Structural and functional analysis of Aspergillus niger xylanase to be employed in polyethylenglycol/salt aqueous two-phase extraction

Loureiro, Dana Belén; Romanini, Diana; Tubío, Gisela

doi:10.1016/j.bcab.2015.12.008

Cited by 11 publications

(3 citation statements)

References 24 publications

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“…The systems were carried out in Eppendorf tubes with the following characteristics: volume ratio (VR) equal to 1, the weight of 1.8 g, constant pH 7, and four tie-line length (TLL)-15, 25, 35, and 45 %w/w. TLL is defined as the mass concentration of the chemicals forming PEG-phosphate in the top and bottom phase [20]. Three different PEG molecular masses were employed (1000, 3350, and 8000 KDa).…”

Section: Atps Preparation and Xylanase Partitioningmentioning

confidence: 99%

“…Bioseparation techniques such as aqueous two-phase biosystems (ATPS) are a potential option for the initial protein purification step [18]. ATPS are well-studied methodologies and ideal for the recovery of enzymes due to high water content requirements and low interphase tension, which provides favourable conditions for the preservation of biomolecules [19,20]. Several enzymes such as lipases, proteases, alphaamylase, laccase, and xylanase [15,21,22], among others, have been partially purified through these systems obtaining high recovery yields and considerable levels of purification.…”

Section: Introductionmentioning

confidence: 99%

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Production of a xylanase by Trichoderma harzianum (Hypocrea lixii) in solid-state fermentation and its recovery by an aqueous two-phase system

Gómez-García¹,

Medina-Morales²,

Rodríguez³

et al. 2018

Can J Biotech

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Production of xylanase enzyme by fungi strains has gained interest in the recent years due to its high productivity, high catalytic power, as well as its potential applications in different areas such as feed, food, textile, and biofuel industries. The conventional methodologies, to produce enzymes, involve complex apparatus and chemical solvents and are associated with high costs and lowyield recovery. To obtain the high-yield recovery of the enzymes, modern enzyme extraction methods are taken into account. Aqueous two-phase systems (ATPS) are an alternative separative methodology for the purification and recovery of the enzymes and other biomolecules. The advantages of ATPS are easy scale-up and extraction, volume reduction, and rapid separation. The objective of this study was to produce Trichoderma harzianum xylanase by solid-state fermentation (SSF) using corn cobs as a support/substrate and employing ATPS for its partial recovery. In this study, the results showed the ability of a microorganism to grow on the corn cobs and to produce the xylanase enzyme. Xylanolytic activity reached 7.85 U/g of corn cobs. The enzyme was efficiently concentrated by ATPS. In addition, a high purification factor (10-fold) and considerable enzyme recovery (%ER) (84%) percentage were obtained.

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Section: Atps Preparation and Xylanase Partitioningmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Production of a xylanase by Trichoderma harzianum (Hypocrea lixii) in solid-state fermentation and its recovery by an aqueous two-phase system

Gómez-García¹,

Medina-Morales²,

Rodríguez³

et al. 2018

Can J Biotech

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show abstract

“…Specially, Aspergillus and Trichoderma species have been studied extensively for their ability to secrete high-levels of enzymes in solid-state fermentation (SSF) [ 12 , 13 , 14 , 15 , 16 ]. Aspergillus is a genus usually employed to produce xylanolytic enzyme cocktails in industrial plants for applications like paper manufacturing, bread-making, animal feed, juice and wine industries [ 17 , 18 ] and, thus, several studies reporting enzymatic production optimization have been released, either by traditional One-Factor-at-a-Time (OFAT) approach [ 19 ] or by Response Surface Methodology (RSM) [ 20 , 21 ]. However, since the enzymatic system secreted by fungi depends on the strain and the culture conditions, it is interesting to study new isolates to produce efficient and robust enzymes.…”

Section: Introductionmentioning

confidence: 99%

Optimization of β-1,4-Endoxylanase Production by an Aspergillus niger Strain Growing on Wheat Straw and Application in Xylooligosaccharides Production

et al. 2021

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Plant biomass constitutes the main source of renewable carbon on the planet. Its valorization has traditionally been focused on the use of cellulose, although hemicellulose is the second most abundant group of polysaccharides on Earth. The main enzymes involved in plant biomass degradation are glycosyl hydrolases, and filamentous fungi are good producers of these enzymes. In this study, a new strain of Aspergillus niger was used for hemicellulase production under solid-state fermentation using wheat straw as single-carbon source. Physicochemical parameters for the production of an endoxylanase were optimized by using a One-Factor-at-a-Time (OFAT) approach and response surface methodology (RSM). Maximum xylanase yield after RSM optimization was increased 3-fold, and 1.41- fold purification was achieved after ultrafiltration and ion-exchange chromatography, with about 6.2% yield. The highest activity of the purified xylanase was observed at 50 °C and pH 6. The enzyme displayed high thermal and pH stability, with more than 90% residual activity between pH 3.0–9.0 and between 30–40 °C, after 24 h of incubation, with half-lives of 30 min at 50 and 60 °C. The enzyme was mostly active against wheat arabinoxylan, and its kinetic parameters were analyzed (Km = 26.06 mg·mL−1 and Vmax = 5.647 U·mg−1). Wheat straw xylan hydrolysis with the purified β-1,4 endoxylanase showed that it was able to release xylooligosaccharides, making it suitable for different applications in food technology.

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Advantages and Progress Innovations of Solid-State Fermentation to Produce Industrial Enzymes

Londoño-Hernández

Ruíz

Toro

et al. 2020

Microorganisms for Sustainability

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Structural and functional analysis of Aspergillus niger xylanase to be employed in polyethylenglycol/salt aqueous two-phase extraction

Cited by 11 publications

References 24 publications

Production of a xylanase by Trichoderma harzianum (Hypocrea lixii) in solid-state fermentation and its recovery by an aqueous two-phase system

Production of a xylanase by Trichoderma harzianum (Hypocrea lixii) in solid-state fermentation and its recovery by an aqueous two-phase system

Optimization of β-1,4-Endoxylanase Production by an Aspergillus niger Strain Growing on Wheat Straw and Application in Xylooligosaccharides Production

Advantages and Progress Innovations of Solid-State Fermentation to Produce Industrial Enzymes

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