Structural and functional characteristics of irradiation damage to parotid glands in the miniature pig

Li, Jun; Zhang, Shan; Ou, Guangfei; Liu, Xiaoyong; Zhang, Chunmei; Baum, Bruce J.; Wang, Songlin

doi:10.1016/j.ijrobp.2005.04.029

Cited by 62 publications

(84 citation statements)

References 37 publications

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“…Additional improvements should be made in future studies, including the determination of proper cell numbers for each scaffold, the optimization of the recellularization process and the orthotopic transplantation of bioengineered salivary glands in vivo. A large animal model, such as the miniature pig, which has a similar salivary gland anatomy to a human, could be used in transplantation and saliva secretion experiments in the future [Li et al, 2004[Li et al, , 2005.…”

Section: Discussionmentioning

confidence: 99%

Generation of Bioartificial Salivary Gland Using Whole-Organ Decellularized Bioscaffold

Gao

et al. 2014

Cells Tissues Organs

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Salivary gland hypofunction resulting in xerostomia occurs as a result of various pathological conditions such as radiotherapy for head and neck cancers, Sjögren's syndrome or salivary gland tumor resection. It can induce a large number of problems, including dental decay, periodontitis, dysgeusia, difficulty with mastication and swallowing and a reduced quality of life. Current therapies for xerostomia mostly focus on saliva substitutes, oral lubricants and medications which stimulate salivation from residual glands. However, these treatments are not sufficient to restore gland secretory function. Tissue engineering-based organ regeneration has emerged as a potential therapeutic alternative for end- organ failure. Here, we decellularized rat submandibular glands (SMG) by detergent immersion. Histological, immunofluorescent, Western blot, DNA and collagen quantitative analyses demonstrated that our protocol effectively removed cellular components and that extracellular matrix proteins and native structures were well preserved. We then reseeded the decellularized SMG as scaffolds with rat primary SMG cells in a rotary cell culture system. Histological staining and electron microscopy analyses illustrated that the decellularized SMG could support cellular adhesion. Furthermore, with immunofluorescent staining, we proved that bioartificially generated SMG showed some differentiation markers in vitro. Taken together, our findings might provide a potential scaffold for tissue-engineered regeneration of the salivary glands.

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Section: Discussionmentioning

confidence: 99%

Generation of Bioartificial Salivary Gland Using Whole-Organ Decellularized Bioscaffold

Gao

et al. 2014

Cells Tissues Organs

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“…Sialometric studies disclose reduction of salivary flow in the acute setting [3] as well as in the late stage [3,4] after head and neck radiation. On histopathological studies, acute radiation injuries present as swelling, degeneration and necrosis of acinar cells [5], while late radiation injuries further demonstrate loss of acinar cells, dilatation of intercalated ducts, fibrosis and atrophy of parotid lobules [4,5]. The functional impairment and morphological damage of the parotid glands can persist as long as 24 months after the irradiation [3].…”

Section: Introductionmentioning

confidence: 99%

“…Late radiation injuries of the parotid glands usually present as transient or permanent xerostomia, which further increases the risk for developing dental caries, compromises the oral mucosal integrity, and results in oral pain, loss of taste, difficulty with swallowing and chewing, sleep disorders and worse quality of life [2]. Sialometric studies disclose reduction of salivary flow in the acute setting [3] as well as in the late stage [3,4] after head and neck radiation. On histopathological studies, acute radiation injuries present as swelling, degeneration and necrosis of acinar cells [5], while late radiation injuries further demonstrate loss of acinar cells, dilatation of intercalated ducts, fibrosis and atrophy of parotid lobules [4,5].…”

Section: Introductionmentioning

confidence: 99%

Perfusion characteristics of late radiation injury of parotid glands: quantitative evaluation with dynamic contrast-enhanced MRI

Juan¹,

Chen²,

Jen³

et al. 2008

Eur Radiol

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We aimed to quantitatively investigate the alteration of parotid perfusion after irradiation using dynamic contrast-enhanced magnetic resonance imaging (DCE-MRI) based on a two-compartment tracer kinetic model. This study enrolled 19 patients (53.2 +/- 14.9 years) treated by head and neck radiotherapy and 19 age-relevant and sex-matched subjects as a control group. Perfusion parameters (K ( el ), k (21) and A) of parotid glands were analyzed based on the Brix model from T1-weighted DCE-MRI. Suitability of the Brix model was evaluated via Monte Carlo simulation for the goodness-of-fit. Analysis of nonlinear goodness-of-fit showed that the Brix model is appropriate in evaluating the parotid perfusion (R(2) = 0.938 +/- 0.050). The irradiated parotid glands showed significantly lower K ( el ) (P < 0.0005) and k (21) (P < 0.05) and consequently significantly higher value of peak enhancement (P < 0.0005) and time-to-peak (P < 0.0005) compared with non-irradiated ones, suggestive of gradual and prolonged accumulation and delayed wash-out of contrast agent due to increased extracellular extravascular space and decreased vascular permeability in the irradiated glands. Linear regression analysis showed dose-dependent perfusion changes of the irradiated parotid glands. We conclude that quantitative DCE-MRI is a potential tool in investigating parotid gland perfusion changes after radiotherapy.

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“…Un mois après la fin de la radiothérapie, ils se plaignaient toujours de sécheresse buccale. L'irradiation des glandes salivaires, et notamment l'irradiation des glandes parotides, avec des doses supérieures à 60 Gy, se traduit par une atrophie des acinus, une nécrose intra-glandulaire, des altérations vasculaires, une fibrose et une dégénérescence graisseuse [8].…”

Section: Sécrétion Salivaire Et Pouvoir Tamponunclassified

Évaluation quantitative de la secrétion et du pouvoir tampon de la salive au cours de la radiothérapie des cancers des voies aéro-digestives supérieures au Sénégal

Lecor

Dia-Tine

Gaye

et al. 2013

Med Buccale Chir Buccale

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Résumé -Introduction. La radiothérapie, ou utilisation des rayonnements ionisants pour détruire le tissu cancé-reux, représente un des traitements de base pour les cancers des voies aéro-digestives (VADS). Elle doit détruire les cellules cancéreuses, tout en épargnant au mieux les organes ou tissus sains avoisinants. L'objectif de cette étude était d'évaluer les effets de la radiothérapie sur la fonction des glandes salivaires chez des patients traités pour un cancer des VADS. Matériel et méthodes. Une étude transversale descriptive a été réalisée sur 33 patients atteints d'un cancer des VADS et traités dans le service de radiothérapie de l'Hôpital Aristide Le Dantec de Dakar (Sénégal). La xérostomie, les débits salivaires non stimulé et stimulé ainsi que le pH salivaire ont été évalués, avant (T0), pendant (T), et un mois après la fin de la radiothérapie (T1). Les données ont été statistiquement analysées et exploitées en utilisant le logiciel SPSS. Résultats. L'âge des patients variait de 18 à 69 ans. Les patients ayant plus de 60 ans représentaient 45,5 % de la population étudiée. Les hommes constituaient la majorité de l'échantillon (54,5 %). Avant la radiothérapie, aucun signe de xérostomie n'a été observé chez les patients : tous avaient un débit salivaire normal. Pendant les séances de radiothérapie, tous les patients se sont plaints de sécheresse buccale : 19 avaient une hyposialie modérée et 13 avaient une hyposialie sévère. La xérostomie et l'hyposialie sévère ou modérée étaient statistiquement associées à la dose de radiothérapie (p < 0,005). À T0, tous les patients avaient un pH neutre. À T1, 28/33 patients avaient un pH salivaire inférieur à 5,5, évoquant une hyposialie. Conclusion. L'orientation vers de nouvelles techniques d'irradiation de la tête et du cou pour épargner partiellement les glandes salivaires ouvre des voies potentielles de recherche et d'innovation pour les années à venir.Abstract -Quantitative evaluation of the secretion and buffer capacity of saliva during radiotherapy for cancers of the upper aerodigestive tract in Senegal. Introduction. Radiation therapy is a common treatment for head and neck cancers, which uses ionizing radiation, to destroy cancerous tissue. Its purpose is the sterilization of cancer cells while sparing the best surrounding healthy tissue or organs. The objective of this study was to evaluate the effects of radiotherapy on salivary gland function in patients treated for head and neck cancer. Material and methods. A descriptive cross-sectional study was performed repeatedly in 33 patients with head and neck cancer and treated with radiotherapy department of the Aristide Le Dantec Hospital in Dakar (Senegal). Xerostomia, salivary flow unstimulated and stimulated saliva and pH were measured before (T0), during (T), and one month after radiotherapy (T1). The data were statistically analyzed and evaluated using the SPSS software. Results. The extreme age of patients was 18 and 69 years. People over 60 years old accounted for 45.5% of the population. Men constitut...

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Structural and functional characteristics of irradiation damage to parotid glands in the miniature pig

Cited by 62 publications

References 37 publications

Generation of Bioartificial Salivary Gland Using Whole-Organ Decellularized Bioscaffold

Generation of Bioartificial Salivary Gland Using Whole-Organ Decellularized Bioscaffold

Perfusion characteristics of late radiation injury of parotid glands: quantitative evaluation with dynamic contrast-enhanced MRI

Évaluation quantitative de la secrétion et du pouvoir tampon de la salive au cours de la radiothérapie des cancers des voies aéro-digestives supérieures au Sénégal

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