Structural and functional characterization of two-domain laccase from Streptomyces viridochromogenes

Трубицина, Л.И.; Tishchenko, Svetlana; Габдулхаков, А.Г.; Lisov, A. V.; Zakharova, Marina; Leontievsky, A. A.

doi:10.1016/j.biochi.2015.03.005

Cited by 47 publications

(30 citation statements)

References 58 publications

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“…S2 ) established that the enzyme was catalytically active over a range of pH, oxidizing ABTS at pH 5 ( k cat / K m 14 ± 0.5 mM −1 s −1 ) and syringol (2,6-dimethoxyphenol) at pH 8 ( k cat / K m 0.17 ± 0.04 mM −1 s −1 ). These spectroscopic and kinetic properties are comparable to those reported for sLac from Streptomyces veridochromogenes (sLac SvSL ) 27 .…”

Section: Resultssupporting

confidence: 82%

Enhanced delignification of steam-pretreated poplar by a bacterial laccase

Singh

Regner

et al. 2017

Sci Rep

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The recalcitrance of woody biomass, particularly its lignin component, hinders its sustainable transformation to fuels and biomaterials. Although the recent discovery of several bacterial ligninases promises the development of novel biocatalysts, these enzymes have largely been characterized using model substrates: direct evidence for their action on biomass is lacking. Herein, we report the delignification of woody biomass by a small laccase (sLac) from Amycolatopsis sp. 75iv3. Incubation of steam-pretreated poplar (SPP) with sLac enhanced the release of acid-precipitable polymeric lignin (APPL) by ~6-fold, and reduced the amount of acid-soluble lignin by ~15%. NMR spectrometry revealed that the APPL was significantly syringyl-enriched relative to the original material (~16:1 vs. ~3:1), and that sLac preferentially oxidized syringyl units and altered interunit linkage distributions. sLac’s substrate preference among monoaryls was also consistent with this observation. In addition, sLac treatment reduced the molar mass of the APPL by over 50%, as determined by gel-permeation chromatography coupled with multi-angle light scattering. Finally, sLac acted synergistically with a commercial cellulase cocktail to increase glucose production from SPP ~8%. Overall, this study establishes the lignolytic activity of sLac on woody biomass and highlights the biocatalytic potential of bacterial enzymes.

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Section: Resultssupporting

confidence: 82%

Enhanced delignification of steam-pretreated poplar by a bacterial laccase

Singh

Regner

et al. 2017

Sci Rep

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“…This enzyme shows high resistance to alkaline conditions and to high concentrations of sodium chloride [ 6 ], suggesting a robustness, which may be of commercial significance. Moreover, the enzyme contains two cupredoxine domains in its structure, as has been reported for other bacterial laccases [ 22 ]. The unusual properties of this newly isolated laccase make it worthy of further study, particularly in terms of elucidating the biological function of this laccase in the producer strain with the aim of assessing its potential for future commercial exploitation.…”

Section: Introductionmentioning

confidence: 92%

Laccase SilA from Streptomyces ipomoeae CECT 3341, a key enzyme for the degradation of lignin from agricultural residues?

et al. 2017

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The role of laccase SilA produced by Streptomyces ipomoeae CECT 3341 in lignocellulose degradation was investigated. A comparison of the properties and activities of a laccase-negative mutant strain (SilA−) with that of the wild-type was studied in terms of their ability to degrade lignin from grass lignocellulose. The yields of solubilized lignin (acid precipitable polymeric lignin, APPL) obtained from wheat straw by both strains in Solid State Fermentation (SSF) conditions demonstrated the importance of SilA laccase in lignin degradation with the wild-type showing 5-fold more APPL produced compared with the mutant strain (SilA−). Analytical pyrolysis and FT-IR (Fourier Transform Infrared Spectroscopy) confirmed that the APPL obtained from the substrate fermented by wild-type strain was dominated by lignin derived methoxyphenols whereas those from SilA− and control APPLs were composed mainly of polysaccharides. This is the first report highlighting the role of this laccase in lignin degradation.

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“…Laccases are recognized biocatalysts, which have the capacity to oxidize different phenolic compounds by using oxygen as an electron acceptor (Majumdar et al 2014). Recently, laccases characterized by the presence of two cupredoxine domains were identified in Streptomycetes (Molina-guijarro et al 2009;Trubitsina et al 2015). Their ability to withstand harsh conditions like high temperature and a range of pH draws the attention for biotechnological purposes.…”

Section: Laccasesmentioning

confidence: 99%

Microbial delignification and hydrolysis of lignocellulosic biomass to enhance biofuel production: an overview and future prospect

2019

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Background: The depletion of fossil fuel and its huge environmental problem are currently a concern for a scientific community in the area of energy engineering. This opened research opportunities for searching alternate renewable energy sources especially biofuel production from lignocellulose biomass resources. The main objective of this paper is to review the delignification and hydrolysis capabilities of microorganisms (bacteria and fungi). Results: Currently, different types of lignocellulose biomass pretreatment technologies are available. All of the technologies are either in lab scale or in pilot scale. Among the pretreatment technologies, biological pretreatments attract many attentions because of their eco-friendly advantages, performed at a mild temperature and do not produce inhibitory compounds during the pretreatment process. Industrial-scale biofuel production using biological delignification and hydrolysis process is still at lab scale, and intensive research works are required. The cost of biofuel production from lignocellulose biomass is currently expensive. Conclusion: Searching for the best microbial strains having efficient lignin-degrading and polysaccharide-hydrolyzing capabilities is vital to realize industrial-scale biofuel production from lignocellulose biomass. Process optimization along with genetic engineering of microorganisms is seen as a potential for biofuel production from lignocellulose biomass.

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Structural and functional characterization of two-domain laccase from Streptomyces viridochromogenes

Cited by 47 publications

References 58 publications

Enhanced delignification of steam-pretreated poplar by a bacterial laccase

Enhanced delignification of steam-pretreated poplar by a bacterial laccase

Laccase SilA from Streptomyces ipomoeae CECT 3341, a key enzyme for the degradation of lignin from agricultural residues?

Microbial delignification and hydrolysis of lignocellulosic biomass to enhance biofuel production: an overview and future prospect

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