes a thermostable His-Asn-His (HNH) endonuclease [119,120] and that its tail spike protein has unique traits along with the ability to slow down the growth of its host cell Geobacillus [10]. Asn-His (HNH) endonuclease [119,120] and that its tail spike protein has unique traits along with the ability to slow down the growth of its host cell Geobacillus [10].…”

Section: Barophilic Virusesmentioning

confidence: 99%

“…Historically, the study of viruses has relied on the cultivation of a host cell and the replication of the cell’s requirements within a laboratory setting. This also requires the extraction of viable virions from the environment or samples collected from the environment [ 7 , 8 , 9 , 10 ]. This presents its own set of challenges, as these viruses must be infectious for there to be enough viable sample to study.…”

Section: Introductionmentioning

confidence: 99%

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The Expanding Diversity of Viruses from Extreme Environments

Manuel,

Snyder

2024

IJMS

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Viruses are nonliving biological entities whose host range encompasses all known forms of life. They are deceptively simple in description (a protein shell surrounding genetic material with an occasional lipid envelope) and yet can infect all known forms of life. Recently, due to technological advancements, viruses from more extreme environments can be studied through both culture-dependent and independent means. Viruses with thermophilic, halophilic, psychrophilic, and barophilic properties are highlighted in this paper with an emphasis on the properties that allow them to exist in said environments. Unfortunately, much of this field is extremely novel and thus, not much is yet known about these viruses or the microbes they infect when compared to non-extremophilic host–virus systems. With this review, we hope to shed some light on these relatively new studies and highlight their intrinsic value.

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Structural and functional basis of bacteriophage K64-ORF41 depolymerase for capsular polysaccharide degradation of Klebsiella pneumoniae K64

Huang,

Zhang,

Tao

et al. 2024

International Journal of Biological Macromolecules

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Structural and functional characterization of the deep-sea thermophilic bacteriophage GVE2 tailspike protein

Cited by 4 publications

References 48 publications

Nicking mechanism underlying the DNA phosphorothioate-sensing antiphage defense by SspE

Nicking mechanism underlying the DNA phosphorothioate-sensing antiphage defense by SspE

The Expanding Diversity of Viruses from Extreme Environments

Structural and functional basis of bacteriophage K64-ORF41 depolymerase for capsular polysaccharide degradation of Klebsiella pneumoniae K64

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