Structural and Functional Characterization of β−lytic Protease from Lysobacter capsici VKM B−2533T

Afoshin, Alexey S.; Tishchenko, Svetlana; Габдулхаков, А.Г.; Kudryakova, Irina V.; Galemina, Inna; Zelenov, Dmitry; Leontyevskaya, Elena A.; Saharova, Sofia; Vasilyeva, Natalya Leontyevskaya

doi:10.3390/ijms232416100

Cited by 3 publications

(3 citation statements)

References 39 publications

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“…Lysobacter, Thiobacillus, Antarcticibacterium, Sphingomonas, Salinimicrobium, Pontibacter, Gemmatimonas, Ellin6067, RB41, Gillisia, Zeaxanthinibacter, MND1, Erythrobacter, and Intrasporangium were dominant in sea rice soils, with the sum of 34.01%. The potential proteolytic activity of the top 50 abundant genera of the bacterial community was shown (Table S2), among which Lysobacter, Pontibacter, Arthrobacter, Pseudomonas, Bacillus, Streptomyces, and Planococcus has been previously reported with the proteinase-producing strains [23][24][25][26][27][28][29][30][31], Arthrobacter, Pseudomonas, Bacillus, Streptomyces, Planococcus, and Nocardioides were isolated as proteinaseproducing bacteria by the subsequent culture-dependent methods in this study. The genera in red font were isolated as proteinase-producing bacteria by the subsequent culture-dependent methods or have been previously reported with the proteinase-producing strains.…”

Section: Alpha Diversity and Taxonomy Composition Analysis Of Bacteri...mentioning

confidence: 77%

Impact of Sea Rice Planting on Enzymatic Activity and Microbial Community of Coastal Soils: Focus on Proteinase

et al. 2023

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Soil proteinase and proteinase-producing microbial community are closely associated with soil fertility and soil health. Sea rice has been planted in the coastal beach of Jiaozhou Bay, China, in an effort to transform saline-alkali soil into arable land. However, the knowledge regarding the bacterial degradation of organic nitrogen in sea rice soils is limited. This study aims to investigate the physicochemical characteristics and enzymatic activities of the sea rice soils, as well as the microbial communities by both the Illumina sequencing-based culture-independent technology and culture-dependent methods. Sea rice soils exhibited a lower salinity and higher organic matter content and proteinase activity, as well as an increase in both the richness and diversity of the proteinase-producing bacterial community, compared to the adjacent non-rice soils. The Proteobacteria phylum and the Gammaproteobacteria class were dominant in sea rice soils, showing higher abundance than in the reference soils. The Planococcus genus and Bacillus-like bacterial communities were abundant in the cultivable proteinase-producing bacteria isolated from sea rice soils. Furthermore, a significant proportion of the extracellular proteinase produced by the isolated soil bacteria consisted of serine proteinases and metalloproteinases. These findings provided new insights into the degradation of soil organic nitrogen in coastal agricultural regions.

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Section: Alpha Diversity and Taxonomy Composition Analysis Of Bacteri...mentioning

confidence: 77%

Impact of Sea Rice Planting on Enzymatic Activity and Microbial Community of Coastal Soils: Focus on Proteinase

et al. 2023

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“…Blood hemoglobin is involved in oxygen and carbon dioxide transport and maintains pH balance, which is an essential indicator of a general blood test [39,40]. The data obtained indicate that with intestinal dysbiosis, anemia occurs, which is aggravated depending on the severity, and with the third degree of severity, it shows its maximum values.…”

Section: Resultsmentioning

confidence: 99%

Features of the course of compensated, subcompensated and decompensated intestinal dysbiosis in cats

Tresnitskiy,

Lutsay,

Ananev

et al. 2023

E3S Web Conf.

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The treatment of intestinal dysbiosis in animals remains one of the most complex and urgent problems in veterinary medicine to this day. Recently, a steady increase in pathologies has been recorded, accompanied by the development of disorders of the gastrointestinal tract of various etiologies in animals. Despite the constant improvement of methods for correcting the intestinal microbiota, the occurrence of dysbacteriosis in various pathological processes not only does not decrease, but, on the contrary, increases. This study revealed definite pathogenetic features of intestinal dysbiosis in cats of varying severity. It has been established that substantial changes occur in the blood of cats with dysbiosis, and with the decompensated third degree of severity, profound hematological shifts occur. The differences found may have significant clinical and laboratory importance since they complement the available data on intestinal dysbiosis in cats, which, in our opinion, will improve diagnostic and prognostic approaches, as well as therapeutic and preventive measures. Keywords: dysbiosis, cats, pathogenesis, compensated, subcompensated, decompensated.

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“…We have isolated αand β-lytic proteases, lysine-specific proteases, as well as identified new bacteriolytic proteases of MW 26 and 29 kDa, including N-acetylglucosaminidase [6]. For the β-lytic protease, the spatial structure has been established and its characterization has been expanded; an effective expression system has been developed [7,8]. The development of this system will enable us to isolate and study bacteriolytic Lysobacter enzymes with high efficiency.…”

Section: Introductionmentioning

confidence: 99%

Transcriptomic Analysis Followed by the Isolation of Extracellular Bacteriolytic Proteases from Lysobacter capsici VKM B-2533T

Afoshin,

Kudryakova,

Tarlachkov

et al. 2023

IJMS

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The aim of the study was to search for, isolate and characterize new bacteriolytic enzymes that show promising potential for their use in medicine, agriculture and veterinary. Using a transcriptomic analysis, we annotated in Lysobacter capsici VKM B-2533T the genes of known bacteriolytic and antifungal enzymes, as well as of antibiotics, whose expression levels increased when cultivated on media conducive to the production of antimicrobial agents. The genes of the secreted putative bacteriolytic proteases were also annotated. Two new bacteriolytic proteases, Serp and Serp3, were isolated and characterized. The maximum bacteriolytic activities of Serp and Serp3 were exhibited at low ionic strength of 10 mM Tris-HCl, and high temperatures of, respectively, 80 °C and 70 °C. The pH optimum for Serp was 8.0; for Serp3, it was slightly acidic, at 6.0. Both enzymes hydrolyzed autoclaved cells of Micrococcus luteus Ac-2230T, Proteus vulgaris H-19, Pseudomonas aeruginosa and Staphylococcus aureus 209P. Serp also digested cells of Bacillus cereus 217. Both enzymes hydrolyzed casein and azofibrin. The newly discovered enzymes are promising for developing proteolytic antimicrobial drugs on their basis.

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Structural and Functional Characterization of β−lytic Protease from Lysobacter capsici VKM B−2533T

Cited by 3 publications

References 39 publications

Impact of Sea Rice Planting on Enzymatic Activity and Microbial Community of Coastal Soils: Focus on Proteinase

Impact of Sea Rice Planting on Enzymatic Activity and Microbial Community of Coastal Soils: Focus on Proteinase

Features of the course of compensated, subcompensated and decompensated intestinal dysbiosis in cats

Transcriptomic Analysis Followed by the Isolation of Extracellular Bacteriolytic Proteases from Lysobacter capsici VKM B-2533T

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