Structural and functional characteristics of the pulmonary hemomicrocirculatory bed in induced systemic sclerosis: an experimental study

Doskaliuk, Bohdana; Zaiats, L. M.

doi:10.1007/s00296-023-05328-z

Cited by 4 publications

(5 citation statements)

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“…Electron microscopic studies have shown that in acute arginine-induced pancreatitis there are marked violations of the ultrastructural structure of the hemocapillaries of the alveolar wall. A number of other scientists point to changes of a similar nature under the action of exo-and endogenous factors [4,5,6].…”

Section: Resultsmentioning

confidence: 99%

Ultrastructural changes of hemocapillaries of the lungs in the late development of experimental acute pancreatitis

Zaiats,

Pasichnyk,

Zukow

2024

J Educ Health Sport

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Background. Today, acute pancreatitis is a common form of acute abdomen in the clinic, the incidence of which has been increasing in recent years. The aim of the work was to study the dynamics of ultrastructural changes of the hemocapillaries of the alveolar wall of the lungs in the late stages of experimental acute pancreatitis. Material and methods. The experiments were carried out on 54 white Wistar male rats weighing 180–220 g. The animals were divided into three groups: first — intact, second — control, third — experimental with a model of acute pancreatitis, which was reproduced by intraperitoneal administration of a 20% solution of L-arginine at a total dose of 5 g/kg at one-hour interval. The control group of animals was intraperitoneally injected with an equivalent dose of isotonic sodium chloride solution. All research were performed under sodium thiopental anesthesia at the rate of 60 mg/kg body weight. Lung tissue for electron microscopic examination was collected from the lower lobe of the left lung at 3–5 and 7 days. Pieces of lung tissue measuring 1×1×1 mm were fixed in a 2.5% glutaraldehyde solution, followed by additional fixation in a 1% osmium tetroxide solution. After dehydration, the material was poured into Epon-Araldite. Sections with a thickness of 20–50 nm obtained on “Tesla BS-490” ultramicrotome were studied in a PEM-125K electron microscope. Results. The ultrastructural analysis showed that already 3 days after the study, dystrophic-destructive changes, as well as adhesion and aggregation of leukocytes, were detected in the endothelial cells. As the study period increased (5–7 days), the intensity of changes in the hemocapillaries of the alveolar wall increased significantly. In the lumen erythrocyte sludge, thromboleukocyte adhesion and aggregation are determined in hemocapillaries. Conclusion. Acute experimental pancreatitis is accompanied by marked changes in the ultrastructural structure of hemocapillaries of the alveolar wall. The nature and severity of structural changes in the hemocapillaries of the alveolar wall depends on the duration of the course of arginine-induced acute pancreatitis.

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Section: Resultsmentioning

confidence: 99%

Ultrastructural changes of hemocapillaries of the lungs in the late development of experimental acute pancreatitis

Zaiats,

Pasichnyk,

Zukow

2024

J Educ Health Sport

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“…Finally, adult Wistar rats subcutaneously injected with NaClO were found to undergo significant histopathological changes in lung microvasculature, with capillary disruption, narrowing of the lumen, and perivascular infiltration [ 98 ]. From an ultrastructural point of view, the animals presented several changes in lung ECs, including swollen mitochondria, fragmentation of the membranes of the granular endoplasmic reticulum, uneven thickening of the basement membrane, and numerous micro pinocytotic vesicles and vacuoles [ 98 ].…”

Section: Animal Models Of Ssc Vasculopathymentioning

confidence: 99%

“…Type V Collagen Immunized Mouse EC activation and apoptosis [97] Rats Subcutaneously Injected with NaClO Capillary disruption, narrowing of vascular lumen, and perivascular infiltration in lungs [98] TNF-Transgenic Mouse Pulmonary vasculopathy; EndoMT; dysregulated angiogenic pathways [81,95] Sclerodermatous Graft-Versus-Host Disease Mouse Fibroproliferative vasculopathy [81,96] EC, endothelial cell; EndoMT, endothelial-to-mesenchymal transition; PAH, pulmonary arterial hypertension; SSc, systemic sclerosis.…”

Section: Animal Model Vascular Manifestations Referencesmentioning

confidence: 99%

Recent Insights into Cellular and Molecular Mechanisms of Defective Angiogenesis in Systemic Sclerosis

Romano,

Rosa,

Fioretto

et al. 2024

Biomedicines

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In systemic sclerosis (SSc, or scleroderma), defective angiogenesis, clinically manifesting with abnormal capillary architecture and severe capillary reduction, represents a hallmark of early-stage disease, usually preceding the onset of tissue fibrosis, and is caused by several cellular and molecular mechanisms affecting microvascular endothelial cells with different outcomes. Indeed, once damaged, endothelial cells can be dysfunctionally activated, thus becoming unable to undergo angiogenesis and promoting perivascular inflammation. They can also undergo apoptosis, transdifferentiate into profibrotic myofibroblasts, or acquire a senescence-associated secretory phenotype characterized by the release of exosomes and several profibrotic and proinflammatory mediators. In this narrative review, we aimed to give a comprehensive overview of recent studies dealing with the cellular and molecular mechanisms underlying SSc defective angiogenesis and the related endothelial cell dysfunctions, mainly the endothelial-to-mesenchymal transition process. We also discussed potential novel vascular treatment strategies able to restore the angiogenic process and reduce the endothelial-to-mesenchymal transition in this complex disease.

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“…встановили, що підвищена експресія VCAM-1 пов'язана з судинною дисфункцією та активацією ендотеліальних клітин у пацієнтів з ССД [11]. Ці дані знаходяться в повній відповідності до результатів нашого експерименту, оприлюднених раніше [10]. Нам вдалося підтвердити вищу концентрацію VCAM-1 у плазмі крові експериментальних тварин у порівнянні з контролем (91.25 [85.00-264.98] та 19.50 [13.53-22.20] відповідно, p<0.05), що є доказом активації ендотеліальних клітин на функціональному рівні в рамках цієї моделі.…”

Section: електронно-мікроскопічне дослідженняunclassified

“…Нам вдалося підтвердити вищу концентрацію VCAM-1 у плазмі крові експериментальних тварин у порівнянні з контролем (91.25 [85.00-264.98] та 19.50 [13.53-22.20] відповідно, p<0.05), що є доказом активації ендотеліальних клітин на функціональному рівні в рамках цієї моделі. Поряд із підвищенням концентрації VCAM-1 ми спостерігали наявність ремоделювання гемокапілярів легень, що супроводжувалось звуженням просвіту мікросудин унаслідок потовщення судинної стінки внаслідок набряку та периваскулярної інфільтрації поліморфноядерними клітинами [10].…”

Section: електронно-мікроскопічне дослідженняunclassified

The Complex Effect of Vitamin D and Alpha Tocopherol Acetate on the Lung Microcirculation in the Experimental Induction of Systemic Sclerosis

Doskaliuk¹,

Zaiats²

2023

Scientific and practical journal

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Abstract. The aim of the study was to investigate the effects of vitamin D3 (vitamin D) and alpha-tocopherol acetate (vitamin E) on the microcirculatory vessels of the lungs in an experimental model of systemic sclerosis (SSc). Methods. We divided the experimental animals into four separate groups: an intact group (IG) consisting of 15 animals, a control group (CG) of 20 animals, experimental group 1 (EG1) and experimental group 2 (EG2) consisting of of 25 animals each. Randomization of groups was carefully performed using the weight variance minimization method. To induce SSc in laboratory animals of EG1, subcutaneous injections of 0.5 ml of 5% sodium hypochlorite (NaClO) were administered 3 times a week for 6 weeks in a row. The CG of rats received an isotonic solution following the same scheme, while the IG was not subjected to any injections. In addition to NaClO, the EG2 received intramuscular injections of vit E (10 mg/100 g) and vit D (1000 IU/100 g) for three weeks during the second half of the experiment. After 8 weeks, the animals were euthanized, and lung tissue samples were obtained for histological and electron microscopic analysis. The histological preparations were examined using light microscopy and photographed using a Leica DME light microscope, a DCM 900 digital microscope camera, and a Nicon Coolpix P5100 camera. Meanwhile, the electron microscopy was performed to visualize the ultrastructural characteristics of the lung tissue by use of "PEM-125K" (Selmi, Ukraine) with subsequent photography at magnifications from 2000 to 20000 times. All animal procedures were carried out in strict compliance with bioethical principles. The Ethics Commission of the IFNMU granted approval for the study under document number 117/20 on November 19, 2020. Results. The histological analysis of the lung specimens of CG animals showed a normal morphological structure. In the EG1, the lungs exhibited abnormalities in the structure of the hemocapillaries, including narrowing of the vessel lumen due to thickening of the vascular wall, edema, and infiltration of polymorphonuclear cells. However, in the EG2, there was an improvement in the histological features, with a reduction in edematous processes and a decrease in the intensity of polymorphonuclear cell infiltration. The electron microscopic examination of the lung samples from EG1 animals revealed the presence of platelet adhesion and aggregation, as well as aggregates of erythrocytes within the hemocapillary lumen. Swelling of endothelial cells and narrowing of the hemocapillary lumen were also observed, along with uneven thickening of the basement membrane. In contrast, the combination of vitamins D and E in EG2 animals resulted in improved rheological properties of blood and ultrastructural characteristics of the hemomicrocirculatory elements. The lumen of the hemocapillaries contained single erythrocytes and inactive platelets. The endothelial cells’ membraines showed minimal alterations, with clear contours and no micropinocytotic vesicles or cytoplasmic growths. The nuclei of the endothelial cells had a fine-grained matrix with evenly distributed chromatin granules. Conclusions. This study proves that the combined use of vitamins D and E has a positive effect on the state of the microcirculatory vessels in the lungs of experimentally induced systemic sclerosis.

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Structural and functional characteristics of the pulmonary hemomicrocirculatory bed in induced systemic sclerosis: an experimental study

Cited by 4 publications

References 28 publications

Ultrastructural changes of hemocapillaries of the lungs in the late development of experimental acute pancreatitis

Ultrastructural changes of hemocapillaries of the lungs in the late development of experimental acute pancreatitis

Recent Insights into Cellular and Molecular Mechanisms of Defective Angiogenesis in Systemic Sclerosis

The Complex Effect of Vitamin D and Alpha Tocopherol Acetate on the Lung Microcirculation in the Experimental Induction of Systemic Sclerosis

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