Structural and functional analysis of three D/L-like class I molecules from H-2v: indications of an ancestral family of D/L genes.

Cai, Zeling; Pease, Larry R.

doi:10.1084/jem.175.2.583

Cited by 7 publications

(3 citation statements)

References 42 publications

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“…An intermediate level of B27 expression was observed in the H-2v haplotype (Nickerson et al 1990) which has a D region organization distinct from the five-gene organization observed in the D d region, and from the H-2 b prototype with only one H-2D gene (Cai and Pease 1992;Stephen et al 1986;Weiss et al 1984;Duran et al 1989). The pattern of expression of the three nonclassical class I genes (D2-D3-D4) mapping between H-2D and H-2L is not known, but it has been reported that some of them can be transcribed and expressed, although with low stability (Cai and Pease 1992;Hedley et al 1990). Thus, transcribed pseudogenes could be using [32m, further limiting the amount of ~32m available.…”

Section: Discussionmentioning

confidence: 99%

Hierarchy in the assembly of HLA-B27 and HLA-Cw3 molecules in transgenic mice

Pedrinaci¹,

Hanson²,

David³

1994

Immunogenetics

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Cell surface expression of human class I molecules in transgenic mice is dependent upon the available pool of beta 2-microglobulin (beta 2m) and the affinity between mouse beta 2m and human class I molecules. HLA-B27 and HLA-Cw3 transgenes can be expressed in mouse strains of the H-2 haplotypes b, f, k, and s which encode two endogenous class I genes mapping to H-2K and H-2D. The human class I genes cannot be expressed on H-2d and H-2q haplotypes which encode three endogenous class I molecules (K,D,L). This suggests that there may be only enough mouse beta 2m molecules to support three class I molecules. When both the HLA-B27 and HLA-Cw3 genes are introduced into H-2b mice, only HLA-Cw3 reaches the cell surface. This suggests that HLA-Cw3 has a higher affinity than HLA-B27 for mouse beta 2m. The possible implications of our findings regarding the assembly, transport, and expression of class I MHC molecules in vivo are discussed.

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Section: Discussionmentioning

confidence: 99%

Hierarchy in the assembly of HLA-B27 and HLA-Cw3 molecules in transgenic mice

Pedrinaci¹,

Hanson²,

David³

1994

Immunogenetics

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“…The latter is specifically designed for the purification of products ~200 bp. Alternatively, purification may also be achieved by electroelution (10) or by a modification of the freeze-squeeze technique. (2~ To purify DNA by the freeze-squeeze method, the band of DNA fragment of interest is excised from an agarose gel, macerated, transferred to a microcentrifuge tube, and frozen at -70~ for 1 hr (or overnight at -20~ The frozen gel is thawed at 37~ for I hr, transferred to an Ultrafree-MC filter unit (Millipore, Bedford, MA) or a Spin-X filter unit (Costar, Cambridge, MA), and centrifuged at 14,000 rpm for 20 min at room temperature.…”

Section: S Purification Of Pcr and $Oeing Productsmentioning

confidence: 99%

In vitro synthesis of novel genes: mutagenesis and recombination by PCR.

Vallejo

Pogulis

Pease³

1994

Genome Research

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“…The sequence data support such a relationship among these class I genes (Rubocki et al 1990;Lee et al 1988). Recently, Cai and Pease (1992) isolated three distinct H-2 v cDNA clones, all of which share the characteristics of D/L locus genes, and suggested that the D and L loci may have their origin in an ancestral family of D/L loci, rather than in a single ancestral locus such as the D. The presence of a Bat-lb gene in this haplotype suggests that the same unequal recombination was involved at some point in the evolution of all three haplotypes, d, q, and v.…”

mentioning

confidence: 99%