Structural and functional change of blood vessel labyrinth in maturing placenta of mice

Suzuki, K.; Kobayashi, Miya; Kobayashi, Kunihiko; Shiraishi, Yuma; Goto, Setsuko; Hoshino, Takao

doi:10.1016/s0143-4004(05)80169-9

Cited by 12 publications

(14 citation statements)

References 7 publications

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“…Interestingly, the levels of caspase-3 activity on day 8 were ϳ10 times higher than on day 14, which might mirror the remodeling processes taking place after implantation and during placental growth. On day 14, on the contrary, the placenta is already formed and does not further grow, whereas the fetus grows very fast (22). The analysis of apoptotic cells as measured by TUNEL assay on day 14 of pregnancy reveals that the apoptosis rate is tendencially down-regulated in the Co-PP-treated group compared with the PBS-treated abortion group (Fig.…”

Section: Ho-1 Up-regulation Led To Slightly Diminished Apoptosis Whimentioning

confidence: 89%

“…Accordingly, the Zn-PP treatment significantly boosted the abortion rate of abortion-prone mice from 20 to 30%, further confirming a negative effect of HO-1 down-regulation or absence during implantation window on pregnancy outcome. We performed a second set of experiments, sacrificing the animals 4 days after treatment, i.e., on day 8 of pregnancy; however, the abortion rate of these animals could not be registered, because it has been not possible to distinguish between resorption and healthy implantation sites, as already described in literature (20,22). The implantation rates were comparable among all groups (Fig.…”

Section: The Up-regulation Of Ho-1 By Co-pp Application During Implanmentioning

confidence: 97%

“…A second set of experiments was conducted, and the animals were sacrificed on day 8: PBS-treated DBA/2J-mated CBA/J females (n ϭ 4); Co-PP-treated DBA/2J-mated CBA/J females (n ϭ 5); and Zn-PP-treated DBA/2J-mated CBA/J females (n ϭ 5). It is important to remark that on day 8 of pregnancy it was impossible to distinguish between rejection and normal implantation sites, because abortion events take place on day 8 -9 of pregnancy (22). For flow cytometry, decidual and spleen tissue were collected in HBSS.…”

Section: Animals and Samples Collectionmentioning

confidence: 99%

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Protection from Abortion by Heme Oxygenase-1 Up-Regulation Is Associated with Increased Levels of Bag-1 and Neuropilin-1 at the Fetal-Maternal Interface

Sollwedel

Bertoja

Zenclussen

et al. 2005

The Journal of Immunology

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Tolerance mechanisms allowing pregnancy success resemble those involved in allograft acceptance. Heme oxygenase (HO) is a tissue-protective molecule, which allows graft acceptance and is known to have antiapoptotic effects on several cell types. We previously reported down-regulated levels of HO-1 and HO-2 in placenta from allopregnant mice undergoing abortion. In this study, we analyzed whether the up-regulation of HO-1 by cobalt-protoporphyrin (Co-PP) during implantation window can rescue mice from abortion. Induction of HO-1 by Co-PP treatment prevented fetal rejection, whereas the down-regulation of HOs by zinc-protoporphyrin application boosted abortion. The beneficial effect of HO-1 induction was not related to a local shift to Th2-profile or to a change in the NO system. Interestingly, the expression of the antiapoptotic/cytoprotective molecule Bag-1 as well as the levels of neuropilin-1, a novel marker for T regulatory cells, were up-regulated after Co-PP treatment. Our data strongly support a very important role for HO-1 in fetal allotolerance and suggest that HO-1 might be protective by up-regulating tissue protective molecules, i.e., Bag-1, and by activating T regulatory cells rather than by changing the local cytokine profile.

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Section: Ho-1 Up-regulation Led To Slightly Diminished Apoptosis Whimentioning

confidence: 89%

Section: The Up-regulation Of Ho-1 By Co-pp Application During Implanmentioning

confidence: 97%

Section: Animals and Samples Collectionmentioning

confidence: 99%

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Protection from Abortion by Heme Oxygenase-1 Up-Regulation Is Associated with Increased Levels of Bag-1 and Neuropilin-1 at the Fetal-Maternal Interface

Sollwedel

Bertoja

Zenclussen

et al. 2005

The Journal of Immunology

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“…Defective placental development as well as spiral artery formation cause fetal growth retardation (Ashkar et al 2000, Song et al 2002, Watson & Cross 2005 as both structures are crucial for maternal and fetal blood exchange, and therefore, for supplying the fetus with oxygen and nutrients. Even at day 14 of pregnancy when the fetus but not the placenta needs to grow (Suzuki et al 1997), the amount of uMCs is still considerable although significantly lower when compared with that at estrus. These observations are indicative of the involvement of uMCs in pregnancy establishment rather than its maintenance.…”

Section: In Vivo Visualization Of Uterine Mast Cellsmentioning

confidence: 96%

“…At gestational day 10 when the placenta and the fetus are simultaneously growing, uMCs could be still detected mesometrially but their number was significantly lower than at estrus ( Figs 3E and 4). At mid-gestation (day 14 of pregnancy) when the main focus is on fetal rather than on placental development (Suzuki et al 1997), uMCs are present but in significantly lower numbers when compared with estrus ( Figs 3F and 4). …”

Section: A B Estrus Depth 105 µMmentioning

confidence: 99%

In vivo visualization of uterine mast cells by two-photon microscopy

Schmerse¹,

Woidacki²,

Riek‐Burchardt³

et al. 2014

Reproduction

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Transgenic mice expressing fluorescent proteins in specific cell populations are widely used for the study of in vivo behavior of these cells. We have recently reported that uterine mast cells (uMCs) are important for implantation and placentation. However, their in vivo localization in uterus before and during pregnancy is unknown. Herein, we report the direct observation of uMCs in vivo using doubletransgenic C57BL/6J Mcpt5-Cre ROSA26-EYFP mice with high expression of enhanced yellow fluorescent protein in MC protease 5 (Cma1 (Mcpt5))-expressing cells by intravital two-photon microscopy. We were able to monitor MCs live in utero during the murine estrous cycle and at different days of pregnancy. We demonstrated that uMCs accumulated during the receptive phase of the female (estrus) and persisted in large numbers at early pregnancy stages and around mid-gestation and declined in number in non-pregnant animals at diestrus. This intravital microscopy technique, including a custom-made microscope stage and the adaption of the surgical procedure, allowed the access of the uterus and implantations for imaging. The introduced application of intravital microscopy to C57BL/6J-Mcpt5-Cre ROSA26-EYFP mice offers a novel and powerful in vivo approach to further address the evident relevance of uMCs to reproductive processes with obvious clinical implications.

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Developmental effects of oral exposure to diethylstilbestrol on mouse placenta

Nagao

Kagawa

Saito

et al. 2012

J of Applied Toxicology

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Placental growth and function are of biological significance in that placental tissue promotes prenatal life and the maintenance of pregnancy. Exposure to synthetic estrogens causes embryonic mortality and placental growth restriction in mice. The aim of the present study was to examine the effects of diethylstilbestrol (DES) on placenta in mice. DES at 1, 5, 10 or 15 µg kg(-1) day(-1) , or 17β-estradiol (E2 ) at 50 µg kg(-1) day(-1) , was administered orally to ICR mice on days 4 through to 8 of gestation. Expression of ERα, ERβ, ERRβ or ERRγ mRNA in the junctional or labyrinth zone of the placentas on day 13 was assessed using RT-PCR, as well as the embrynic mortality, embryonic and placental weight, histological changes of labyrinth and ultrastructural changes of the trophoblast giant cells (TGCs). Embryo mortalities in the DES 10 and 15 µg kg(-1) day(-1) groups were markedly increased. No significant changes in embryonic and placental weight were observed in any DES- or E2 -exposed groups. Expression of ERα mRNA in the junctional zone with male embryos in the 5 µg kg(-1) day(-1) group was significantly higher than that in the control, whereas expression was not determined in the 15 µg kg(-1) day(-1) group. Histological observation revealed that the placentas exposed to DES at 10 µg kg(-1) day(-1) lacked the developing labyrinth. Ultrastructural observation of the TGCs showed poor rough-surfaced endoplasmic reticulum in the DES 10 µg kg(-1) day(-1) group. The present data suggest that developmental changes induced by DES may be related to interference with the nutrition and oxygen exchange between mother and embryo or decreased protein synthesis, resulting in a high frequency of embryo mortality.

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Structural and functional change of blood vessel labyrinth in maturing placenta of mice

Cited by 12 publications

References 7 publications

Protection from Abortion by Heme Oxygenase-1 Up-Regulation Is Associated with Increased Levels of Bag-1 and Neuropilin-1 at the Fetal-Maternal Interface

Protection from Abortion by Heme Oxygenase-1 Up-Regulation Is Associated with Increased Levels of Bag-1 and Neuropilin-1 at the Fetal-Maternal Interface

In vivo visualization of uterine mast cells by two-photon microscopy

Developmental effects of oral exposure to diethylstilbestrol on mouse placenta

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